DOI: 10.1111/j.1432-1033.1992.tb16587.x. Primary structure and import pathway of the rotenone‐insensitive NADH‐ubiquinone oxidoreductase of mitochondria from <i>Saccharomyces cerevisiae</i>

Primary structure and import pathway of the rotenone‐insensitive NADH‐ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae

10.1111/j.1432-1033.1992.tb16587.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The gene encoding the yeast mitochondrial rotenone‐insensitive internal NADH: ubiquinone‐6 oxidoreductase has been sequenced. The DNA sequence indicates the presence of an open reading frame of 1539 bp predicted to encode a protein of 513 amino acid residues (57.2 kDa). The NADH dehydrogenase is synthesized as a precursor protein containing a signal sequence of 26 residues. In vitro import experiments show that the precursor NADH dehydrogenase is cleaved to the mature size by the matrix processing peptidase. Both cleavage and translocation across the mitochondrial membrane(s) are dependent on the membrane potential component of the proton‐motive force. Comparison of the protein sequence of the yeast NADH dehydrogenase with the data bank indicates that the enzyme from yeast is homologous to the NADH dehydrogenase of Escherichia coli (22.2% identical residues). Both NADH dehydrogenases contain in the central part of the protein a sequence predicted to fold into a βαβ structure involved in the binding of NADH or FAD(H2). Various aspects of the protein structure are discussed.

Bibliography

DE VRIES, S., VAN WITZENBURG, R., GRIVELL, L. A., & MARRES, C. A. M. (1992). Primary structure and import pathway of the rotenoneâinsensitive NADHâubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae. European Journal of Biochemistry, 203(3), 587â592. Portico.

Authors 4

Simon DE VRIES (first)
Richard VAN WITZENBURG (additional)
Leslie A. GRIVELL (additional)
Carla A. M. MARRES (additional)

References 46 Referenced 89

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Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 1:19 a.m.)
Deposited	1 year, 10 months ago (Oct. 31, 2023, 1:14 a.m.)
Indexed	2 months, 1 week ago (June 27, 2025, 6:35 a.m.)
Issued	33 years, 7 months ago (Feb. 1, 1992)
Published	33 years, 7 months ago (Feb. 1, 1992)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	33 years, 7 months ago (Feb. 1, 1992)

Funders 0

None

BibTeX

@article{DE_VRIES_1992, title={Primary structure and import pathway of the rotenone‐insensitive NADH‐ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae}, volume={203}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1992.tb16587.x}, DOI={10.1111/j.1432-1033.1992.tb16587.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={DE VRIES, Simon and VAN WITZENBURG, Richard and GRIVELL, Leslie A. and MARRES, Carla A. M.}, year={1992}, month=feb, pages={587–592} }

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