DOI: 10.1111/j.1432-1033.1991.tb16352.x. Tracking of proton flow during transition from anaerobiosis to steady state

Tracking of proton flow during transition from anaerobiosis to steady state

10.1111/j.1432-1033.1991.tb16352.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

1. During aerobic cation uptake in liver mitochondria, the hydrophobic pH indicator bromothymol blue undergoes a multiphase response: phase 1 (rapid acidification), phase 2 (slow alkalinization), phase 3 (rapid alkalinization) and phase 4 (reacidification).2. Titrations with ruthenium red and malonate indicate that the various phases depend on the relative rates of cation uptake and proton translocation: at high rates of cation uptake, phase 1 disappears and phases 2 and 3 are transformed in a monotonic process of alkalinization.3. The comparison of the bromothymol blue response with the arsenazo III, 2′,7′‐bis(carboxyethyl)‐5(6)carboxyfluorescein (BCECF) and safranine responses indicates that: (a) phase 2 (slow alkalinization) corresponds to a slow rise of matrix pH and a parallel decline of membrane potential; (b)phase 3 (rapid alkalinization) corresponds to termination of proton translocation and initiation of the processes of cation efflux and proton reuptake. All the above processes reach completion during phase 4.4. Although bromothymol blue always behaves as a membrane‐bound indicator, the extent to which it reflects the matrix or the cytosolic pH is a function of the membrane‐potential‐determined asymmetric distribution: in parallel with the lowering of the membrane potential, the dye chromophore is shifted from the cytosolic to the matrix side membrane layer.5. A model is discussed which describes the behaviour of bromothymol blue as pH indicator recording the changes in membrane layers facing either the matrix or the cytosolic side. The complex response of the dye during cation uptake is due to two independent processes, one of pH change and another of dye intramembrane shift. Computer simulations of the dye response, based on the conversion of a kinetic model into an electrical network and closely reproducing the experimental observations, are reported.

Bibliography

LUVISETTO, S., COLA, C., SCHMEHL, I., & AZZONE, G. F. (1991). Tracking of proton flow during transition from anaerobiosis to steady state. European Journal of Biochemistry, 202(1), 121â130. Portico.

Authors 4

Siro LUVISETTO (first)
Carmen COLA (additional)
Ibolya SCHMEHL (additional)
Giovanni Felice AZZONE (additional)

References 20 Referenced 3

{'key': 'e_1_2_2_2_2', 'first-page': '212', 'author': 'Chance B.', 'year': '1966', 'journal-title': 'Nature'} / Nature by Chance B. (1966)
10.1016/S0021-9258(18)99690-0
10.1016/S0021-9258(18)96200-9
10.1111/j.1469-185X.1966.tb01501.x
10.1016/0005-2728(90)90113-I
10.1007/BF01868043
10.1111/j.1432-1033.1991.tb16351.x
10.1021/ja00882a004
10.1002/ar.1091710302
10.1042/bj2460271
10.1111/j.1432-1033.1968.tb00166.x
10.1021/bi00342a012
10.1016/0005-2736(86)90210-5
10.1016/0005-2728(90)90196-B
{'key': 'e_1_2_2_16_2', 'first-page': '257', 'volume-title': 'Membrane Biophysics II: physical methods in the study of epithelia', 'author': 'Mikulecky D. C.', 'year': '1983'} / Membrane Biophysics II: physical methods in the study of epithelia by Mikulecky D. C. (1983)
10.1016/0304-4157(90)90005-W
10.1016/0301-4622(88)85009-9
10.1016/S0006-3495(79)85295-9
10.1016/0010-468X(82)90056-3
10.1016/0163-7258(81)90045-0

Dates

Type	When
Created	20 years, 6 months ago (March 4, 2005, 12:34 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:49 a.m.)
Indexed	1 month, 1 week ago (July 25, 2025, 6:15 a.m.)
Issued	33 years, 10 months ago (Nov. 1, 1991)
Published	33 years, 10 months ago (Nov. 1, 1991)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	33 years, 10 months ago (Nov. 1, 1991)

Funders 0

None

BibTeX

@article{LUVISETTO_1991, title={Tracking of proton flow during transition from anaerobiosis to steady state: 2. Effect of cation uptake on the response of a hydrophobic membrane bound pH indicator}, volume={202}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1991.tb16352.x}, DOI={10.1111/j.1432-1033.1991.tb16352.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={LUVISETTO, Siro and COLA, Carmen and SCHMEHL, Ibolya and AZZONE, Giovanni Felice}, year={1991}, month=nov, pages={121–130} }

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