DOI: 10.1111/j.1432-1033.1991.tb16305.x. Molecular cloning and characterisation of a novel putative protein‐serine kinase related to the cAMP‐dependent and protein kinase C families

Molecular cloning and characterisation of a novel putative protein‐serine kinase related to the cAMP‐dependent and protein kinase C families

10.1111/j.1432-1033.1991.tb16305.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Highly degenerate oligonucleotide primers designed from regions conserved between protein‐serine kinases have been used specifically to amplify human epithelial (HeLa) cDNA by the polymerase chain reaction (PCR). Of several novel cDNA fragments encodingputative kinases thus isolated, one was further characterised. Screening of human fibroblast and bovine brain cDNA libraries with the PCR fragment yielded several clones with an open reading frame of 479 amino acids containing all of the conserved sequence motifs of protein‐serine kinases. The predicted protein was most similar to the protein kinase C (PKC)/cAMP‐dependent protein kinase (PKA) families and its gene has thus been termedPkb. Expression of thePkb gene is general but highest in brain, heart and lung. Translation ofPkb RNAin vitro generated a 57‐kDa protein (PKB) recognised by antisera raised to a bacterially expressed PKB/TrpE fusion protein. Transfection of COS cells with the kinase cDNA resulted in the synthesis of a 60‐kDa protein which was partially purified purified by Mono Q anion‐exchange chromatography. Column fractions containing PKB‐immunoreactive protein exhibited elevated histone H1 kinase activity compared with similar fractions from control cells, demonstrating the enzymatic activity of this protein kinase.

Bibliography

COFFER, P. J., & WOODGETT, J. R. (1991). Molecular cloning and characterisation of a novel putative proteinâserine kinase related to the cAMPâdependent and protein kinase C families. European Journal of Biochemistry, 201(2), 475â481. Portico.

Authors 2

Paul J. COFFER (first)
James R. WOODGETT (additional)

References 32 Referenced 330

10.1038/332166a0
10.1021/bi00591a005
10.1146/annurev.bi.58.070189.002321
10.1016/0968-0004(89)90046-7
10.1146/annurev.bi.56.070187.003031
10.1016/S0021-9258(17)42478-1
10.1016/0003-2697(83)90418-9
10.1073/pnas.84.2.388
10.1126/science.3291115
10.1073/pnas.88.4.1115
{'key': 'e_1_2_3_12_1', 'volume-title': 'Antibodies: a laboratory manual', 'author': 'Harlow E.', 'year': '1988'} / Antibodies: a laboratory manual by Harlow E. (1988)
{'key': 'e_1_2_3_13_1', 'first-page': '329', 'volume': '135', 'author': 'Hunter T.', 'year': '1987', 'journal-title': 'NATO Adv. Study inst. Ser.'} / NATO Adv. Study inst. Ser. by Hunter T. (1987)
10.1093/nar/15.20.8125
10.1038/227680a0
10.1093/nar/11.5.1295
10.1101/SQB.1986.051.01.032
10.1038/334661a0
10.1016/S0021-9258(18)91112-9 / J. Biol. Chem. by Novak‐Hofer I. (1984)
10.1016/0014-5793(87)80564-1
10.1016/S0021-9258(18)80075-8
10.1073/pnas.80.14.4218
10.1016/S0021-9258(18)37813-X
{'key': 'e_1_2_3_24_1', 'first-page': '491', 'volume': '229', 'author': 'Saiki R. K.', 'year': '1988', 'journal-title': 'Science'} / Science by Saiki R. K. (1988)
10.1126/science.2999980
{'key': 'e_1_2_3_26_1', 'volume-title': 'Molecular cloning: a laboratory manual', 'author': 'Sambrook J.', 'year': '1989'} / Molecular cloning: a laboratory manual by Sambrook J. (1989)
10.1021/bi00284a025
10.1093/nar/16.15.7583
10.1016/0092-8674(86)90390-9
10.1101/SQB.1980.044.01.079 / Cold Spring Harbour Symp. Quant. Biol. by Vande Woude G. F. (1979)
10.1016/0378-1119(89)90465-4
10.1126/science.3923623
10.1002/j.1460-2075.1990.tb07419.x

Dates

Type	When
Created	20 years, 6 months ago (March 4, 2005, 12:34 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 11:57 a.m.)
Indexed	1 week ago (Aug. 30, 2025, 1:12 p.m.)
Issued	33 years, 11 months ago (Oct. 1, 1991)
Published	33 years, 11 months ago (Oct. 1, 1991)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	33 years, 11 months ago (Oct. 1, 1991)

Funders 0

None

BibTeX

@article{COFFER_1991, title={Molecular cloning and characterisation of a novel putative protein‐serine kinase related to the cAMP‐dependent and protein kinase C families}, volume={201}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1991.tb16305.x}, DOI={10.1111/j.1432-1033.1991.tb16305.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={COFFER, Paul J. and WOODGETT, James R.}, year={1991}, month=oct, pages={475–481} }

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