DOI: 10.1111/j.1432-1033.1991.tb16179.x. GDP‐fucose: β‐<i>N</i>‐acetylglucosamine (Fuc to (Fucα1 → 6GlcNAc)‐Asn‐peptide) α1 → 3‐fucosyltransferase activity in honeybee (<i>Apis mellifica</i>) venom glands

GDP‐fucose: β‐N‐acetylglucosamine (Fuc to (Fucα1 → 6GlcNAc)‐Asn‐peptide) α1 → 3‐fucosyltransferase activity in honeybee (Apis mellifica) venom glands

10.1111/j.1432-1033.1991.tb16179.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Incubation of honeybee (Apis mellifica) venom‐gland extracts with GDP‐[14C]fucose and GlcNAcβ1 → 2Manα1 → 6(GlcNAcβ1 → 2Manα1 → 3)Manβ1 → 4GlcNAcβ1 → 4(Fucα1 → 6)GlcNAcβ1 → N‐Asn‐peptide(NAc) gave a labeled product in 40% yield. Analysis by 500‐MHz 1H‐NMR spectroscopy indicated the transferred fucose (Fuc) residue to be α1 → 3‐linked to the Asn‐bound GlcNAc. Further proof was provided by one‐dimensional and two‐dimensional 1H‐NMR analysis of the incubation mixture, after incubation with β‐N‐acetylhexosaminidase. The established carbohydrate structure image proves the existence of a novel α1 → 3‐fucosyltransferase with the ability to effect difucosylation of the Asn‐bound GlcNAc in N‐glycans.

Bibliography

STAUDACHER, E., ALTMANN, F., GLÃSSL, J., MÃRZ, L., SCHACHTER, H., KAMERLING, J. P., HÃRD, K., & VLIEGENTHART, J. F. G. (1991). GDPâfucose: Î²âNâacetylglucosamine (Fuc to (FucÎ±1 â 6GlcNAc)âAsnâpeptide) Î±1 â 3âfucosyltransferase activity in honeybee (Apis mellifica) venom glands. European Journal of Biochemistry, 199(3), 745â751. Portico.

Authors 8

Erika STAUDACHER (first)
Friedrich ALTMANN (additional)
Josef GLÖSSL (additional)
Leopold MÄRZ (additional)
Harry SCHACHTER (additional)
Johannis P. KAMERLING (additional)
Karl HÅRD (additional)
Johannes F. G. VLIEGENTHART (additional)

References 22 Referenced 46

Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 12:38 a.m.)
Deposited	1 year, 10 months ago (Oct. 1, 2023, 5:44 p.m.)
Indexed	2 weeks, 4 days ago (Aug. 7, 2025, 4:54 a.m.)
Issued	34 years ago (Aug. 1, 1991)
Published	34 years ago (Aug. 1, 1991)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	34 years ago (Aug. 1, 1991)

Funders 0

None

BibTeX

@article{STAUDACHER_1991, title={GDP‐fucose: β‐N‐acetylglucosamine (Fuc to (Fucα1 → 6GlcNAc)‐Asn‐peptide) α1 → 3‐fucosyltransferase activity in honeybee (Apis mellifica) venom glands: The difucosylation of asparagine‐bound N‐acetylglucosamine}, volume={199}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1991.tb16179.x}, DOI={10.1111/j.1432-1033.1991.tb16179.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={STAUDACHER, Erika and ALTMANN, Friedrich and GLÖSSL, Josef and MÄRZ, Leopold and SCHACHTER, Harry and KAMERLING, Johannis P. and HÅRD, Karl and VLIEGENTHART, Johannes F. G.}, year={1991}, month=aug, pages={745–751} }

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