DOI: 10.1111/j.1432-1033.1991.tb16176.x. Purification and characterisation of the insulin‐stimulated protein kinase from rabbit skeletal muscle; close similarity to S6 kinase II

Purification and characterisation of the insulin‐stimulated protein kinase from rabbit skeletal muscle; close similarity to S6 kinase II

10.1111/j.1432-1033.1991.tb16176.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The insulin‐stimulated protein kinase (ISPK) was purified over 50000‐fold from extracts of rabbit skeletal muscle by a procedure involving chromatography on phosphocellulose, fractionation with ammonium sulphate, and further chromatography on DEAE‐cellulose, phenyl‐Superose, Mono S and Mono Q. About 10 μg enzyme was isolated from 800 g muscle (one rabbit) in four days with an overall recovery of 5%. The purified enzyme showed a single protein‐staining band of apparent molecular mass 91 kDa when analysed by SDS/polyacrylamide gel electrophoresis. The ISPK comigrated during SDS/polyacrylamide gel electrophoresis with the enzyme S6 kinase II from Xenopus eggs, and was recognised in immunoblotting and immunoprecipitation experiments by antibodies raised against S6 kinase II. The substrate specificities of ISPK and S6 kinase II were also very similar and like S6 kinase II, ISPK that had been inactivated by protein phosphatase 2A could be reactivated by incubation with mitogen‐activated protein kinase and MgATP. ISPK was distinct from an insulin‐stimulated 70‐kDa S6 kinase from rat liver in both substrate specificity and immunological cross reactivity. It is concluded that ISPK is closely related in structure to S6 kinase II and may be a mammalian equivalent of this enzyme. The possibility that ISPK is involved in mediating a number of the actions of insulin is discussed.

Bibliography

LAVOINNE, A., ERIKSON, E., MALLER, J. L., PRICE, D. J., AVRUCH, J., & COHEN, P. (1991). Purification and characterisation of the insulinâstimulated protein kinase from rabbit skeletal muscle; close similarity to S6 kinase II. European Journal of Biochemistry, 199(3), 723â728. Portico.

Authors 6

Alain LAVOINNE (first)
Eleanor ERIKSON (additional)
James L. MALLER (additional)
Daniel J. PRICE (additional)
Joseph AVRUCH (additional)
Philip COHEN (additional)

References 27 Referenced 89

10.1038/348302a0
10.1073/pnas.82.3.742
10.1016/S0021-9258(17)42478-1
10.1111/j.1432-1033.1989.tb14668.x
10.1111/j.1432-1033.1976.tb10761.x
10.1016/0076-6879(88)59039-0
10.1016/S0021-9258(18)80075-8
10.1016/S0021-9258(19)67779-3 / J. Biol. Chem. by Erikson E. (1991)
10.1016/0014-5793(90)80386-W
10.1016/0003-9861(86)90205-5
10.1073/pnas.83.6.1613
10.1128/MCB.7.9.3147
10.1128/MCB.9.9.3850
10.1016/S0021-9258(18)80057-6 / J. Biol. Chem. by Erikson E. (1989)
10.1073/pnas.87.20.7944
10.1111/j.1432-1033.1981.tb05160.x
10.1016/0003-2697(76)90527-3
10.1038/227680a0
10.1016/S0021-9258(18)91112-9 / J. Biol. Chem. by Novak‐Hofer I. (1984)
10.1073/pnas.85.19.7154
{'key': 'e_1_2_2_22_2', 'first-page': '135', 'volume': '12', 'author': 'Erikson E.', 'year': '1988', 'journal-title': 'Second Mess. Phosphoproteins'} / Second Mess. Phosphoproteins by Erikson E. (1988)
10.1111/j.1432-1033.1989.tb15263.x
10.1038/334715a0
10.1073/pnas.85.10.3377
10.1016/S0021-9258(19)38423-6
10.1016/S0021-9258(18)37813-X
10.1073/pnas.86.18.6940

Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 12:38 a.m.)
Deposited	1 year, 10 months ago (Oct. 1, 2023, 5:44 p.m.)
Indexed	3 months ago (May 20, 2025, 9:31 a.m.)
Issued	34 years ago (Aug. 1, 1991)
Published	34 years ago (Aug. 1, 1991)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	34 years ago (Aug. 1, 1991)

Funders 0

None

BibTeX

@article{LAVOINNE_1991, title={Purification and characterisation of the insulin‐stimulated protein kinase from rabbit skeletal muscle; close similarity to S6 kinase II}, volume={199}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1991.tb16176.x}, DOI={10.1111/j.1432-1033.1991.tb16176.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={LAVOINNE, Alain and ERIKSON, Eleanor and MALLER, James L. and PRICE, Daniel J. and AVRUCH, Joseph and COHEN, Philip}, year={1991}, month=aug, pages={723–728} }

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