DOI: 10.1111/j.1432-1033.1991.tb15781.x. A peptide‐sensitive channel of large conductance is localized on mitochondrial outer membrane

A peptide‐sensitive channel of large conductance is localized on mitochondrial outer membrane

10.1111/j.1432-1033.1991.tb15781.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Applying the technique of ‘tip‐dip’ to mitochondria, we have shown the existence in this organelle of a cationic channel of large conductance, which is blocked by a 13‐residue peptide possessing the sequence of the N‐terminal extremity of the cytochrome c oxidase subunit IV precursor. To study the submitochondrial localization of the channel, the effect of trypsin on isolated channels and on entire mitochondria were compared. One side of isolated channels is sensitive to trypsin, which eliminates the voltage dependence. Channels isolated from trypsinized mitochondria were devoid of voltage dependence and were blocked by the peptide. This suggests a localization of the channel on the outer membrane. Consistent with this hypothesis, the channel was observed with the highest frequency in outer membrane fractions purified by different procedures, either from bovine adrenal cortex or from rat liver mitochondria. Such a localization is also consistent with digitonin solubilization experiments. The channel was solubilized before the inner membrane marker, cytochrome c oxidase. The orientation of the channel was inferred from its trypsin sensitivity and its potential dependence: a transmembrane potential (inside negative) will close the channel.

Bibliography

CHICH, J., GOLDSCHMIDT, D., THIEFFRY, M., & HENRY, J. (1991). A peptideâsensitive channel of large conductance is localized on mitochondrial outer membrane. European Journal of Biochemistry, 196(1), 29â35. Portico.

Authors 4

Jean‐François CHICH (first)
Denise GOLDSCHMIDT (additional)
Michel THIEFFRY (additional)
Jean‐Pierre HENRY (additional)

References 31 Referenced 35

10.1038/279643a0
10.1002/j.1460-2075.1988.tb02962.x
10.1016/0014-5793(87)80555-0
10.1111/j.1432-1033.1989.tb14780.x
10.1016/S0021-9258(19)40136-1
10.1038/330498a0
10.1016/0014-5793(89)81513-3
10.1007/BF01871275
10.1016/S0006-3495(83)84343-4
10.1021/bi00279a003
{'key': 'e_1_2_3_12_2', 'first-page': '479', 'volume-title': 'Physical chemistry of transmembrane ion motion', 'author': 'Wilmsen U.', 'year': '1983'} / Physical chemistry of transmembrane ion motion by Wilmsen U. (1983)
10.1016/0003-2697(61)90006-9
10.1016/0005-2736(71)90123-4
10.1016/0005-2736(85)90238-X
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10.1016/0003-9861(88)90597-8
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10.1038/315503a0
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10.1016/0076-6879(75)42086-9
10.1083/jcb.32.2.415
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10.1016/0014-5793(90)80189-P
{'key': 'e_1_2_3_32_2', 'first-page': '292', 'volume-title': 'Abstracts Fourth European Bioenergetics Conference', 'author': 'Guo X. J.', 'year': '1986'} / Abstracts Fourth European Bioenergetics Conference by Guo X. J. (1986)

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 11:30 p.m.)
Deposited	1 year, 9 months ago (Oct. 31, 2023, 9:46 a.m.)
Indexed	2 months ago (June 27, 2025, 6:35 a.m.)
Issued	34 years, 6 months ago (Feb. 1, 1991)
Published	34 years, 6 months ago (Feb. 1, 1991)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	34 years, 6 months ago (Feb. 1, 1991)

Funders 0

None

BibTeX

@article{CHICH_1991, title={A peptide‐sensitive channel of large conductance is localized on mitochondrial outer membrane}, volume={196}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1991.tb15781.x}, DOI={10.1111/j.1432-1033.1991.tb15781.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={CHICH, Jean‐François and GOLDSCHMIDT, Denise and THIEFFRY, Michel and HENRY, Jean‐Pierre}, year={1991}, month=feb, pages={29–35} }

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