DOI: 10.1111/j.1432-1033.1990.tb19399.x. Molecular cloning of cDNAs for two subunits of rat multicatalytic proteinase

Molecular cloning of cDNAs for two subunits of rat multicatalytic proteinase

10.1111/j.1432-1033.1990.tb19399.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

cDNA clones for two subunits (designated subunits K and L) of rat liver multicatalytic proteinase (MCP) were isolated using oligonucleotide probes synthesized according to their partial amino acid sequences. The encoded polypeptides of subunits K and L consisted of 255 and 261 amino acid residues with calculated molecular mass of 28.3 kDa and 29.5 kDa, respectively. Northern blot analysis revealed that subunits K and L were expressed in all tissues examined and their expression patterns were almost identical. The deduced amino acid sequences showed no similarities to known protein sequences other than the recently reported sequences of rat and Drosophila MCP subunits. Sequence comparison of MCP subunits of rat and Drosophila revealed that the N‐terminal two‐thirds of the sequence (especially the N‐terminal ∼ 20 residues) is conserved, but the C‐terminal third of the sequence shows no similarity, suggesting functional and structural roles for both regions. Implications for the structural and functional aspects of MCP subunits are discussed based on the sequence similarity.

Bibliography

SORIMACHI, H., TSUKAHARA, T., KAWASAKI, H., ISHIURA, S., EMORI, Y., SUGITA, H., & SUZUKI, K. (1990). Molecular cloning of cDNAs for two subunits of rat multicatalytic proteinase. European Journal of Biochemistry, 193(3), 775â781. Portico.

Authors 7

Hiroyuki SORIMACHI (first)
Toshifumi TSUKAHARA (additional)
Hiroshi KAWASAKI (additional)
Shoichi ISHIURA (additional)
Yasufumi EMORI (additional)
Hideo SUGITA (additional)
Koichi SUZUKI (additional)

References 39 Referenced 16

10.1146/annurev.bi.56.070187.002001
10.1016/0003-9861(89)90558-4
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10.1016/0014-5793(85)80854-1
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10.1016/0022-2836(88)90123-4
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10.1093/oxfordjournals.jbchem.a122880
{'key': 'e_1_2_3_25_2', 'first-page': '143', 'volume': '32', 'author': 'Kleinschmidt J. A.', 'year': '1983', 'journal-title': 'Eur. J. Cell Biol.'} / Eur. J. Cell Biol. by Kleinschmidt J. A. (1983)
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10.1002/j.1460-2075.1989.tb08366.x
10.1016/0022-2836(85)90276-1
10.1016/S0021-9258(19)77890-9
10.1111/j.1475-4754.1982.tb01001.x
10.1016/S0021-9258(19)47225-6
10.1016/S0022-2836(83)80284-8
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10.1016/0006-291X(89)91734-8
10.1093/nar/12.2.857
10.1038/263211a0
10.1038/227680a0

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 11:41 p.m.)
Deposited	1 year, 9 months ago (Oct. 31, 2023, 7:02 a.m.)
Indexed	1 year, 9 months ago (Nov. 1, 2023, 3:16 a.m.)
Issued	34 years, 9 months ago (Nov. 1, 1990)
Published	34 years, 9 months ago (Nov. 1, 1990)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	34 years, 9 months ago (Nov. 1, 1990)

Funders 0

None

BibTeX

@article{SORIMACHI_1990, title={Molecular cloning of cDNAs for two subunits of rat multicatalytic proteinase: Existence of N‐terminal conserved and C‐terminal diverged sequences among subunits}, volume={193}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1990.tb19399.x}, DOI={10.1111/j.1432-1033.1990.tb19399.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={SORIMACHI, Hiroyuki and TSUKAHARA, Toshifumi and KAWASAKI, Hiroshi and ISHIURA, Shoichi and EMORI, Yasufumi and SUGITA, Hideo and SUZUKI, Koichi}, year={1990}, month=nov, pages={775–781} }

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