DOI: 10.1111/j.1432-1033.1990.tb19385.x. Okadaic acid activates microtubule‐associated protein kinase in quiescent fibroblastic cells

Okadaic acid activates microtubule‐associated protein kinase in quiescent fibroblastic cells

10.1111/j.1432-1033.1990.tb19385.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Okadaic acid is a potent and specific inhibitor of protein phosphatases 1 and 2A, and is a strong tumor promoter that is not an activator of protein kinase C. Treatment of quiescent cultures of rat fibroblastic 3Y1 cells with okadaic acid induced marked activation of a kinase activity that phosphorylated microtubule‐associated protein (MAP) 2 and myelin basic protein, but not histone or casein, in vitro. This activated kinase eluted at ∼ 0.15 M NaCl on a DEAE‐cellulose column and its apparent molecular mass was determined to be ∼ 40 kDa by gel filtration. Detection of the kinase activity in polyacrylamide gels containing substrate proteins after sodium dodecyl sulfate gel electrophoresis revealed that the okadaic‐acid‐activated kinase activity resided mainly in two closely related polypeptides with apparent molecular mass ∼ 40 kDa. The characteristics of this kinase were indistinguishable from those of the mitogen‐activated MAP kinase in the same cells. The okadaic‐acid‐activated MAP kinase was deactivated by protein phosphatase 2A treatment in vitro. These results suggest that MAP kinase is negatively regulated by protein phosphatases 1 and/or 2A in quiescent cells and therefore can be activated by inhibiting these protein phosphatases. Interestingly, the okadaic‐acid‐induced activation of MAP kinase was transient and epidermal‐growth‐factor‐induced activation was also transient, even in the presence of okadaic acid. These data may imply that protein phosphatases 1 and 2A are not involved in the deactivation of MAP kinase in cells.

Bibliography

GOTOH, Y., NISHIDA, E., & SAKAI, H. (1990). Okadaic acid activates microtubuleâassociated protein kinase in quiescent fibroblastic cells. European Journal of Biochemistry, 193(3), 671â674. Portico.

Authors 3

Yukiko GOTOH (first)
Eisuke NISHIDA (additional)
Hikoichi SAKAI (additional)

References 21 Referenced 59

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 11:41 p.m.)
Deposited	1 year, 10 months ago (Oct. 31, 2023, 7:02 a.m.)
Indexed	1 year, 3 months ago (May 12, 2024, 4:58 p.m.)
Issued	34 years, 9 months ago (Nov. 1, 1990)
Published	34 years, 9 months ago (Nov. 1, 1990)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	34 years, 9 months ago (Nov. 1, 1990)

Funders 0

None

BibTeX

@article{GOTOH_1990, title={Okadaic acid activates microtubule‐associated protein kinase in quiescent fibroblastic cells}, volume={193}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1990.tb19385.x}, DOI={10.1111/j.1432-1033.1990.tb19385.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={GOTOH, Yukiko and NISHIDA, Eisuke and SAKAI, Hikoichi}, year={1990}, month=nov, pages={671–674} }

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