DOI: 10.1111/j.1432-1033.1990.tb15602.x. The two coiled coils in the isolated rod domain of the intermediate filament protein desmin are staggered

The two coiled coils in the isolated rod domain of the intermediate filament protein desmin are staggered

10.1111/j.1432-1033.1990.tb15602.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Desmin protofilaments and the proteolytically derived α‐helical rod domain have been characterized by highresolution gel permeation chromatography (GPC) using columns calibrated for the determination of viscosity radii. Additional characterization by chemical cross‐linking and the determination of sedimentation values allowed the calculation of the molecular dimensions of the molecular species isolated. In dilute buffers GPC separated desmin rod preparations into two complexes: a dimer species (single coiled coil) with a length of 50 ± 5 nm and a tetramer species (two coiled coils) with a length of 65 ± 5 nm. Thus the two coiled coils in the tetramer are staggered by approximately 15 nm. The hydrodynamically derived lengths of the rod dimer and tetramer are supported by electron microscopy after metal shadowing. The hydrodynamic properties of desmin protofilaments follow that of the rod tetramer. The data on the hydrodynamic analysis of the rod tetramer of desmin in solution are in full agreement with the structural information recently deduced from paracrystals of the rod of glial fibrillary acidic protein [Stewart, M., Quinlan, R. A. & Moir, R. D. (1989) J. Cell Biol. 109, 225–234]. Our results explain the inhomogeneity of molecules encountered in previous electron microscopical analyses.

Bibliography

POTSCHKA, M., NAVE, R., WEBER, K., & GEISLER, N. (1990). The two coiled coils in the isolated rod domain of the intermediate filament protein desmin are staggered. European Journal of Biochemistry, 190(3), 503â508. Portico.

Authors 4

Martin POTSCHKA (first)
Rüdiger NAVE (additional)
Klaus WEBER (additional)
Norbert GEISLER (additional)

References 27 Referenced 27

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 11:01 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 3:30 a.m.)
Indexed	1 year, 3 months ago (May 12, 2024, 9:47 p.m.)
Issued	35 years, 3 months ago (June 1, 1990)
Published	35 years, 3 months ago (June 1, 1990)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	35 years, 3 months ago (June 1, 1990)

Funders 0

None

BibTeX

@article{POTSCHKA_1990, title={The two coiled coils in the isolated rod domain of the intermediate filament protein desmin are staggered: A hydrodynamic analysis of tetramers and dimers}, volume={190}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1990.tb15602.x}, DOI={10.1111/j.1432-1033.1990.tb15602.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={POTSCHKA, Martin and NAVE, Rüdiger and WEBER, Klaus and GEISLER, Norbert}, year={1990}, month=jun, pages={503–508} }

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