DOI: 10.1111/j.1432-1033.1990.tb15482.x. Synthetic peptides as model substrates for the study of the specificity of the polycation‐stimulated protein phosphatases

Synthetic peptides as model substrates for the study of the specificity of the polycation‐stimulated protein phosphatases

10.1111/j.1432-1033.1990.tb15482.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The substrate specificity of the different forms of the polycation‐stimulated (PCS, type 2A) protein phosphatases and of the active catalytic subunit of the ATP, Mg‐dependent (type 1) phosphatase (AMDc) was investigated, using synthetic peptides phosphorylated by either cyclic‐AMP‐dependent protein kinase or by casein kinase‐2. The PCS phosphatases are very efficient toward the Thr(P) peptides RRAT(P)VA and RRREEET(P)EEE when compared with the Ser(P) analogues RRAS(P)VA and RRREEES(P)EEEAA. Despite their distinct sequence, both Thr(P) peptides are excellent substrates for the PCSM and PCSH1 Phosphatases, being dephosphorylated faster than phosphorylase a. The slow dephosphorylation of RRAS(P)VA by the PCS phosphatases could be increased substantially by the insertion of N‐terminal (Arg) basic residues. In contrast with the latter, the AMDc phosphatase shows very poor activity toward all the phosphopeptides tested, without preference for either Ser(P) or Thr(P) peptides. However, N‐terminal basic residues also favor the dephosphorylation of otherwise almost inert substrates by the AMDc phosphatase. Hence, while the dephosphorylation of Thr(P) substrates by the PCS phosphatases is highly favored by the nature of the phosphorylated amino acid, phosphatase activity toward Ser(P)‐containing peptides may require specific determinants in the primary structure of the phosphorylation site.

Bibliography

AGOSTINIS, P., GORIS, J., PINNA, L. A., MARCHIORI, F., PERICH, J. W., MEYER, H. E., & MERLEVEDE, W. (1990). Synthetic peptides as model substrates for the study of the specificity of the polycationâstimulated protein phosphatases. European Journal of Biochemistry, 189(2), 235â241. Portico.

Authors 7

Patrizia AGOSTINIS (first)
Jozef GORIS (additional)
Lorenzo A. PINNA (additional)
Fernando MARCHIORI (additional)
John W. PERICH (additional)
Helmut E. MEYER (additional)
Wilfried MERLEVEDE (additional)

References 37 Referenced 61

{'key': 'e_1_2_3_2_2', 'first-page': '1', 'volume': '1', 'author': 'Merlevede W.', 'year': '1985', 'journal-title': 'Adv. Protein Phosphatases'} / Adv. Protein Phosphatases by Merlevede W. (1985)
10.1016/S0021-9258(19)75748-2
{'key': 'e_1_2_3_4_2', 'first-page': '467', 'volume': '2', 'author': 'Waelkens E.', 'year': '1987', 'journal-title': 'Adv. Enzyme Regul.'} / Adv. Enzyme Regul. by Waelkens E. (1987)
10.1042/bj2390109
10.1111/j.1432-1033.1983.tb07357.x
10.1016/S0021-9258(19)75749-4
{'key': 'e_1_2_3_8_2', 'first-page': '327', 'volume': '3', 'author': 'Pinna L. A.', 'year': '1986', 'journal-title': 'Adv. Protein Phosphatases'} / Adv. Protein Phosphatases by Pinna L. A. (1986)
10.1016/S0021-9258(19)77285-8
10.1016/S0021-9258(18)61493-0
10.1016/S0021-9258(18)89514-X / J. Biol. Chem. by Pickett‐Gies C. A. (1985)
10.1016/0065-2571(84)90026-8
10.1016/S0021-9258(18)43212-7
10.1016/0076-6879(74)38046-9
10.1111/j.1432-1033.1983.tb07695.x
10.1111/j.1432-1033.1988.tb14373.x
10.1111/j.1432-1033.1987.tb10894.x
10.1016/S0021-9258(19)77286-X
10.1042/bj2480785
10.1016/0014-5793(77)80279-2
10.1016/0005-2744(78)90014-1
10.1016/S0021-9258(19)70297-X / J. Biol. Chem. by Titanji V. P. K. (1980)
10.1016/0092-8674(80)90327-X
{'key': 'e_1_2_3_24_2', 'first-page': '51', 'volume': '5', 'author': 'Pinna L. A.', 'year': '1989', 'journal-title': 'Adv. Protein Phosphatases'} / Adv. Protein Phosphatases by Pinna L. A. (1989)
10.1016/S0021-9258(19)85658-2
10.1016/0014-5793(86)80056-4
10.1016/S0021-9258(18)48058-1
10.1016/0014-5793(86)80033-3
10.1016/0167-4838(86)90248-7
10.1016/0006-291X(85)91733-4
10.1111/j.1432-1033.1988.tb14203.x
10.1016/0304-4165(88)90182-1
10.1016/0167-4889(88)90006-7
10.1016/0014-5793(88)81296-1
10.1016/S0021-9258(19)77961-7
10.1016/0167-4889(90)90194-I
10.1111/j.1432-1033.1986.tb09962.x
10.1016/0167-4889(88)90149-8

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 11:10 p.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 8:38 p.m.)
Indexed	11 months, 2 weeks ago (Sept. 12, 2024, 3:47 a.m.)
Issued	35 years, 4 months ago (April 1, 1990)
Published	35 years, 4 months ago (April 1, 1990)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	35 years, 4 months ago (April 1, 1990)

Funders 0

None

BibTeX

@article{AGOSTINIS_1990, title={Synthetic peptides as model substrates for the study of the specificity of the polycation‐stimulated protein phosphatases}, volume={189}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1990.tb15482.x}, DOI={10.1111/j.1432-1033.1990.tb15482.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={AGOSTINIS, Patrizia and GORIS, Jozef and PINNA, Lorenzo A. and MARCHIORI, Fernando and PERICH, John W. and MEYER, Helmut E. and MERLEVEDE, Wilfried}, year={1990}, month=apr, pages={235–241} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        9,
        12
      ]
    ],
    "date-time": "2024-09-12T07:47:44Z",
    "timestamp": 1726127264843
  },
  "reference-count": 37,
  "publisher": "Wiley",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            3,
            3
          ]
        ],
        "date-time": "2005-03-03T00:00:00Z",
        "timestamp": 1109808000000
      },
      "content-version": "vor",
      "delay-in-days": 5450,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1990,
        4
      ]
    ]
  },
  "abstract": "<jats:p>The substrate specificity of the different forms of the polycation\u2010stimulated (PCS, type 2A) protein phosphatases and of the active catalytic subunit of the ATP, Mg\u2010dependent (type 1) phosphatase (AMD<jats:sub>c</jats:sub>) was investigated, using synthetic peptides phosphorylated by either cyclic\u2010AMP\u2010dependent protein kinase or by casein kinase\u20102. The PCS phosphatases are very efficient toward the Thr(<jats:italic>P</jats:italic>) peptides RRAT(<jats:italic>P</jats:italic>)VA and RRREEET(<jats:italic>P</jats:italic>)EEE when compared with the Ser(<jats:italic>P</jats:italic>) analogues RRAS(<jats:italic>P</jats:italic>)VA and RRREEES(<jats:italic>P</jats:italic>)EEEAA. Despite their distinct sequence, both Thr(<jats:italic>P</jats:italic>) peptides are excellent substrates for the PCS<jats:sub>M</jats:sub> and PCS<jats:sub>H1</jats:sub> Phosphatases, being dephosphorylated faster than phosphorylase <jats:italic>a</jats:italic>. The slow dephosphorylation of RRAS(<jats:italic>P</jats:italic>)VA by the PCS phosphatases could be increased substantially by the insertion of N\u2010terminal (Arg) basic residues. In contrast with the latter, the AMD<jats:sub>c</jats:sub> phosphatase shows very poor activity toward all the phosphopeptides tested, without preference for either Ser(<jats:italic>P</jats:italic>) or Thr(<jats:italic>P</jats:italic>) peptides. However, N\u2010terminal basic residues also favor the dephosphorylation of otherwise almost inert substrates by the AMD<jats:sub>c</jats:sub> phosphatase. Hence, while the dephosphorylation of Thr(<jats:italic>P</jats:italic>) substrates by the PCS phosphatases is highly favored by the nature of the phosphorylated amino acid, phosphatase activity toward Ser(<jats:italic>P</jats:italic>)\u2010containing peptides may require specific determinants in the primary structure of the phosphorylation site.</jats:p>",
  "DOI": "10.1111/j.1432-1033.1990.tb15482.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        3,
        4
      ]
    ],
    "date-time": "2005-03-04T04:10:12Z",
    "timestamp": 1109909412000
  },
  "page": "235-241",
  "source": "Crossref",
  "is-referenced-by-count": 61,
  "title": "Synthetic peptides as model substrates for the study of the specificity of the polycation\u2010stimulated protein phosphatases",
  "prefix": "10.1111",
  "volume": "189",
  "author": [
    {
      "given": "Patrizia",
      "family": "AGOSTINIS",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Jozef",
      "family": "GORIS",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Lorenzo A.",
      "family": "PINNA",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Fernando",
      "family": "MARCHIORI",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "John W.",
      "family": "PERICH",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Helmut E.",
      "family": "MEYER",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Wilfried",
      "family": "MERLEVEDE",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_3_2_2",
      "first-page": "1",
      "volume": "1",
      "author": "Merlevede W.",
      "year": "1985",
      "journal-title": "Adv. Protein Phosphatases"
    },
    {
      "key": "e_1_2_3_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)75748-2"
    },
    {
      "key": "e_1_2_3_4_2",
      "first-page": "467",
      "volume": "2",
      "author": "Waelkens E.",
      "year": "1987",
      "journal-title": "Adv. Enzyme Regul."
    },
    {
      "key": "e_1_2_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj2390109"
    },
    {
      "key": "e_1_2_3_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1983.tb07357.x"
    },
    {
      "key": "e_1_2_3_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)75749-4"
    },
    {
      "key": "e_1_2_3_8_2",
      "first-page": "327",
      "volume": "3",
      "author": "Pinna L. A.",
      "year": "1986",
      "journal-title": "Adv. Protein Phosphatases"
    },
    {
      "key": "e_1_2_3_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)77285-8"
    },
    {
      "key": "e_1_2_3_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)61493-0"
    },
    {
      "key": "e_1_2_3_11_2",
      "doi-asserted-by": "crossref",
      "first-page": "2046",
      "DOI": "10.1016/S0021-9258(18)89514-X",
      "volume": "260",
      "author": "Pickett\u2010Gies C. A.",
      "year": "1985",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_3_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0065-2571(84)90026-8"
    },
    {
      "key": "e_1_2_3_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)43212-7"
    },
    {
      "key": "e_1_2_3_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0076-6879(74)38046-9"
    },
    {
      "key": "e_1_2_3_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1983.tb07695.x"
    },
    {
      "key": "e_1_2_3_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1988.tb14373.x"
    },
    {
      "key": "e_1_2_3_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1987.tb10894.x"
    },
    {
      "key": "e_1_2_3_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)77286-X"
    },
    {
      "key": "e_1_2_3_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj2480785"
    },
    {
      "key": "e_1_2_3_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(77)80279-2"
    },
    {
      "key": "e_1_2_3_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2744(78)90014-1"
    },
    {
      "key": "e_1_2_3_22_2",
      "doi-asserted-by": "crossref",
      "first-page": "11339",
      "DOI": "10.1016/S0021-9258(19)70297-X",
      "volume": "250",
      "author": "Titanji V. P. K.",
      "year": "1980",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_3_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(80)90327-X"
    },
    {
      "key": "e_1_2_3_24_2",
      "first-page": "51",
      "volume": "5",
      "author": "Pinna L. A.",
      "year": "1989",
      "journal-title": "Adv. Protein Phosphatases"
    },
    {
      "key": "e_1_2_3_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)85658-2"
    },
    {
      "key": "e_1_2_3_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(86)80056-4"
    },
    {
      "key": "e_1_2_3_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)48058-1"
    },
    {
      "key": "e_1_2_3_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(86)80033-3"
    },
    {
      "key": "e_1_2_3_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0167-4838(86)90248-7"
    },
    {
      "key": "e_1_2_3_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(85)91733-4"
    },
    {
      "key": "e_1_2_3_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1988.tb14203.x"
    },
    {
      "key": "e_1_2_3_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-4165(88)90182-1"
    },
    {
      "key": "e_1_2_3_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0167-4889(88)90006-7"
    },
    {
      "key": "e_1_2_3_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(88)81296-1"
    },
    {
      "key": "e_1_2_3_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)77961-7"
    },
    {
      "key": "e_1_2_3_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0167-4889(90)90194-I"
    },
    {
      "key": "e_1_2_3_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1986.tb09962.x"
    },
    {
      "key": "e_1_2_3_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0167-4889(88)90149-8"
    }
  ],
  "container-title": "European Journal of Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1990.tb15482.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1990.tb15482.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T01:38:24Z",
    "timestamp": 1700703504000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1990.tb15482.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1990,
        4
      ]
    ]
  },
  "references-count": 37,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1990,
          4
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1432-1033.1990.tb15482.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1990.tb15482.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1990,
        4
      ]
    ]
  }
}