DOI: 10.1111/j.1432-1033.1990.tb15433.x. Purification and characterization of a cytosolic protein‐tyrosine kinase from porcine spleen

Purification and characterization of a cytosolic protein‐tyrosine kinase from porcine spleen

10.1111/j.1432-1033.1990.tb15433.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

A cytosolic protein‐tyrosine kinase has been highly purified from porcine spleen using [Val5]angiotensin II as a substrate. The purification procedure involves sequential column chromatographies on phosphocellulose, Sephacryl S‐200, casein‐Sepharose 4B, heparin‐Sepharose CL‐6B and anti‐(4‐aminobenzyl phosphonic acid)– Sepharose 4B. Analysis of the most highly purified preparation by SDS/PAGE revealed a major silver‐stained band of molecular mass 40 kDa. The 40‐kDa cytosolic protein‐tyrosine kinase was purified approximately 10000‐fold with an overall yield of about 7%. It had autophosphorylation activity which was carried out by intramolecular catalysis. The stoichiometory of phosphate incorporation was about 1 mol phosphate/mol enzyme. In the autophosphorylation reaction, the apparent Km value for ATP was relatively low, 0.35 μM; Mn2+ was slightly preferred to Mg2+ as divalent cation. [Val5]Angiotensin II phosphorylation activity of the 40‐kDa kinase increased with the amount of phosphate incorporated into the enzyme. A phosphate exchange reaction was observed during the autophosphorylation. These results suggest that the 40‐kDa kinase described here is a different type of protein‐tyrosine kinase than the enzymes so far reported.

Bibliography

KOBAYASHI, T., NAKAMURA, S., TANIGUCHI, T., & YAMAMURA, H. (1990). Purification and characterization of a cytosolic proteinâtyrosine kinase from porcine spleen. European Journal of Biochemistry, 188(3), 535â540. Portico.

Authors 4

Tomoko KOBAYASHI (first)
Shun‐ichi NAKAMURA (additional)
Takanobu TANIGUCHI (additional)
Hirohei YAMAMURA (additional)

References 29 Referenced 38

10.1146/annurev.bi.54.070185.004341
10.1146/annurev.bi.56.070187.004313
10.1146/annurev.bi.57.070188.002303
10.1073/pnas.77.3.1311
10.1016/S0021-9258(17)44462-0
10.1073/pnas.81.8.2347
10.1016/S0021-9258(18)89271-7
10.1073/pnas.80.9.2529
10.1016/0014-5793(84)81386-1
10.1016/S0021-9258(17)39759-4
10.1016/S0021-9258(18)66763-8
10.1111/j.1432-1033.1988.tb14122.x
10.1016/S0006-291X(88)80401-7
10.1177/000456328902600213
10.1073/pnas.79.2.282
10.1038/227680a0
10.1002/j.1460-2075.1984.tb01834.x
10.1016/0003-2697(76)90527-3
10.1016/S0021-9258(17)39565-0 / J. Biol. Chem. by Foulkes J. G. (1985)
10.1016/S0021-9258(18)61547-9
10.1016/S0021-9258(18)68251-1 / J. Biol. Chem. by Kong S. K. (1988)
10.1016/S0021-9258(18)45415-4 / J. Biol. Chem. by Yu G. (1987)
10.1016/0006-291X(89)92827-1
10.1111/j.1432-1033.1988.tb13909.x
10.1016/S0021-9258(18)37409-X
10.1016/S0021-9258(18)68588-6
10.1128/MCB.8.9.3603
10.1073/pnas.86.3.901
10.1016/S0021-9258(18)83201-X

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 10:50 p.m.)
Deposited	1 year, 10 months ago (Oct. 6, 2023, 8:17 p.m.)
Indexed	1 month, 1 week ago (July 20, 2025, 12:31 a.m.)
Issued	35 years, 5 months ago (March 1, 1990)
Published	35 years, 5 months ago (March 1, 1990)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	35 years, 5 months ago (March 1, 1990)

Funders 0

None

BibTeX

@article{KOBAYASHI_1990, title={Purification and characterization of a cytosolic protein‐tyrosine kinase from porcine spleen}, volume={188}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1990.tb15433.x}, DOI={10.1111/j.1432-1033.1990.tb15433.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={KOBAYASHI, Tomoko and NAKAMURA, Shun‐ichi and TANIGUCHI, Takanobu and YAMAMURA, Hirohei}, year={1990}, month=mar, pages={535–540} }

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