DOI: 10.1111/j.1432-1033.1989.tb21083.x. NMR studies of the <i>Escherichia coli trp</i> aporepressor

NMR studies of the Escherichia coli trp aporepressor

10.1111/j.1432-1033.1989.tb21083.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The resonances in the aromatic region of the 1H‐NMR spectrum of the Escherichia coli trp aporepressor have been assigned to amino acid type by two‐dimensional correlated spectroscopy (COSY), homonuclear Hartmann‐Hahn (HOHAHA) spectroscopy and nuclear Overhauser enhancement spectroscopy (NOESY) techniques and studies of the pH dependence of the chemical shifts, in combination with selective deuteration of the protein. Complete sequence‐specific assignments of the aromatic resonances have been made by comparing the observed inter‐residue NOEs with those expected on the basis of the crystal structure of the protein [Zhang, R.‐G., Joachimiak, A., Lawson, C. L., Shevitz, R. W., Otwinowski, Z. & Sigler, P. B. (1987) Nature 327, 591–597]. The latter experiments have also permitted the sequence‐specific assignment of some of the high‐field methyl resonances. The complete assignment of the aromatic region of the spectrum, in particular of resonances from residues at the dimer interface, opens the way to detailed studies of the conformational effects of corepressor and operator binding.

Bibliography

HYDE, E. I., RAMESH, V., ROBERTS, G. C. K., ARROWSMITH, C. H., TREATâCLEMONS, L., KLAIC, B., & JARDETZKY, O. (1989). NMR studies of the Escherichia coli trp aporepressor. European Journal of Biochemistry, 183(3), 545â553. Portico.

Authors 7

Eva I. HYDE (first)
Vasudevan RAMESH (additional)
Gordon C. K. ROBERTS (additional)
Cheryl H. ARROWSMITH (additional)
Lynda TREAT‐CLEMONS (additional)
Branimir KLAIC (additional)
Oleg JARDETZKY (additional)

References 22 Referenced 12

Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 10:21 p.m.)
Deposited	1 year, 10 months ago (Oct. 30, 2023, 10:47 p.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 11:18 a.m.)
Issued	36 years, 1 month ago (Aug. 1, 1989)
Published	36 years, 1 month ago (Aug. 1, 1989)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	36 years, 1 month ago (Aug. 1, 1989)

Funders 0

None

BibTeX

@article{HYDE_1989, title={NMR studies of the Escherichia coli trp aporepressor: Sequence‐specific assignment of the aromatic proton resonances}, volume={183}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1989.tb21083.x}, DOI={10.1111/j.1432-1033.1989.tb21083.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={HYDE, Eva I. and RAMESH, Vasudevan and ROBERTS, Gordon C. K. and ARROWSMITH, Cheryl H. and TREAT‐CLEMONS, Lynda and KLAIC, Branimir and JARDETZKY, Oleg}, year={1989}, month=aug, pages={545–553} }

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