DOI: 10.1111/j.1432-1033.1989.tb14911.x. Interaction of non‐classical detergents with the mitochondrial porin

Interaction of non‐classical detergents with the mitochondrial porin

10.1111/j.1432-1033.1989.tb14911.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The effect of different families of detergents on the solubilization and purification of the pore‐forming protein (porin) of the mitochondrial outer membrane of bovine heart was investigated in detail. With Tritons, dimethylamine oxides and zwittergents, porin solubilization with respect to total mitochondrial membrane protein was more efficient with the more hydrophobic members of each series. With most detergents the protein eluted as protein‐detergent micelles in the void volume of hydroxyapatite/celite columns. In contrast, the protein was bound to the column material and was eluted after the addition of salt to the elution buffer when the detergents octylglucoside, zwittergent Z‐314 and laury(dimethyl)‐amine oxide were used. The protein purified in the presence of the latter detergent had a higher pore‐forming activity in lipid bilayer membranes compared to porin isolated in the presence of Triton X‐100. The binding of porin to the hydroxyapatite/celite column was used to study the lipid content of the active pore‐forming complex. The analysis revealed that the complex contained no phospholipid but rather five molecules of cholesterol/polypeptide chain.

Bibliography

de PINTO, V., BENZ, R., & PALMIERI, F. (1989). Interaction of nonâclassical detergents with the mitochondrial porin. European Journal of Biochemistry, 183(1), 179â187. Portico.

Authors 3

Vito de PINTO (first)
Roland BENZ (additional)
Ferdinando PALMIERI (additional)

References 47 Referenced 90

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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 10:16 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 8:36 a.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 3:08 p.m.)
Issued	36 years, 1 month ago (July 1, 1989)
Published	36 years, 1 month ago (July 1, 1989)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	36 years, 1 month ago (July 1, 1989)

Funders 0

None

BibTeX

@article{de_PINTO_1989, title={Interaction of non‐classical detergents with the mitochondrial porin: A new purification procedure and characterization of the pore‐forming unit}, volume={183}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1989.tb14911.x}, DOI={10.1111/j.1432-1033.1989.tb14911.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={de PINTO, Vito and BENZ, Roland and PALMIERI, Ferdinando}, year={1989}, month=jul, pages={179–187} }

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