DOI: 10.1111/j.1432-1033.1989.tb14832.x. Primary structure of spinach‐chloroplast thioredoxin f

Primary structure of spinach‐chloroplast thioredoxin f

10.1111/j.1432-1033.1989.tb14832.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The primary structure of thioredoxin f from spinach chloroplasts was determined by standard amino acid sequencing and furthermore by sequencing the corresponding nuclear genome region. The protein, with a calculated molecular mass of 12564 Da and a molar absorption coefficient at 280 nm of 17700 M−1 cm−1, consists of 113 residues and exhibits 24% residue identities with spinach chloroplast thioredoxin mb or Escherichia coli thioredoxin.A monospecific antibody elicited against thioredoxin f has been used to select recombinant phage from spinach cDNA libraries in λgt11. The inserts of positive clones were sequenced. They code for a polypeptide of 190 amino acids, composed of the thioredoxin f sequence (113 residues) and an upstream element (77 residues) which most probably consitutes the N‐terminal transit peptide that directs the polypeptide into chloroplasts. In vitro transcription and translation of this construct generates a polypeptide of approximately 21 kDa, which is imported by isolated spinach chloroplasts and processed to the mature 12.5‐kDa protein.

Bibliography

KAMO, M., TSUGITA, A., WIESSNER, C., WEDEL, N., BARTLING, D., HERRMANN, R. G., AGUILAR, F., GARDETâSALVI, L., & SCHÃRMANN, P. (1989). Primary structure of spinachâchloroplast thioredoxin f. European Journal of Biochemistry, 182(2), 315â322. Portico.

Authors 9

Masaharu KAMO (first)
Akira TSUGITA (additional)
Christoph WIESSNER (additional)
Norbert WEDEL (additional)
Dieter BARTLING (additional)
Reinhold G. HERRMANN (additional)
Fernando AGUILAR (additional)
Laura GARDET‐SALVI (additional)
Peter SCHÜRMANN (additional)

References 59 Referenced 58

10.1146/annurev.bi.54.070185.001321
10.1016/0968-0004(79)90191-9
10.1146/annurev.pp.31.060180.002013
10.1016/0304-4173(86)90004-2
10.1007/BF00398677
10.1016/0003-9861(88)90282-2
10.1111/j.1432-1033.1968.tb00470.x
10.1016/S0021-9258(19)52524-8
10.1016/S0021-9258(18)47599-0
10.1111/j.1432-1033.1988.tb13902.x
10.1016/S0021-9258(17)39272-4 / J. Biol. Chem. by Gleason F. K. (1985)
10.1111/j.1432-1033.1984.tb08220.x
10.1111/j.1432-1033.1986.tb09379.x
10.1007/BF01116889
10.1016/0378-1119(84)90015-5
10.1128/jb.163.1.311-316.1985 / J. Bacteriol. by Lim C.‐J. (1985)
{'key': 'e_1_2_3_18_2', 'first-page': '285', 'volume-title': 'Thioredoxin and glutaredoxin systems: structure and function', 'author': 'Tabor S.', 'year': '1986'} / Thioredoxin and glutaredoxin systems: structure and function by Tabor S. (1986)
10.1016/S0021-9258(18)45323-9 / J. Biol. Chem. by Lim C.‐J. (1987)
10.1128/jb.168.3.1258-1264.1986
Alam J.&Curtis S. E.(1986)VIIth Int. Congress on Photosynthesis abstr.308–231 Brown University (USA).
10.1016/S0021-9258(19)84953-0 / J. Biol. Chem. by Muller E. G. D. (1989)
10.1016/S0021-9258(19)81559-4 / J. Biol. Chem. by Jones S. W. (1988)
10.1016/S0021-9258(19)37617-3
10.1016/0006-291X(83)90960-9
{'key': 'e_1_2_3_26_2', 'first-page': '1081', 'volume-title': 'Methods in chloroplast molecular biology', 'author': 'Bartlett S. G.', 'year': '1982'} / Methods in chloroplast molecular biology by Bartlett S. G. (1982)
10.1007/BF02418761
10.1111/j.1432-1033.1981.tb05297.x
10.1016/S0021-9258(18)81308-4
10.1016/0006-3002(58)90016-7
10.1016/S0021-9258(18)43377-7
10.1093/oxfordjournals.jbchem.a122047
10.1007/BF00425507
10.1016/S0021-9258(19)81479-5
10.1073/pnas.74.12.5463
10.1016/S0021-9258(18)89860-X / J. Biol. Chem. by Whittacker M. M. (1984)
10.1515/znc-1986-0306 / Z. Naturforsch. by Langlotz P. (1985)
10.1111/j.1432-1033.1981.tb05635.x
{'key': 'e_1_2_3_39_2', 'first-page': '485', 'volume': '154', 'author': 'Buc J.', 'year': '1984', 'journal-title': 'Eur. J. Biochem.'} / Eur. J. Biochem. by Buc J. (1984)
{'key': 'e_1_2_3_40_2', 'first-page': '31', 'volume-title': 'Thioredoxin and glutaredoxin systems: structure and function', 'author': 'Schürmann P.', 'year': '1986'} / Thioredoxin and glutaredoxin systems: structure and function by Schürmann P. (1986)
10.1021/bi00856a009
10.1111/j.1432-1033.1986.tb10131.x
10.1016/S0021-9258(19)68844-7 / J. Biol. Chem. by Gleason F. K. (1981)
10.1021/bi00514a033
10.1016/S0021-9258(19)45481-1
10.1146/annurev.bi.47.070178.001343
10.1002/j.1460-2075.1984.tb01994.x
10.1016/S0021-9258(18)66820-6
10.1021/bi00405a002
10.1016/0005-2728(81)90082-7
10.1111/j.1432-1033.1985.tb09232.x
{'key': 'e_1_2_3_52_2', 'volume-title': 'Molecular cloning: a laboratory manual', 'author': 'Maniatis T.', 'year': '1982'} / Molecular cloning: a laboratory manual by Maniatis T. (1982)
10.1111/j.1432-1033.1989.tb14679.x
10.1007/BF02427758
10.1016/0022-2836(86)90394-3
10.1007/BF00331591
10.1016/0014-5793(87)80696-8
10.1016/0022-2836(82)90515-0
10.1007/BF00365689
10.1016/S0076-6879(72)25041-8

Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 10:12 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 5:58 p.m.)
Indexed	1 month ago (Aug. 6, 2025, 9:19 a.m.)
Issued	36 years, 3 months ago (June 1, 1989)
Published	36 years, 3 months ago (June 1, 1989)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	36 years, 3 months ago (June 1, 1989)

Funders 0

None

BibTeX

@article{KAMO_1989, title={Primary structure of spinach‐chloroplast thioredoxin f: Protein sequencing and analysis of complete cDNA clones for spinach‐chloroplast thioredoxin f}, volume={182}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1989.tb14832.x}, DOI={10.1111/j.1432-1033.1989.tb14832.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={KAMO, Masaharu and TSUGITA, Akira and WIESSNER, Christoph and WEDEL, Norbert and BARTLING, Dieter and HERRMANN, Reinhold G. and AGUILAR, Fernando and GARDET‐SALVI, Laura and SCHÜRMANN, Peter}, year={1989}, month=jun, pages={315–322} }

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