DOI: 10.1111/j.1432-1033.1988.tb14342.x. Structural study of long arm fragments of laminin

Structural study of long arm fragments of laminin

10.1111/j.1432-1033.1988.tb14342.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The outer segments of the long arm of laminin have recently been shown to mediate attachment of many cell types and to stimulate neurite outgrowth. For a structural characterization of this part of the molecule we prepared, by limited elastase digestion of laminin, fragments E3 and E8, previously identified as a globular heparin‐binding domain and as a 35‐nm‐long rod with a terminal globule, respectively. Fragment E3 is a domain adjacent to fragment E8. Both structures together comprise the complete terminal half of the long arm. Our data confirm current models, which predict that the C‐terminal segments from all three chains contribute to its structure. The B chains terminate at the end of the rod like domain, while the large terminal globule is formed by A‐chain structures only. In addition to fragment E3, two new fragments T1 and T2 obtained by tryptic cleavage of fragment E8 were characterized as substructures of the globular domain. Screening of a mouse cDNA library with synthetic oligonucleotides allowed isolation of an 1.8‐kb cDNA clone encoding 547 C‐terminal amino acids of the A chain and some 196 nucleotides of the 3′‐untranslated region including a single polyadenylation site. The clone contained portions of domain T2 and the complete heparin binding domain E3 which was thus identified as the most C‐terminal domain of the A chain. Sequence alignment indicated that the terminal globule is formed by homologous repeats of some 140 residues having no counterpart in the B chains.

Bibliography

DEUTZMANN, R., HUBEP, J., SCHMETZ, K. A., OBERBÃMER, I., & HARTL, L. (1988). Structural study of long arm fragments of laminin. European Journal of Biochemistry, 177(1), 35â45. Portico.

Authors 5

Rainer DEUTZMANN (first)
Johann HUBEP (additional)
Konrad A. SCHMETZ (additional)
Ilse OBERBÄMER (additional)
Lydia HARTL (additional)

References 44 Referenced 66

10.1016/S0021-9258(19)83607-4
10.1016/0022-2836(81)90326-0
10.1073/pnas.84.4.935
10.1016/S0021-9258(18)45498-1
10.1111/j.1432-1033.1988.tb14045.x
10.1002/jcb.240270402
10.1016/S0232-1513(86)80002-0
10.1146/annurev.cb.03.110187.000421
10.1016/0006-291X(85)91642-0
10.1016/0022-2836(86)90391-8
10.1083/jcb.100.6.1848
10.1111/j.1432-1033.1982.tb06499.x
10.1016/S0021-9258(18)32145-8
10.1083/jcb.103.5.1689
10.1016/S0021-9258(17)39656-4
10.1172/JCI111501
10.1083/jcb.105.1.589
10.1016/S0021-9258(18)60840-3
10.1002/j.1460-2075.1984.tb01997.x
10.1083/jcb.103.6.2457
10.1016/S0021-9258(18)60982-2
{'key': 'e_1_2_3_23_2', 'author': 'Pikkarainen T.', 'year': '1988', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. by Pikkarainen T. (1988)
10.1016/0092-8674(87)90707-0
10.1002/j.1460-2075.1984.tb02140.x
10.1002/j.1460-2075.1985.tb03630.x
10.1111/j.1432-1033.1987.tb13476.x
10.1016/0076-6879(87)38030-9
10.1515/bchm2.1980.361.2.1829
10.1002/jcp.1040840103
10.1073/pnas.74.8.3399
10.1016/S0040-4039(01)90461-7
10.1073/pnas.74.11.4835
10.1093/nar/8.2.299
10.1073/pnas.82.6.1585
10.1016/0378-1119(86)90387-2
10.1093/nar/7.6.1513
10.1016/0378-1119(85)90120-9
10.1089/dna.1985.4.165
10.1016/S0021-9258(18)34135-8
10.1016/0092-8674(82)90024-1 / Cell by Alonso S. (1982)
10.1038/303821a0
10.1073/pnas.82.11.3771
10.1093/nar/15.22.9499
10.1021/bi00627a029

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 9:28 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 9:37 a.m.)
Indexed	1 year ago (July 28, 2024, 3:15 a.m.)
Issued	36 years, 10 months ago (Oct. 1, 1988)
Published	36 years, 10 months ago (Oct. 1, 1988)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	36 years, 10 months ago (Oct. 1, 1988)

Funders 0

None

BibTeX

@article{DEUTZMANN_1988, title={Structural study of long arm fragments of laminin: Evidence for repetitive C‐terminal sequences in the A‐chain, not present in the B‐chains}, volume={177}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1988.tb14342.x}, DOI={10.1111/j.1432-1033.1988.tb14342.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={DEUTZMANN, Rainer and HUBEP, Johann and SCHMETZ, Konrad A. and OBERBÄMER, Ilse and HARTL, Lydia}, year={1988}, month=oct, pages={35–45} }

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