DOI: 10.1111/j.1432-1033.1988.tb14308.x. A new siliconized‐glass fiber as support for protein‐chemical analysis of electroblotted proteins.

A new siliconized‐glass fiber as support for protein‐chemical analysis of electroblotted proteins.

10.1111/j.1432-1033.1988.tb14308.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

A new hydrophobic glass‐fiber support is presented, which is well suited to the electrophoretic transfer of proteins from polyacrylamide gels and subsequent protein‐chemical analysis. Modified glass‐fiber sheets are easily prepared by chemical reaction of the surface with poly(methyl‐3,3,3‐trifluoropropylsiloxane) in trifluoroacetic acid. The modification is stable during electroblotting, amino acid sequence analysis and hydrolysis. The siliconized glass fiber exhibits a high protein‐binding capacity, allows the application of well‐established staining procedures, and does not interfere with the analytical methods of modern protein chemistry at the low picomole level. Samples separated by electrophoresis and immobilized on hydrophobic supports fail to exhibit any detectable contamination in amino acid sequence analysis hence allowing the high performance of the available protein‐chemical methods to be exploited.

Bibliography

ECKERSKORN, C., MEWES, W., GORETZKI, H., & LOTTSPEICH, F. (1988). A new siliconizedâglass fiber as support for proteinâchemical analysis of electroblotted proteins. European Journal of Biochemistry, 176(3), 509â519. Portico.

Authors 4

Christoph ECKERSKORN (first)
Werner MEWES (additional)
Hans GORETZKI (additional)
Friedrich LOTTSPEICH (additional)

References 21 Referenced 248

10.1016/S0021-9258(17)35652-1
10.1111/j.1432-1033.1985.tb09157.x
10.1016/S0021-9258(18)61070-1
10.1038/227680a0
10.1073/pnas.76.9.4350 / Proc. Natl Acad. Sci. USA by Towbin H. (1979)
10.1093/oxfordjournals.jbchem.a122209
10.1016/S0021-9673(01)89028-2
10.1016/S0021-9673(01)87457-4
10.1007/978-1-4757-0118-0
10.1080/10408347508085581
10.1002/sia.740030506
10.1016/0165-022X(84)90040-X
10.1007/BF00959526
10.1016/0014-5793(87)81160-2
10.1016/0005-2728(88)90186-7
Marget M. Eckardt A. Ehret W. Specht V. U. Duchene M.&Domdey(1988)Gene (Amst.) in the press.
10.1016/0168-1702(88)90014-7
10.1111/j.1432-1033.1987.tb13377.x / Eur. J. Biochem. by Shiozawa J. A. (1987)
10.1002/eji.1830180605
10.1126/science.3285470
Barker W. C. Hunt L. T. George D. G. Yeh L. S. Chenn H. R. Blomquist M. C. Seibel‐Ross E. I. Elzanowski A. Blair J. K. Ferrick D. A. Hong.M. K.&Cedley R. S.(1987)National Biomedical Research Foundation Release 13.

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 9:21 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 6:48 a.m.)
Indexed	3 weeks, 1 day ago (Aug. 7, 2025, 4:54 a.m.)
Issued	36 years, 10 months ago (Oct. 1, 1988)
Published	36 years, 10 months ago (Oct. 1, 1988)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	36 years, 10 months ago (Oct. 1, 1988)

Funders 0

None

BibTeX

@article{ECKERSKORN_1988, title={A new siliconized‐glass fiber as support for protein‐chemical analysis of electroblotted proteins.}, volume={176}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1988.tb14308.x}, DOI={10.1111/j.1432-1033.1988.tb14308.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={ECKERSKORN, Christoph and MEWES, Werner and GORETZKI, Hans and LOTTSPEICH, Friedrich}, year={1988}, month=oct, pages={509–519} }

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