Tertiary structure of Escherichia coli tRNAThr3 in solution and interaction of this tRNA with the cognate threonyl‐tRNA synthetase
10.1111/j.1432-1033.1988.tb14223.x
Crossref journal-article
Wiley
European Journal of Biochemistry (311)
Abstract

The solution structure of Escherichia coli tRNAThr3 (anticodon GGU) and the residues of this tRNA in contact with the α2 dimeric threonyl‐tRNA synthetase were studied by chemical and enzymatic footprinting experiments. Alkylation of phosphodiester bonds by ethylnitrosourea and of N‐7 positions in guanosines and N‐3 positions in cytidines by dimethyl sulphate as well as carbethoxylation of N‐7 positions in adenosines by diethyl pyrocarbonate were conducted on different conformers of tRNAThr3. The enzymatic structural probes were nuclease S1 and the cobra venom ribonuclease. Results will be compared to those of three other tRNAs, tRNAAsp, tRNAPhe and tRNATrp, already mapped with these probes.The reactivity of phosphates towards ethylnitrosourea of the unfolded tRNA was compared to that of the native molecule. The alkylation pattern of tRNAThr3 shows some similarities to that of yeast tRNAPhe and mammalian tRNATrp, especially in the D‐arm (positions 19 and 24) and with tRNATrp, at position 50, the junction between the variable region and the T‐stem. In the T‐loop, tRNAThr3, similarly to the three other tRNAs, shows protections against alkylation at phosphates 59 and 60. However, tRNAThr3 is unique as far as very strong protections are also found for phosphates 55 to 58 in the T‐loop. Compared with yeast tRNAAsp, the main differences in reactivity concern phosphates 19, 24 and 50.Mapping of bases with dimethyl sulphate and diethyl pyrocarbonate reveal conformational similarities with yeast tRNAPhe. A striking conformational feature of tRNAThr3 is found in the 3′‐side of its anticodon stem, where G40, surrounded by two G residues, is alkylated under native conditions, in contrast to other G residues in stem regions of tRNAs which are unreactive when sandwiched between two purines. This data is indicative of a perturbed helical conformation in the anticodon stem at the level of the 30–40 base pairs.Footprinting experiments, with chemical and enzymatic probes, on the tRNA complexed with its cognate threonyl‐tRNA synthetase indicate significant protections in the anticodon stem and loop region, in the extra‐loop, and in the amino acid accepting region. The involvement of the anticodon of tRNAThr3 in the recognition process with threonyl‐tRNA synthetase was demonstrated by nuclease S1 mapping and by the protection of G34 and G35 against alkylation by dimethyl sulphate. These data are discussed in the light of the tRNA/synthetase recognition problem and of the structural and functional properties of the tRNA‐like structure present in the operator region of the thrSmRNA.

Bibliography

THEOBALD, A., SPRINGER, M., GRUNBERG‐MANAGO, M., EBEL, J., & GIEGE, R. (1988). Tertiary structure of Escherichia coli tRNAThr3 in solution and interaction of this tRNA with the cognate threonyl‐tRNA synthetase. European Journal of Biochemistry, 175(3), 511–524. Portico.

Authors 5
  1. Anne THEOBALD (first)
  2. Mathias SPRINGER (additional)
  3. Marianne GRUNBERG‐MANAGO (additional)
  4. Jean‐Pierre EBEL (additional)
  5. Richard GIEGE (additional)
References 63 Referenced 33
  1. 10.1146/annurev.bi.45.070176.004105
  2. 10.1016/S0079-6603(08)60350-5
  3. 10.1016/0022-2836(87)90240-3
  4. 10.1111/j.1432-1033.1983.tb07395.x
  5. 10.1016/0022-2836(85)90294-3
  6. 10.1016/0022-2836(86)90427-4
  7. 10.1016/0022-2836(87)90336-6
  8. 10.1016/0022-2836(75)90214-4
  9. 10.1073/pnas.77.8.4679
  10. 10.1002/bip.360220410
  11. 10.1073/pnas.72.12.4866
  12. 10.1016/S0022-2836(76)80109-X
  13. 10.1016/0022-2836(78)90209-7
  14. 10.1107/S0567740878006056 / Acta Crystallogr. by Stout C. D. (1978)
  15. 10.1038/288669a0
  16. 10.1016/0022-2836(85)90048-8
  17. 10.1111/j.1432-1033.1984.tb07882.x
  18. 10.1093/nar/12.5.2259
  19. 10.1002/j.1460-2075.1982.tb01158.x
  20. 10.1016/S0021-9258(19)42139-X
  21. 10.1016/0378-1119(80)90013-X
  22. 10.1073/pnas.80.20.6152
  23. 10.1016/S0021-9258(17)42980-2 / J. Biol. Chem. by Lestienne P. (1984)
  24. 10.1016/0022-2836(85)90185-8
  25. {'key': 'e_1_2_3_26_2', 'first-page': '463', 'volume-title': 'NATO ASI Series, Series H: Cell Biology', 'author': 'Springer M.', 'year': '1987'} / NATO ASI Series, Series H: Cell Biology by Springer M. (1987)
  26. 10.1073/pnas.83.12.4384
  27. 10.1016/S0300-9084(77)80050-3 / Biochimie (Paris) by Kern D.
  28. 10.1016/0022-0248(86)90172-7
  29. 10.1016/0006-291X(83)91569-3
  30. {'key': 'e_1_2_3_31_2', 'first-page': '81', 'volume': '50', 'author': 'Rether B.', 'year': '1974', 'journal-title': 'Eur. J. Biochem.'} / Eur. J. Biochem. by Rether B. (1974)
  31. 10.1073/pnas.76.4.1760
  32. 10.1111/j.1432-1033.1981.tb05575.x / Eur. J. Biochem. by Vlassov V. V. (1981)
  33. 10.1016/S0300-9084(80)80285-9 / Biochimie (Paris) by Kern D.
  34. 10.1016/S0021-9258(18)91926-5
  35. 10.1093/nar/4.12.4091
  36. 10.1073/pnas.74.2.560
  37. 10.1016/0014-5793(78)80145-8
  38. 10.1038/269833a0
  39. 10.1007/978-94-009-7225-4_31
  40. 10.1107/S0021889878013308
  41. 10.1111/j.1432-1033.1975.tb02364.x
  42. 10.1016/0022-2836(79)90508-4
  43. 10.1016/0014-5793(81)80307-9
  44. 10.1080/07391102.1987.10506389
  45. 10.1093/nar/15.suppl.r53
  46. 10.1021/bi00507a055
  47. 10.1016/0003-9861(80)90044-2
  48. {'key': 'e_1_2_3_49_2', 'first-page': '11', 'volume-title': 'Chemicals and DNA', 'author': 'Margison G. P.', 'year': '1978'} / Chemicals and DNA by Margison G. P. (1978)
  49. 10.1016/0014-5793(83)80871-0
  50. {'key': 'e_1_2_3_51_2', 'first-page': '578', 'volume': '40', 'author': 'Vassilenko S. K.', 'year': '1975', 'journal-title': 'Biokhimiya'} / Biokhimiya by Vassilenko S. K. (1975)
  51. 10.1016/S0300-9084(82)80264-2 / Biochimie (Paris) by Dessen P. (1981)
  52. 10.1016/S0300-9084(82)80440-9
  53. 10.1080/07391102.1987.10506419
  54. 10.1021/bi00654a014
  55. 10.1111/j.1432-1033.1972.tb02513.x
  56. 10.1080/07391102.1983.10507435
  57. 10.1093/nar/4.5.1649
  58. 10.1016/0014-5793(69)80155-9
  59. 10.1021/bi00638a020
  60. 10.1016/S0300-9084(73)80415-8
  61. {'key': 'e_1_2_3_62_2', 'first-page': '325', 'volume-title': 'Transfer RNA; structure, properties and recognition', 'author': 'Ebel J. P.', 'year': '1980'} / Transfer RNA; structure, properties and recognition by Ebel J. P. (1980)
  62. Moine H. Romby P. Springer M. Grunberg‐Manago M. Ebel J. P. Ehresmann C.&Ehresmann B.(1988)Proc. Natl Acad. Sci. USA in the press.
  63. 10.1093/nar/8.17.3793
Dates
Type When
Created 20 years, 6 months ago (March 3, 2005, 9:21 p.m.)
Deposited 1 year, 9 months ago (Nov. 22, 2023, 9:45 p.m.)
Indexed 1 day, 17 hours ago (Sept. 4, 2025, 9:46 a.m.)
Issued 37 years, 1 month ago (Aug. 1, 1988)
Published 37 years, 1 month ago (Aug. 1, 1988)
Published Online 20 years, 6 months ago (March 3, 2005)
Published Print 37 years, 1 month ago (Aug. 1, 1988)
Funders 0

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@article{THEOBALD_1988, title={Tertiary structure of Escherichia coli tRNAThr3 in solution and interaction of this tRNA with the cognate threonyl‐tRNA synthetase}, volume={175}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1988.tb14223.x}, DOI={10.1111/j.1432-1033.1988.tb14223.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={THEOBALD, Anne and SPRINGER, Mathias and GRUNBERG‐MANAGO, Marianne and EBEL, Jean‐Pierre and GIEGE, Richard}, year={1988}, month=aug, pages={511–524} }