DOI: 10.1111/j.1432-1033.1988.tb13961.x. Modulation of the substrate specificity of the polycation‐stimulated protein phosphatase from <i>Xenopus laevis</i> oocytes

Modulation of the substrate specificity of the polycation‐stimulated protein phosphatase from Xenopus laevis oocytes

10.1111/j.1432-1033.1988.tb13961.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

A polycation‐stimulated (PCS) protein phosphatase was isolated in high yield (280 μg/100 g ovaries) from Xenopus laevis oocytes through a procedure involving a tyrosine‐agarose hydrophobic chromatography. The 220‐kDa enzyme contains a 35‐kDa and a 62‐kDa subunit. It was identified as the low‐Mr polycation‐stimulated (PCSL) protein phosphatase. The labile p‐nitrophenyl phosphatase activity, copurifying with the phosphorylase phosphatase activity, can be increased severalfold by preincubating the purified enzyme with ATP, its analogues or PPi. This activation is time‐dependent and accompanied by a parallel decrease of the phosphorylase phosphatase activity. Although the stimulation was antagonized by metal ions during the preincubation, the basal and ATP‐stimulated p‐nitrophenyl phosphatase requires Mg2+ or Mn2+ in the assay, with pH optima of 8.5–9 and 7.5 respectively.

Bibliography

HERMANN, J., CAYLA, X., DUMORTIER, K., GORIS, J., OZON, R., & MERLEVEDE, W. (1988). Modulation of the substrate specificity of the polycationâstimulated protein phosphatase from Xenopus laevis oocytes. European Journal of Biochemistry, 173(1), 17â25. Portico.

Authors 6

Jacques HERMANN (first)
Xavier CAYLA (additional)
Kathelijn DUMORTIER (additional)
Jozef GORIS (additional)
René OZON (additional)
Wilfried MERLEVEDE (additional)

References 49 Referenced 27

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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 9:08 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 6:22 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 6:40 p.m.)
Issued	37 years, 4 months ago (April 1, 1988)
Published	37 years, 4 months ago (April 1, 1988)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	37 years, 4 months ago (April 1, 1988)

Funders 0

None

BibTeX

@article{HERMANN_1988, title={Modulation of the substrate specificity of the polycation‐stimulated protein phosphatase from Xenopus laevis oocytes}, volume={173}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1988.tb13961.x}, DOI={10.1111/j.1432-1033.1988.tb13961.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={HERMANN, Jacques and CAYLA, Xavier and DUMORTIER, Kathelijn and GORIS, Jozef and OZON, René and MERLEVEDE, Wilfried}, year={1988}, month=apr, pages={17–25} }

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