DOI: 10.1111/j.1432-1033.1987.tb13700.x. The function of the human factor V carbohydrate moiety in blood coagulation

The function of the human factor V carbohydrate moiety in blood coagulation

10.1111/j.1432-1033.1987.tb13700.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Human factor V was subjected to desialation and deglycosylation to investigate the function of the molecular carbohydrate moiety. Removal of 90% of the sialic acid residues resulted in a 1.5–2‐fold increase in clotting activity, and up to 70% deglycosylation in a concurrent decrease in clotting activity. Desialation had no effect on thrombin‐induced activation, whereas deglycosylated factor V activation was impaired. Lectin‐blot experiments with sialic‐acid‐specific Limax flavus agglutinin (LFA), galactose‐specific Ricinus communis agglutinin (RCA‐II) and mannose‐specific concanavalin A on thrombin‐induced factor V fragments revealed the presence of carbohydrate residues in fragments B, C1, D and F1F2. Interestingly, sialic acid was present in C1 whilst galactose was not detectable. Fragment F1 F2 contained terminal galactose residues. LFA and RCA‐II inhibited the procoagulant activity of native factor V and of desialated factor V respectively. These investigations distinctly indicate the important role of the human factor V carbohydrate moiety in the process of blood coagulation.

Bibliography

BRUIN, T., STURK, A., TEN CATE, J. W., & CATH, M. (1987). The function of the human factor V carbohydrate moiety in blood coagulation. European Journal of Biochemistry, 170(1â2), 305â310. Portico.

Authors 4

Taco BRUIN (first)
Augueste STURK (additional)
Jan W. TEN CATE (additional)
Marieke CATH (additional)

References 22 Referenced 13

10.1016/S0021-9258(20)65178-X
10.1016/0003-9861(75)90342-2
10.1016/S0021-9258(18)69851-5
10.1021/ac60111a017
10.3181/00379727-84-20738
10.1038/227680a0
10.1073/pnas.76.9.4350
10.1016/S0021-9258(19)70079-9 / J. Biol. Chem. by Kane W. H. (1981)
10.1016/0167-4838(83)90036-5 / Biochim. Biophys. Acta by Bruin T. (1983)
10.1016/0003-2697(81)90703-X
10.1172/JCI111628
10.1073/pnas.79.15.4540
10.1016/0304-4165(71)90197-8 / Biochim. Biophys. Acta by Skaung K. (1971)
10.1038/236147a0
10.1016/S0021-9258(19)86586-9 / J. Biol. Chem. by Sodetz S. J. M. (1979)
10.1016/0049-3848(82)90057-3
10.1016/0005-2744(81)90015-2 / Biochim. Biophys. Acta by Nishide H. (1981)
10.1021/bi00264a022
10.1021/bi00279a019
10.1016/S0021-9258(18)50383-5
10.1016/S0021-9258(18)32366-4 / J. Biol. Chem. by Mizuochi T. (1983)
10.1016/S0021-9258(19)76994-4 / J. Biol. Chem. by Morell A. G. (1970)

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 8:52 p.m.)
Deposited	1 year, 11 months ago (Aug. 30, 2023, 3:51 p.m.)
Indexed	1 year, 9 months ago (Oct. 28, 2023, 1:46 a.m.)
Issued	37 years, 8 months ago (Dec. 1, 1987)
Published	37 years, 8 months ago (Dec. 1, 1987)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	37 years, 8 months ago (Dec. 1, 1987)

Funders 0

None

BibTeX

@article{BRUIN_1987, title={The function of the human factor V carbohydrate moiety in blood coagulation}, volume={170}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1987.tb13700.x}, DOI={10.1111/j.1432-1033.1987.tb13700.x}, number={1–2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={BRUIN, Taco and STURK, Augueste and TEN CATE, Jan W. and CATH, Marieke}, year={1987}, month=dec, pages={305–310} }

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