DOI: 10.1111/j.1432-1033.1987.tb13414.x. Two hydrophobic low‐molecular‐mass protein fractions of pulmonary surfactant

Two hydrophobic low‐molecular‐mass protein fractions of pulmonary surfactant

10.1111/j.1432-1033.1987.tb13414.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Hydrophobic low‐molecular‐mass proteins were isolated from minced pig lungs and separated into two fractions. Electrophoresis of protein fraction 1 showed two major bands. Calculations of molecular masses from the electrophoretic mobilities are unreliable because of the extreme hydrophobicity of the peptides. However, the two bands were at positions corresponding to apparent molecular masses of about 3 kDa and 14 kDa, while sequence degradation disclosed only one major structure. Electrophoretic separation of protein fraction 2 revealed one band, at an apparent molecular mass of about 6 kDa. Microheterogeneities at the N terminus of both fractions were observed. However, the two fractions had different N‐terminal structures and amino acid compositions. Consequently they are concluded to represent different polypeptides without common segments. Bronchoalveolar lavage from humans also contains surfactant polypeptides and at least the fraction 2 peptide is highly similar in human and porcine surfactants.Artificial surfactant preparations, obtained by recombination of protein fraction 1 or 2 with a mixture of synthetic phospholipids, were evaluated with the pulsating bubble method and in experiments on artificially ventilated premature newborn rabbits. The addition of protein fraction 1 to the phospholipid mixture improved surface adsorption from more than 300 s to about 2 s and reduced minimum surface tension from more than 20 mN/m to nearly 0 as measured with a pulsating bubble. When this surfactant preparation was instilled into the airways of newborn rabbits, the tidal volumes at insufflation pressure 25 cm H2O was increased about twentyfold compared to the volumes obtained in non‐treated controls. Preparations based on protein fraction 1 had better in vitro and in vivo properties than those based on protein fraction 2. Both these protein‐based preparations were decidedly more effective than phospholipids alone.

Bibliography

CURSTEDT, T., JÃRNVALL, H., ROBERTSON, B., BERGMAN, T., & BERGGREN, P. (1987). Two hydrophobic lowâmolecularâmass protein fractions of pulmonary surfactant. European Journal of Biochemistry, 168(2), 255â262. Portico.

Authors 5

Tore CURSTEDT (first)
Hans JÖRNVALL (additional)
Bengt ROBERTSON (additional)
Tomas BERGMAN (additional)
Per BERGGREN (additional)

References 43 Referenced 209

10.1152/ajplegacy.1973.224.4.788
10.1042/bj1830731
{'key': 'e_1_2_2_4_2', 'first-page': '562', 'volume': '40', 'author': 'Katyal S. L.', 'year': '1979', 'journal-title': 'Lab. Invest.'} / Lab. Invest. by Katyal S. L. (1979)
{'key': 'e_1_2_2_5_2', 'first-page': '408', 'volume': '40', 'author': 'Claypool W. D.', 'year': '1981', 'journal-title': 'Fed. Proc.'} / Fed. Proc. by Claypool W. D. (1981)
10.1016/0005-2760(81)90257-5
10.1016/0006-291X(86)90026-4
10.1203/00006450-198605000-00016
10.1203/00006450-198608000-00009
10.1042/bj2360085
10.1159/000410001 / Prog. Respir. Res. by Benson B. J. (1984)
10.1016/0005-2760(84)90048-1
10.1073/pnas.82.19.6379
10.1038/317361a0
10.1016/0005-2736(79)90092-0
{'key': 'e_1_2_2_16_2', 'first-page': '1258', 'volume': '134', 'author': 'Revak S. D.', 'year': '1986', 'journal-title': 'Am. Rev. Respir. Dis.'} / Am. Rev. Respir. Dis. by Revak S. D. (1986)
10.1152/jappl.1980.49.1.34
10.3109/01902148509069678
10.1016/S0022-2275(20)38173-6
{'key': 'e_1_2_2_20_2', 'first-page': '336', 'volume': '69', 'author': 'Suzuki Y.', 'year': '1986', 'journal-title': 'Eur. J. Respir. Dis.'} / Eur. J. Respir. Dis. by Suzuki Y. (1986)
10.1172/JCI111483
10.1016/S0022-2275(20)35198-1
10.1016/0005-2760(74)90251-3
Noack G. Berggren P. Curstedt T. Grossmann G. Herin P. Mortensson W. Nilsson R.&Robertson B.(1987)Acta Pediatr. Scand. in the press.
{'key': 'e_1_2_2_25_2', 'first-page': '116', 'volume-title': 'Hyaline membrane disease – surfactant and its pharmacological influence', 'author': 'Robertson B.', 'year': '1987'} / Hyaline membrane disease – surfactant and its pharmacological influence by Robertson B. (1987)
10.1016/S0378-4347(00)80353-9
10.1016/0003-2697(71)90436-2
10.1016/S0021-9258(18)70226-3
10.1016/S0022-2275(20)40190-7
{'key': 'e_1_2_2_30_2', 'volume-title': 'Methods in protein sequence analysis', 'author': 'Bergman T.', 'year': '1987'} / Methods in protein sequence analysis by Bergman T. (1987)
10.1016/0003-2697(77)90276-7
10.1152/jappl.1977.43.2.198
10.1111/j.1399-6576.1982.tb01733.x
10.1203/00006450-197804000-00001
Robertson B.&Lachmann B.(1987)Exp. Lung Res. in the press.
10.1136/adc.55.10.758
{'key': 'e_1_2_2_37_2', 'first-page': '125', 'volume': '15', 'author': 'Kobayashi T.', 'year': '1984', 'journal-title': 'J. Jpn Med. Soc. Biol. Interface'} / J. Jpn Med. Soc. Biol. Interface by Kobayashi T. (1984)
10.1007/BF00451913
10.1016/S0022-3476(85)80416-9
10.1152/jappl.1983.55.3.875 / J. Appl. Physiol. by Egan E. A. (1983)
{'key': 'e_1_2_2_41_2', 'volume-title': 'Methods in protein sequence analysis', 'author': 'Jörnvall H.', 'year': '1987'} / Methods in protein sequence analysis by Jörnvall H. (1987)
{'key': 'e_1_2_2_42_2', 'first-page': '479', 'volume-title': 'Pulmonary surfactant', 'author': 'Fujiwara T.', 'year': '1984'} / Pulmonary surfactant by Fujiwara T. (1984)
{'key': 'e_1_2_2_43_2', 'first-page': '40', 'volume': '14', 'author': 'Suzuki Y.', 'year': '1983', 'journal-title': 'J. Jpn Med. Soc. Biol. Interface'} / J. Jpn Med. Soc. Biol. Interface by Suzuki Y. (1983)
{'key': 'e_1_2_2_44_2', 'first-page': '211', 'volume-title': 'Selected topics in perinatal medicine', 'author': 'Suzuki Y.', 'year': '1986'} / Selected topics in perinatal medicine by Suzuki Y. (1986)

Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 8:38 p.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 11:38 p.m.)
Indexed	13 hours, 24 minutes ago (Sept. 4, 2025, 10:27 a.m.)
Issued	37 years, 11 months ago (Oct. 1, 1987)
Published	37 years, 11 months ago (Oct. 1, 1987)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	37 years, 11 months ago (Oct. 1, 1987)

Funders 0

None

BibTeX

@article{CURSTEDT_1987, title={Two hydrophobic low‐molecular‐mass protein fractions of pulmonary surfactant: Characterization and biophysical activity}, volume={168}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1987.tb13414.x}, DOI={10.1111/j.1432-1033.1987.tb13414.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={CURSTEDT, Tore and JÖRNVALL, Hans and ROBERTSON, Bengt and BERGMAN, Tomas and BERGGREN, Per}, year={1987}, month=oct, pages={255–262} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        4
      ]
    ],
    "date-time": "2025-09-04T14:27:14Z",
    "timestamp": 1756996034173
  },
  "reference-count": 43,
  "publisher": "Wiley",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            3,
            3
          ]
        ],
        "date-time": "2005-03-03T00:00:00Z",
        "timestamp": 1109808000000
      },
      "content-version": "vor",
      "delay-in-days": 6363,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1987,
        10
      ]
    ]
  },
  "abstract": "<jats:p>Hydrophobic low\u2010molecular\u2010mass proteins were isolated from minced pig lungs and separated into two fractions. Electrophoresis of protein fraction 1 showed two major bands. Calculations of molecular masses from the electrophoretic mobilities are unreliable because of the extreme hydrophobicity of the peptides. However, the two bands were at positions corresponding to apparent molecular masses of about 3 kDa and 14 kDa, while sequence degradation disclosed only one major structure. Electrophoretic separation of protein fraction 2 revealed one band, at an apparent molecular mass of about 6 kDa. Microheterogeneities at the N terminus of both fractions were observed. However, the two fractions had different N\u2010terminal structures and amino acid compositions. Consequently they are concluded to represent different polypeptides without common segments. Bronchoalveolar lavage from humans also contains surfactant polypeptides and at least the fraction 2 peptide is highly similar in human and porcine surfactants.</jats:p><jats:p>Artificial surfactant preparations, obtained by recombination of protein fraction 1 or 2 with a mixture of synthetic phospholipids, were evaluated with the pulsating bubble method and in experiments on artificially ventilated premature newborn rabbits. The addition of protein fraction 1 to the phospholipid mixture improved surface adsorption from more than 300 s to about 2 s and reduced minimum surface tension from more than 20 mN/m to nearly 0 as measured with a pulsating bubble. When this surfactant preparation was instilled into the airways of newborn rabbits, the tidal volumes at insufflation pressure 25 cm H<jats:sub>2</jats:sub>O was increased about twentyfold compared to the volumes obtained in non\u2010treated controls. Preparations based on protein fraction 1 had better <jats:italic>in vitro</jats:italic> and <jats:italic>in vivo</jats:italic> properties than those based on protein fraction 2. Both these protein\u2010based preparations were decidedly more effective than phospholipids alone.</jats:p>",
  "DOI": "10.1111/j.1432-1033.1987.tb13414.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        3,
        4
      ]
    ],
    "date-time": "2005-03-04T01:38:10Z",
    "timestamp": 1109900290000
  },
  "page": "255-262",
  "source": "Crossref",
  "is-referenced-by-count": 209,
  "title": "Two hydrophobic low\u2010molecular\u2010mass protein fractions of pulmonary surfactant",
  "prefix": "10.1111",
  "volume": "168",
  "author": [
    {
      "given": "Tore",
      "family": "CURSTEDT",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Hans",
      "family": "J\u00d6RNVALL",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Bengt",
      "family": "ROBERTSON",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Tomas",
      "family": "BERGMAN",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Per",
      "family": "BERGGREN",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/ajplegacy.1973.224.4.788"
    },
    {
      "key": "e_1_2_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj1830731"
    },
    {
      "key": "e_1_2_2_4_2",
      "first-page": "562",
      "volume": "40",
      "author": "Katyal S. L.",
      "year": "1979",
      "journal-title": "Lab. Invest."
    },
    {
      "key": "e_1_2_2_5_2",
      "first-page": "408",
      "volume": "40",
      "author": "Claypool W. D.",
      "year": "1981",
      "journal-title": "Fed. Proc."
    },
    {
      "key": "e_1_2_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2760(81)90257-5"
    },
    {
      "key": "e_1_2_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(86)90026-4"
    },
    {
      "key": "e_1_2_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1203/00006450-198605000-00016"
    },
    {
      "key": "e_1_2_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1203/00006450-198608000-00009"
    },
    {
      "key": "e_1_2_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj2360085"
    },
    {
      "key": "e_1_2_2_11_2",
      "doi-asserted-by": "crossref",
      "first-page": "83",
      "DOI": "10.1159/000410001",
      "volume": "18",
      "author": "Benson B. J.",
      "year": "1984",
      "journal-title": "Prog. Respir. Res."
    },
    {
      "key": "e_1_2_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2760(84)90048-1"
    },
    {
      "key": "e_1_2_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.82.19.6379"
    },
    {
      "key": "e_1_2_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/317361a0"
    },
    {
      "key": "e_1_2_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(79)90092-0"
    },
    {
      "key": "e_1_2_2_16_2",
      "first-page": "1258",
      "volume": "134",
      "author": "Revak S. D.",
      "year": "1986",
      "journal-title": "Am. Rev. Respir. Dis."
    },
    {
      "key": "e_1_2_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/jappl.1980.49.1.34"
    },
    {
      "key": "e_1_2_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.3109/01902148509069678"
    },
    {
      "key": "e_1_2_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-2275(20)38173-6"
    },
    {
      "key": "e_1_2_2_20_2",
      "first-page": "336",
      "volume": "69",
      "author": "Suzuki Y.",
      "year": "1986",
      "journal-title": "Eur. J. Respir. Dis."
    },
    {
      "key": "e_1_2_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1172/JCI111483"
    },
    {
      "key": "e_1_2_2_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-2275(20)35198-1"
    },
    {
      "key": "e_1_2_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2760(74)90251-3"
    },
    {
      "key": "e_1_2_2_24_2",
      "unstructured": "Noack G. Berggren P. Curstedt T. Grossmann G. Herin P. Mortensson W. Nilsson R.&Robertson B.(1987)Acta Pediatr. Scand. in the press."
    },
    {
      "key": "e_1_2_2_25_2",
      "first-page": "116",
      "volume-title": "Hyaline membrane disease \u2013 surfactant and its pharmacological influence",
      "author": "Robertson B.",
      "year": "1987"
    },
    {
      "key": "e_1_2_2_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0378-4347(00)80353-9"
    },
    {
      "key": "e_1_2_2_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(71)90436-2"
    },
    {
      "key": "e_1_2_2_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)70226-3"
    },
    {
      "key": "e_1_2_2_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-2275(20)40190-7"
    },
    {
      "key": "e_1_2_2_30_2",
      "volume-title": "Methods in protein sequence analysis",
      "author": "Bergman T.",
      "year": "1987"
    },
    {
      "key": "e_1_2_2_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(77)90276-7"
    },
    {
      "key": "e_1_2_2_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/jappl.1977.43.2.198"
    },
    {
      "key": "e_1_2_2_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-6576.1982.tb01733.x"
    },
    {
      "key": "e_1_2_2_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1203/00006450-197804000-00001"
    },
    {
      "key": "e_1_2_2_35_2",
      "unstructured": "Robertson B.&Lachmann B.(1987)Exp. Lung Res. in the press."
    },
    {
      "key": "e_1_2_2_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1136/adc.55.10.758"
    },
    {
      "key": "e_1_2_2_37_2",
      "first-page": "125",
      "volume": "15",
      "author": "Kobayashi T.",
      "year": "1984",
      "journal-title": "J. Jpn Med. Soc. Biol. Interface"
    },
    {
      "key": "e_1_2_2_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00451913"
    },
    {
      "key": "e_1_2_2_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-3476(85)80416-9"
    },
    {
      "key": "e_1_2_2_40_2",
      "doi-asserted-by": "crossref",
      "first-page": "875",
      "DOI": "10.1152/jappl.1983.55.3.875",
      "volume": "55",
      "author": "Egan E. A.",
      "year": "1983",
      "journal-title": "J. Appl. Physiol."
    },
    {
      "key": "e_1_2_2_41_2",
      "volume-title": "Methods in protein sequence analysis",
      "author": "J\u00f6rnvall H.",
      "year": "1987"
    },
    {
      "key": "e_1_2_2_42_2",
      "first-page": "479",
      "volume-title": "Pulmonary surfactant",
      "author": "Fujiwara T.",
      "year": "1984"
    },
    {
      "key": "e_1_2_2_43_2",
      "first-page": "40",
      "volume": "14",
      "author": "Suzuki Y.",
      "year": "1983",
      "journal-title": "J. Jpn Med. Soc. Biol. Interface"
    },
    {
      "key": "e_1_2_2_44_2",
      "first-page": "211",
      "volume-title": "Selected topics in perinatal medicine",
      "author": "Suzuki Y.",
      "year": "1986"
    }
  ],
  "container-title": "European Journal of Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1987.tb13414.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1987.tb13414.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T04:38:41Z",
    "timestamp": 1700714321000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1987.tb13414.x"
    }
  },
  "subtitle": [
    "Characterization and biophysical activity"
  ],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1987,
        10
      ]
    ]
  },
  "references-count": 43,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1987,
          10
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1432-1033.1987.tb13414.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1987.tb13414.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1987,
        10
      ]
    ]
  }
}