DOI: 10.1111/j.1432-1033.1987.tb13368.x. Conformational properties of azurin in solution as determined from resolution‐enhanced Fourier‐transform infrared spectra

Conformational properties of azurin in solution as determined from resolution‐enhanced Fourier‐transform infrared spectra

10.1111/j.1432-1033.1987.tb13368.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Infrared spectra of the blue copper protein azurin and of apoazurin from P. fluorescens were obtained in aqueous solution. Using resolution enhancement procedures, a number of component bands were identified in the region of the amide I mode, and these bands were assigned to various components of protein secondary structure. A quantitative analysis of these infrared spectra indicates that the secondary structure of P. fluorescens azurin in solution is very similar to those determined previously by X‐ray diffraction for the crystals of azurins from other bacterial species. The major components of this structure are β strands and turns. Infrared spectra also evince a remarkable thermal stability of the native azurin. A significant unfolding of the protein could only be detected at temperatures above approximately 76°C. While the secondary structure of apoazurin is practically indistinguishable from that of the native protein at room temperature, the thermal stability of the apo form is significantly reduced.

Bibliography

SUREWICZ, W. K., SZABO, Art. G., & MANTSCH, H. H. (1987). Conformational properties of azurin in solution as determined from resolutionâenhanced Fourierâtransform infrared spectra. European Journal of Biochemistry, 167(3), 519â523. Portico.

Authors 3

Witold K. SUREWICZ (first)
Art. G. SZABO (additional)
Henry H. MANTSCH (additional)

References 33 Referenced 57

10.1007/BFb0116549
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{'key': 'e_1_2_1_18_2', 'first-page': '1', 'volume-title': 'Spectroscopy of biological systems', 'author': 'Mantsch H. H.', 'year': '1986'} / Spectroscopy of biological systems by Mantsch H. H. (1986)
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Moffatt D. G. Kauppinen J. K. Cameron D. G. Mantsch H. H.&Jones R. N.(1986)Computer programs for infrared spectrophotometry NRC Bulletin no. 18 Ottawa Canada.

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 8:32 p.m.)
Deposited	1 year, 10 months ago (Oct. 7, 2023, 7:11 a.m.)
Indexed	1 year ago (Aug. 22, 2024, 5:20 a.m.)
Issued	37 years, 11 months ago (Sept. 1, 1987)
Published	37 years, 11 months ago (Sept. 1, 1987)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	37 years, 11 months ago (Sept. 1, 1987)

Funders 0

None

BibTeX

@article{SUREWICZ_1987, title={Conformational properties of azurin in solution as determined from resolution‐enhanced Fourier‐transform infrared spectra}, volume={167}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1987.tb13368.x}, DOI={10.1111/j.1432-1033.1987.tb13368.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={SUREWICZ, Witold K. and SZABO, Art. G. and MANTSCH, Henry H.}, year={1987}, month=sep, pages={519–523} }

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