DOI: 10.1111/j.1432-1033.1987.tb11169.x. Nitrogen catabolite regulation of proline permease in <i>Saccharomyces cerevisiae</i>

Nitrogen catabolite regulation of proline permease in Saccharomyces cerevisiae

10.1111/j.1432-1033.1987.tb11169.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The proline permease gene PUT4 has been cloned. Nitrogen‐source regulation (‘ammonia sensitivity’) of this and at least two other amino‐acid permeases is believed to occur at two distinct levels, i.e. permease synthesis and permease activity. Therefore, PUT4 transcription/messenger stability was examined in the ammonia‐and proline‐grown wild type as well as in mutant strains supposedly affected at only one or at both of these levels. We report transcript‐level repression of proline permease synthesis in ammonia‐grown cells. Repression is lifted at this level in gdhCR, glnlts and gdhA mutants which exhibit pleiotropically derepressed permease and catabolic enzyme activities.On the other hand, the npil and npi2 mutations, formerly called mut2 and mut4, relieve an inactivation process which seems only to affect permeases. These mutations do not affect the detected PUT4 RNA level. The only known positive factor in proline permease regulation, the nitrogen permease reactivator protein Npr1, is believed to counteract the inactivation process on derepressing media. This protein appears to have an additional, indirect effect on PUT4 transcription/messenger stability: it would actually mediate repression via its activating effect on ammonia uptake.

Bibliography

JAUNIAUX, J., VANDENBOL, M., VISSERS, S., BROMAN, K., & GRENSON, M. (1987). Nitrogen catabolite regulation of proline permease in Saccharomyces cerevisiae. European Journal of Biochemistry, 164(3), 601â606. Portico.

Authors 5

Jean‐Claude JAUNIAUX (first)
Micheline VANDENBOL (additional)
Stephan VISSERS (additional)
Kathleen BROMAN (additional)
Marcelle GRENSON (additional)

References 35 Referenced 103

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10.1016/0005-2736(73)90121-1 / Biochim. Biophys. Acta by Magaña‐Schwenke N. (1973)
Grenson M. Muyldermans F. Broman K.&Vissers S.(1987)Life. Sci. Adv. Ser. C in the press.
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Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 8:22 p.m.)
Deposited	1 year, 11 months ago (Oct. 1, 2023, 2:30 p.m.)
Indexed	4 weeks, 2 days ago (Aug. 5, 2025, 9:07 a.m.)
Issued	38 years, 4 months ago (May 1, 1987)
Published	38 years, 4 months ago (May 1, 1987)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	38 years, 4 months ago (May 1, 1987)

Funders 0

None

BibTeX

@article{JAUNIAUX_1987, title={Nitrogen catabolite regulation of proline permease in Saccharomyces cerevisiae: Cloning of the PUT4 gene and study of PUT4 RNA levels in wild‐type and mutant strains}, volume={164}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1987.tb11169.x}, DOI={10.1111/j.1432-1033.1987.tb11169.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={JAUNIAUX, Jean‐Claude and VANDENBOL, Micheline and VISSERS, Stephan and BROMAN, Kathleen and GRENSON, Marcelle}, year={1987}, month=may, pages={601–606} }

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