DOI: 10.1111/j.1432-1033.1987.tb10736.x. Distinct patterns of glycosylation of colligin, a collagen‐binding glycoprotein, and SPARC (osteonectin), a secreted Ca<sup>2+</sup>‐binding glycoprotein

Distinct patterns of glycosylation of colligin, a collagen‐binding glycoprotein, and SPARC (osteonectin), a secreted Ca2+‐binding glycoprotein

10.1111/j.1432-1033.1987.tb10736.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Mouse parietal endoderm PYS cells were labelled with [2‐3H]mannose for 16–24 h. Colligin, an Mr‐47 000 collagen‐binding protein, and SPARC, a Mr‐43 000 protein, highly homologous to the Ca2+‐binding protein osteonectin, were isolated from labelled cell extracts and culture medium respectively. Glycopeptides obtained by exhaustive digestion with pronase were analysed by lectin‐affinity, ion‐exchange, and gel‐filtration chromatography and by paper chromatography of high‐mannose oligosaccharides after endo H release. The results show that the N‐linked carbohydrate chains of colligin are exclusively the high‐mannose type, of which (Man)8(GlcNAc)2 and (Man)9(GlcNAc)2 make up 77%. This carbohydrate structure provides strong evidence that colligin is a component of the endoplasmic reticulum, and argues against a role in cell‐surface interactions. By contrast to colligin, SPARC secreted by PYS cells contains predominantly a diantennary complex type of chain containing a variable number of sialic acid and core‐substituted fucose residues. Similar glycosylation patterns to those discussed above were seen in colligin isolated from primary mouse embryonic parietal endoderm cells and the murine 3T3 cell line, and in SPARC secreted by bovine corneal endothelial cells. Unlike the type‐IV‐collagen‐binding glycoprotein studied by Dennis, J., Waller, C. and Schirrmacher, V. [J. Cell Biol. 99, 1416–1423 (1984)], removal of N‐linked oligosaccharides from colligin had no effect on its binding to native type IV collagen.

Bibliography

HUGHES, R. C., TAYLOR, A., SAGE, H., & HOGAN, B. L. M. (1987). Distinct patterns of glycosylation of colligin, a collagenâbinding glycoprotein, and SPARC (osteonectin), a secreted Ca2+âbinding glycoprotein. European Journal of Biochemistry, 163(1), 57â65. Portico.

Authors 4

R. Colin HUGHES (first)
Amanda TAYLOR (additional)
Helene SAGE (additional)
Brigid L. M. HOGAN (additional)

References 41 Referenced 51

10.1016/S0021-9258(18)91102-6 / J. Biol. Chem. by Kurkinen M. (1984)
10.1016/S0014-4827(85)80036-7
10.1016/S0021-9258(17)38424-7
10.1083/jcb.103.1.223
10.1002/j.1460-2075.1986.tb04383.x
{'key': 'e_1_2_3_7_2', 'volume': '5', 'author': 'Mason I. J.', 'year': '1986', 'journal-title': 'EMBO J.'} / EMBO J. by Mason I. J. (1986)
10.1002/jcp.1041270305
10.1016/S0021-9258(17)43194-2
10.1093/nar/14.11.4483
10.1016/0092-8674(81)90037-4
10.1016/S0021-9258(19)68633-3 / J. Biol. Chem. by Termine J. D. (1981)
10.1016/S0021-9258(18)89422-4
10.1073/pnas.79.15.4540
10.1021/bi00267a025
10.1002/jcp.1040840103
10.1042/bj2110575
10.1016/0008-6215(83)88018-5
10.1021/bi00323a037
10.1042/bj2260487 / Biochem. J. by Hughes R. C. (1985)
10.1016/S0021-9258(19)69037-X / J. Biol. Chem. by Kornfeld K. (1981)
10.1515/bchm2.1968.349.2.961
10.1016/0014-5793(78)81116-8 / FEBS Lett. by Legler D. (1978)
10.1016/S0021-9258(18)34130-9 / J. Biol. Chem. by Townsend R. R. (1982)
10.1093/oxfordjournals.jbchem.a133925
{'key': 'e_1_2_3_26_2', 'first-page': '367', 'volume': '89', 'author': 'Martinez J.', 'year': '1977', 'journal-title': 'J. Lab. Clin. Med.'} / J. Lab. Clin. Med. by Martinez J. (1977)
10.1016/0006-291X(78)90318-2
10.1042/bj1840399
10.1111/j.1432-1033.1976.tb10246.x / Eur. J. Biochem. by Beinfema J. J. (1976)
10.1139/o86-026
10.1016/S0021-9258(17)42963-2 / J. Biol. Chem. by Williams D. B. (1984)
10.1016/S0021-9258(17)38567-8
10.1083/jcb.101.4.1270
10.1083/jcb.99.3.1076 / J. Cell Biol. by Rosenfeid M. G. (1984)
10.1083/jcb.101.2.460
10.1016/S0021-9258(19)68757-0
10.1111/j.0014-2956.1985.00569.x
10.1016/S0021-9258(18)88868-8 / J. Biol. Chem. by Lewis M. J. (1985)
10.1073/pnas.80.23.7165 / Proc. Natl Acad. Sci. USA by Liscum L. (1985)
10.1038/300274a0
10.1083/jcb.99.4.1416
10.1016/S0021-9258(19)84471-X

Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 8:05 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:41 p.m.)
Indexed	1 year, 3 months ago (May 8, 2024, 8:25 a.m.)
Issued	38 years, 7 months ago (Feb. 1, 1987)
Published	38 years, 7 months ago (Feb. 1, 1987)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	38 years, 7 months ago (Feb. 1, 1987)

Funders 0

None

BibTeX

@article{HUGHES_1987, title={Distinct patterns of glycosylation of colligin, a collagen‐binding glycoprotein, and SPARC (osteonectin), a secreted Ca2+‐binding glycoprotein: Evidence for the localisation of colligin in the endoplasmic reticulum}, volume={163}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1987.tb10736.x}, DOI={10.1111/j.1432-1033.1987.tb10736.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={HUGHES, R. Colin and TAYLOR, Amanda and SAGE, Helene and HOGAN, Brigid L. M.}, year={1987}, month=feb, pages={57–65} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        5,
        8
      ]
    ],
    "date-time": "2024-05-08T12:25:23Z",
    "timestamp": 1715171123291
  },
  "reference-count": 41,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            3,
            3
          ]
        ],
        "date-time": "2005-03-03T00:00:00Z",
        "timestamp": 1109808000000
      },
      "content-version": "vor",
      "delay-in-days": 6605,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1987,
        2
      ]
    ]
  },
  "abstract": "<jats:p>Mouse parietal endoderm PYS cells were labelled with [2\u2010<jats:sup>3</jats:sup>H]mannose for 16\u201324 h. Colligin, an <jats:italic>M</jats:italic><jats:sub>r</jats:sub>\u201047 000 collagen\u2010binding protein, and SPARC, a <jats:italic>M</jats:italic><jats:sub>r</jats:sub>\u201043 000 protein, highly homologous to the Ca<jats:sup>2+</jats:sup>\u2010binding protein osteonectin, were isolated from labelled cell extracts and culture medium respectively. Glycopeptides obtained by exhaustive digestion with pronase were analysed by lectin\u2010affinity, ion\u2010exchange, and gel\u2010filtration chromatography and by paper chromatography of high\u2010mannose oligosaccharides after endo H release. The results show that the N\u2010linked carbohydrate chains of colligin are exclusively the high\u2010mannose type, of which (Man)<jats:sub>8</jats:sub>(GlcNAc)<jats:sub>2</jats:sub> and (Man)<jats:sub>9</jats:sub>(GlcNAc)<jats:sub>2</jats:sub> make up 77%. This carbohydrate structure provides strong evidence that colligin is a component of the endoplasmic reticulum, and argues against a role in cell\u2010surface interactions. By contrast to colligin, SPARC secreted by PYS cells contains predominantly a diantennary complex type of chain containing a variable number of sialic acid and core\u2010substituted fucose residues. Similar glycosylation patterns to those discussed above were seen in colligin isolated from primary mouse embryonic parietal endoderm cells and the murine 3T3 cell line, and in SPARC secreted by bovine corneal endothelial cells. Unlike the type\u2010IV\u2010collagen\u2010binding glycoprotein studied by Dennis, J., Waller, C. and Schirrmacher, V. [<jats:italic>J. Cell Biol. 99</jats:italic>, 1416\u20131423 (1984)], removal of N\u2010linked oligosaccharides from colligin had no effect on its binding to native type IV collagen.</jats:p>",
  "DOI": "10.1111/j.1432-1033.1987.tb10736.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        3,
        4
      ]
    ],
    "date-time": "2005-03-04T01:05:56Z",
    "timestamp": 1109898356000
  },
  "page": "57-65",
  "source": "Crossref",
  "is-referenced-by-count": 51,
  "title": "Distinct patterns of glycosylation of colligin, a collagen\u2010binding glycoprotein, and SPARC (osteonectin), a secreted Ca<sup>2+</sup>\u2010binding glycoprotein",
  "prefix": "10.1111",
  "volume": "163",
  "author": [
    {
      "given": "R. Colin",
      "family": "HUGHES",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Amanda",
      "family": "TAYLOR",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Helene",
      "family": "SAGE",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Brigid L. M.",
      "family": "HOGAN",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_3_2_2",
      "doi-asserted-by": "crossref",
      "first-page": "5915",
      "DOI": "10.1016/S0021-9258(18)91102-6",
      "volume": "259",
      "author": "Kurkinen M.",
      "year": "1984",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_3_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0014-4827(85)80036-7"
    },
    {
      "key": "e_1_2_3_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(17)38424-7"
    },
    {
      "key": "e_1_2_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.103.1.223"
    },
    {
      "key": "e_1_2_3_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1986.tb04383.x"
    },
    {
      "key": "e_1_2_3_7_2",
      "volume": "5",
      "author": "Mason I. J.",
      "year": "1986",
      "journal-title": "EMBO J."
    },
    {
      "key": "e_1_2_3_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jcp.1041270305"
    },
    {
      "key": "e_1_2_3_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(17)43194-2"
    },
    {
      "key": "e_1_2_3_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/14.11.4483"
    },
    {
      "key": "e_1_2_3_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(81)90037-4"
    },
    {
      "key": "e_1_2_3_12_2",
      "doi-asserted-by": "crossref",
      "first-page": "10403",
      "DOI": "10.1016/S0021-9258(19)68633-3",
      "volume": "256",
      "author": "Termine J. D.",
      "year": "1981",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_3_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)89422-4"
    },
    {
      "key": "e_1_2_3_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.79.15.4540"
    },
    {
      "key": "e_1_2_3_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00267a025"
    },
    {
      "key": "e_1_2_3_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jcp.1040840103"
    },
    {
      "key": "e_1_2_3_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj2110575"
    },
    {
      "key": "e_1_2_3_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0008-6215(83)88018-5"
    },
    {
      "key": "e_1_2_3_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00323a037"
    },
    {
      "key": "e_1_2_3_20_2",
      "doi-asserted-by": "crossref",
      "first-page": "487",
      "DOI": "10.1042/bj2260487",
      "volume": "226",
      "author": "Hughes R. C.",
      "year": "1985",
      "journal-title": "Biochem. J."
    },
    {
      "key": "e_1_2_3_21_2",
      "doi-asserted-by": "crossref",
      "first-page": "6633",
      "DOI": "10.1016/S0021-9258(19)69037-X",
      "volume": "257",
      "author": "Kornfeld K.",
      "year": "1981",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_3_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1515/bchm2.1968.349.2.961"
    },
    {
      "key": "e_1_2_3_23_2",
      "doi-asserted-by": "crossref",
      "first-page": "255",
      "DOI": "10.1016/0014-5793(78)81116-8",
      "volume": "93",
      "author": "Legler D.",
      "year": "1978",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "e_1_2_3_24_2",
      "doi-asserted-by": "crossref",
      "first-page": "9704",
      "DOI": "10.1016/S0021-9258(18)34130-9",
      "volume": "257",
      "author": "Townsend R. R.",
      "year": "1982",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_3_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/oxfordjournals.jbchem.a133925"
    },
    {
      "key": "e_1_2_3_26_2",
      "first-page": "367",
      "volume": "89",
      "author": "Martinez J.",
      "year": "1977",
      "journal-title": "J. Lab. Clin. Med."
    },
    {
      "key": "e_1_2_3_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(78)90318-2"
    },
    {
      "key": "e_1_2_3_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj1840399"
    },
    {
      "key": "e_1_2_3_29_2",
      "doi-asserted-by": "crossref",
      "first-page": "441",
      "DOI": "10.1111/j.1432-1033.1976.tb10246.x",
      "volume": "63",
      "author": "Beinfema J. J.",
      "year": "1976",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "e_1_2_3_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1139/o86-026"
    },
    {
      "key": "e_1_2_3_31_2",
      "doi-asserted-by": "crossref",
      "first-page": "5105",
      "DOI": "10.1016/S0021-9258(17)42963-2",
      "volume": "259",
      "author": "Williams D. B.",
      "year": "1984",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_3_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(17)38567-8"
    },
    {
      "key": "e_1_2_3_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.101.4.1270"
    },
    {
      "key": "e_1_2_3_34_2",
      "doi-asserted-by": "crossref",
      "first-page": "1076",
      "DOI": "10.1083/jcb.99.3.1076",
      "volume": "99",
      "author": "Rosenfeid M. G.",
      "year": "1984",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "e_1_2_3_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.101.2.460"
    },
    {
      "key": "e_1_2_3_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)68757-0"
    },
    {
      "key": "e_1_2_3_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.0014-2956.1985.00569.x"
    },
    {
      "key": "e_1_2_3_38_2",
      "doi-asserted-by": "crossref",
      "first-page": "6926",
      "DOI": "10.1016/S0021-9258(18)88868-8",
      "volume": "260",
      "author": "Lewis M. J.",
      "year": "1985",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_3_39_2",
      "doi-asserted-by": "crossref",
      "first-page": "7165",
      "DOI": "10.1073/pnas.80.23.7165",
      "volume": "80",
      "author": "Liscum L.",
      "year": "1985",
      "journal-title": "Proc. Natl Acad. Sci. USA"
    },
    {
      "key": "e_1_2_3_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/300274a0"
    },
    {
      "key": "e_1_2_3_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.99.4.1416"
    },
    {
      "key": "e_1_2_3_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)84471-X"
    }
  ],
  "container-title": "European Journal of Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1987.tb10736.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1987.tb10736.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T19:41:20Z",
    "timestamp": 1700768480000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1987.tb10736.x"
    }
  },
  "subtitle": [
    "Evidence for the localisation of colligin in the endoplasmic reticulum"
  ],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1987,
        2
      ]
    ]
  },
  "references-count": 41,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1987,
          2
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1432-1033.1987.tb10736.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1987.tb10736.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1987,
        2
      ]
    ]
  }
}