DOI: 10.1111/j.1432-1033.1987.tb10729.x. High‐resolution solid‐state <sup>13</sup>C‐NMR study of carbons C‐5 and C‐12 of the chromophore of bovine rhodopsin

High‐resolution solid‐state 13C‐NMR study of carbons C‐5 and C‐12 of the chromophore of bovine rhodopsin

10.1111/j.1432-1033.1987.tb10729.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Solid‐state 13C magic‐angle spinning NMR spectroscopy has been employed to study the conformation of the 11‐cis‐retinylidene Schiff base chromophore in bovine rhodopsin. Spectra were obtained from lyophilized samples of bovine rhodopsin selectively 13C‐labeled at position C‐5 or C‐12 of the retinyl moiety, and reconstituted in the fully saturated branched‐chain phospholipid diphytanoyl glycerophosphocholine. Comparison of the NMR parameters for carbon C‐5 presented in this paper with those published for retinyl Schiff base model compounds and bacteriorhodopsin by Harbison and coworkers [Harbison et al. (1985) Biochemistry 24, 6955–6962], indicate that in bovine rhodopsin the C‐6–C‐7 single bond has the unperturbed cis conformation. This is in contrast to the 6‐S‐trans conformation found in bacteriorhodopsin. The NMR parameters for bovine [12‐13C]rhodopsin present evidence for the presence of a negative charge interacting with the retinyl moiety near C‐12, in agreement with the model for the opsin shift presented by Honig and Nakanishi and coworkers [Kakitani et al. (1985) Photochem. Photobiol. 41, 471–479].

Bibliography

MOLLEVANGER, L. C. P. J., KENTGENS, A. P. M., PARDOEN, J. A., COURTIN, J. M. L., VEEMAN, W. S., LUGTENBURG, J., & de GRIP, W. J. (1987). Highâresolution solidâstate 13CâNMR study of carbons Câ5 and Câ12 of the chromophore of bovine rhodopsin. European Journal of Biochemistry, 163(1), 9â14. Portico.

Authors 7

Leo C. P. J. MOLLEVANGER (first)
Arno P. M. KENTGENS (additional)
Johannes A. PARDOEN (additional)
Jacques M. L. COURTIN (additional)
Wibren S. VEEMAN (additional)
Johan LUGTENBURG (additional)
Willem J. de GRIP (additional)

References 36 Referenced 56

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 8:05 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:42 p.m.)
Indexed	1 year ago (July 31, 2024, 7:31 p.m.)
Issued	38 years, 6 months ago (Feb. 1, 1987)
Published	38 years, 6 months ago (Feb. 1, 1987)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	38 years, 6 months ago (Feb. 1, 1987)

Funders 0

None

BibTeX

@article{MOLLEVANGER_1987, title={High‐resolution solid‐state 13C‐NMR study of carbons C‐5 and C‐12 of the chromophore of bovine rhodopsin: Evidence for a 6‐S‐cis conformation with negative‐charge perturbation near C‐12}, volume={163}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1987.tb10729.x}, DOI={10.1111/j.1432-1033.1987.tb10729.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={MOLLEVANGER, Leo C. P. J. and KENTGENS, Arno P. M. and PARDOEN, Johannes A. and COURTIN, Jacques M. L. and VEEMAN, Wibren S. and LUGTENBURG, Johan and de GRIP, Willem J.}, year={1987}, month=feb, pages={9–14} }

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