DOI: 10.1111/j.1432-1033.1986.tb10066.x. Purification and characterization of human interleukin‐1β expressed in recombinant <i>Escherichia coli</i>

Purification and characterization of human interleukin‐1β expressed in recombinant Escherichia coli

10.1111/j.1432-1033.1986.tb10066.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The high‐level expression of human interleukin‐1β in Escherichia coli is described. The protein contributes about 12% of the total cell protein and is associated with the soluble cytoplasmic fraction of the cell. A method for the purification of the protein is given which is based on anion‐ and cation‐exchange chromatographies. The isolated protein, shown to be homogeneous by several analytical methods, has been characterized by amino acid analysis, N‐ and C‐terminal sequence analysis and analytical centrifugation. The protein is biologically active as demonstrated by two different in vitro assays, namely, the mononuclear cell factor (IL‐1/MCF) assay and lymphocyte activating factor (IL‐1/LAF) assay. The specific activities determined with the IL‐1/MCF and IL‐1/LAF assays, are 2 × 107 and 4 × 107 units mg−1, respectively.

Bibliography

WINGFIELD, P., PAYTON, M., TAVERNIER, J., BARNES, M., SHAW, A., ROSE, K., SIMONA, M. G., DEMCZUK, S., WILLIAMSON, K., & DAYER, J. (1986). Purification and characterization of human interleukinâ1Î² expressed in recombinant Escherichia coli. European Journal of Biochemistry, 160(3), 491â497. Portico.

Authors 10

Paul WINGFIELD (first)
Mark PAYTON (additional)
Jean TAVERNIER (additional)
Marjory BARNES (additional)
Alan SHAW (additional)
Keith ROSE (additional)
Marco G. SIMONA (additional)
Stephen DEMCZUK (additional)
Karen WILLIAMSON (additional)
Jean‐Michel DAYER (additional)

References 33 Referenced 105

10.1093/clinids/6.1.51
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{'key': 'e_1_2_2_18_2', 'first-page': '26', 'volume-title': 'Laboratory techniques in biochemistry and molecular biology', 'author': 'Allen G.', 'year': '1981'} / Laboratory techniques in biochemistry and molecular biology by Allen G. (1981)
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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 7:49 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 8:02 a.m.)
Indexed	3 weeks, 3 days ago (Aug. 2, 2025, 12:30 a.m.)
Issued	38 years, 9 months ago (Nov. 1, 1986)
Published	38 years, 9 months ago (Nov. 1, 1986)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	38 years, 9 months ago (Nov. 1, 1986)

Funders 0

None

BibTeX

@article{WINGFIELD_1986, title={Purification and characterization of human interleukin‐1β expressed in recombinant Escherichia coli}, volume={160}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1986.tb10066.x}, DOI={10.1111/j.1432-1033.1986.tb10066.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={WINGFIELD, Paul and PAYTON, Mark and TAVERNIER, Jean and BARNES, Marjory and SHAW, Alan and ROSE, Keith and SIMONA, Marco G. and DEMCZUK, Stephen and WILLIAMSON, Karen and DAYER, Jean‐Michel}, year={1986}, month=nov, pages={491–497} }

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