DOI: 10.1111/j.1432-1033.1986.tb09970.x. Pyrophosphate‐caused inhibition of the aminoacylation of tRNA by the leucyl‐tRNA synthetase from <i>Neurospora crassa</i>

Pyrophosphate‐caused inhibition of the aminoacylation of tRNA by the leucyl‐tRNA synthetase from Neurospora crassa

10.1111/j.1432-1033.1986.tb09970.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Inorganic pyrophosphate inhibits the aminoacylation of tRNALeu by the leucyl‐tRNA synthetase from Neurospora crassa giving very low Ki, PPiapp. values of 3–20 μM. The inhibition by pyrophosphate, together with earlier kinetic data, suggest a reaction mechanism where leucine, ATP and tRNA are bound to the enzyme in almost random order, and pyrophosphate is dissociated before the rate‐limiting step. A kinetic analysis of this mechanism shows that the measured Kiapp. values do not give the real dissociation constant but it is about 0.4 mM. Other dissociation constants are 90 μM for leucine, 2.2 mM for ATP and 1 μM for tRNALeu. At the approximate conditions of the living cell (2 mM ATP, 100 μM leucine and 150 μM PPi) the leucyl‐tRNA synthetase is about 85% inhibited by pyrophosphate.

Bibliography

AIRAS, R. K., & CRAMER, F. (1986). Pyrophosphateâcaused inhibition of the aminoacylation of tRNA by the leucylâtRNA synthetase from Neurospora crassa. European Journal of Biochemistry, 160(2), 291â296. Portico.

Authors 2

R. Kalervo AIRAS (first)
Friedrich CRAMER (additional)

References 31 Referenced 13

10.1016/S0021-9258(18)97667-2
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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 7:46 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 12:57 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 11:40 a.m.)
Issued	38 years, 11 months ago (Oct. 1, 1986)
Published	38 years, 11 months ago (Oct. 1, 1986)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	38 years, 11 months ago (Oct. 1, 1986)

Funders 0

None

BibTeX

@article{AIRAS_1986, title={Pyrophosphate‐caused inhibition of the aminoacylation of tRNA by the leucyl‐tRNA synthetase from Neurospora crassa}, volume={160}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1986.tb09970.x}, DOI={10.1111/j.1432-1033.1986.tb09970.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={AIRAS, R. Kalervo and CRAMER, Friedrich}, year={1986}, month=oct, pages={291–296} }

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