DOI: 10.1111/j.1432-1033.1986.tb09926.x. The amino‐acid sequence of the 2S sulphur‐rich proteins from seeds of Brazil nut (<i>Bertholletia excelsa</i> H.B.K.)

The amino‐acid sequence of the 2S sulphur‐rich proteins from seeds of Brazil nut (Bertholletia excelsa H.B.K.)

10.1111/j.1432-1033.1986.tb09926.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Storage proteins of the albumin solubility fraction from seeds of Bertholletia excelsa H.B.K. were separated by reversed‐phase high‐performance liquid chromatography and their primary structures were determined by gas‐phase sequencing on intact polypeptides and on the overlapping tryptic and thermolysin peptides. The 2S storage proteins consist of two subunits linked by disulphide bridges. The large subunit (8.5 kDa) is expressed in at least six different isoforms while the small subunit (3.6 kDa) consists of only one form. These proteins are extremely rich in glutamine, glutamic acid, arginine and the sulphur‐containing amino acids cysteine and methionine. One of the variants even contains a sequence of six methionine residues in a row. Comparison with known sequences of 2S proteins of other dicotyledonous plants shows limited but distinct sequence homology. In particular, the positions of the cysteine residues relative to each other appear to be completely conserved, suggesting that tertiary structure constraints imposed by disulphide bridges dominate sequence conservation. It has been proposed that the two subunits of a related protein (the Brassica napus storage protein) is cleaved from a precursor polypeptide [Crouch, M. L., Tenbarge, K. M., Simon, A. E. & Ferl, R. (1983) J. Mol. Appl. Genet. 2, 273–283]. The amino acid sequence homology of the Brazil nut protein with the former suggests that a similar protein processing event could occur.

Bibliography

AMPE, C., VAN DAMME, J., de CASTRO, L. A. B., SAMPAIO, M. J. A. M., VAN MONTAGU, M., & VANDEKERCKHOVE, J. (1986). The aminoâacid sequence of the 2S sulphurârich proteins from seeds of Brazil nut (Bertholletia excelsa H.B.K.). European Journal of Biochemistry, 159(3), 597â601. Portico.

Authors 6

Christophe AMPE (first)
Jozef VAN DAMME (additional)
Luiz A. B. de CASTRO (additional)
Maria José A. M. SAMPAIO (additional)
Marc VAN MONTAGU (additional)
Joël VANDEKERCKHOVE (additional)

References 12 Referenced 101

{'key': 'e_1_2_3_2_2', 'first-page': '119', 'volume': '35', 'author': 'Higgins T. J. V.', 'year': '1984', 'journal-title': 'Annu. Rev. Plant. Physiol.'} / Annu. Rev. Plant. Physiol. by Higgins T. J. V. (1984)
{'key': 'e_1_2_3_3_2', 'first-page': '16', 'volume-title': 'The vegetable proteins', 'author': 'Osborne T. B.', 'year': '1924'} / The vegetable proteins by Osborne T. B. (1924)
10.1002/j.1537-2197.1981.tb06354.x
10.1085/jgp.29.3.149
10.1016/0076-6879(70)19075-6 / Methods Enzymol. by Kasell B. (1970)
10.1016/S0021-9258(18)33344-1
10.1042/bj2250239
{'key': 'e_1_2_3_9_2', 'first-page': '273', 'volume': '2', 'author': 'Crouch M. L.', 'year': '1983', 'journal-title': 'J. Mol. Appl. Genet.'} / J. Mol. Appl. Genet. by Crouch M. L. (1983)
10.1515/bchm2.1981.362.2.1131
10.1021/bi00898a036
10.1016/0005-2795(72)90119-5
10.1093/oxfordjournals.jbchem.a130295

Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 7:44 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 6:12 p.m.)
Indexed	1 month ago (Aug. 5, 2025, 8:23 a.m.)
Issued	39 years ago (Sept. 1, 1986)
Published	39 years ago (Sept. 1, 1986)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	39 years ago (Sept. 1, 1986)

Funders 0

None

BibTeX

@article{AMPE_1986, title={The amino‐acid sequence of the 2S sulphur‐rich proteins from seeds of Brazil nut (Bertholletia excelsa H.B.K.)}, volume={159}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1986.tb09926.x}, DOI={10.1111/j.1432-1033.1986.tb09926.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={AMPE, Christophe and VAN DAMME, Jozef and de CASTRO, Luiz A. B. and SAMPAIO, Maria José A. M. and VAN MONTAGU, Marc and VANDEKERCKHOVE, Joël}, year={1986}, month=sep, pages={597–601} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        5
      ]
    ],
    "date-time": "2025-08-05T12:23:45Z",
    "timestamp": 1754396625057
  },
  "reference-count": 12,
  "publisher": "Wiley",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            3,
            3
          ]
        ],
        "date-time": "2005-03-03T00:00:00Z",
        "timestamp": 1109808000000
      },
      "content-version": "vor",
      "delay-in-days": 6758,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1986,
        9
      ]
    ]
  },
  "abstract": "<jats:p>Storage proteins of the albumin solubility fraction from seeds of <jats:italic>Bertholletia excelsa</jats:italic> H.B.K. were separated by reversed\u2010phase high\u2010performance liquid chromatography and their primary structures were determined by gas\u2010phase sequencing on intact polypeptides and on the overlapping tryptic and thermolysin peptides. The 2S storage proteins consist of two subunits linked by disulphide bridges. The large subunit (8.5 kDa) is expressed in at least six different isoforms while the small subunit (3.6 kDa) consists of only one form. These proteins are extremely rich in glutamine, glutamic acid, arginine and the sulphur\u2010containing amino acids cysteine and methionine. One of the variants even contains a sequence of six methionine residues in a row. Comparison with known sequences of 2S proteins of other dicotyledonous plants shows limited but distinct sequence homology. In particular, the positions of the cysteine residues relative to each other appear to be completely conserved, suggesting that tertiary structure constraints imposed by disulphide bridges dominate sequence conservation. It has been proposed that the two subunits of a related protein (the <jats:italic>Brassica napus</jats:italic> storage protein) is cleaved from a precursor polypeptide [Crouch, M. L., Tenbarge, K. M., Simon, A. E. &amp; Ferl, R. (1983) <jats:italic>J. Mol. Appl. Genet. 2</jats:italic>, 273\u2013283]. The amino acid sequence homology of the Brazil nut protein with the former suggests that a similar protein processing event could occur.</jats:p>",
  "DOI": "10.1111/j.1432-1033.1986.tb09926.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        3,
        4
      ]
    ],
    "date-time": "2005-03-04T00:44:34Z",
    "timestamp": 1109897074000
  },
  "page": "597-601",
  "source": "Crossref",
  "is-referenced-by-count": 101,
  "title": "The amino\u2010acid sequence of the 2S sulphur\u2010rich proteins from seeds of Brazil nut (<i>Bertholletia excelsa</i> H.B.K.)",
  "prefix": "10.1111",
  "volume": "159",
  "author": [
    {
      "given": "Christophe",
      "family": "AMPE",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Jozef",
      "family": "VAN DAMME",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Luiz A. B.",
      "family": "de CASTRO",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Maria Jos\u00e9 A. M.",
      "family": "SAMPAIO",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Marc",
      "family": "VAN MONTAGU",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Jo\u00ebl",
      "family": "VANDEKERCKHOVE",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_3_2_2",
      "first-page": "119",
      "volume": "35",
      "author": "Higgins T. J. V.",
      "year": "1984",
      "journal-title": "Annu. Rev. Plant. Physiol."
    },
    {
      "key": "e_1_2_3_3_2",
      "first-page": "16",
      "volume-title": "The vegetable proteins",
      "author": "Osborne T. B.",
      "year": "1924"
    },
    {
      "key": "e_1_2_3_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1537-2197.1981.tb06354.x"
    },
    {
      "key": "e_1_2_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1085/jgp.29.3.149"
    },
    {
      "key": "e_1_2_3_6_2",
      "doi-asserted-by": "crossref",
      "first-page": "853",
      "DOI": "10.1016/0076-6879(70)19075-6",
      "volume": "19",
      "author": "Kasell B.",
      "year": "1970",
      "journal-title": "Methods Enzymol."
    },
    {
      "key": "e_1_2_3_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)33344-1"
    },
    {
      "key": "e_1_2_3_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj2250239"
    },
    {
      "key": "e_1_2_3_9_2",
      "first-page": "273",
      "volume": "2",
      "author": "Crouch M. L.",
      "year": "1983",
      "journal-title": "J. Mol. Appl. Genet."
    },
    {
      "key": "e_1_2_3_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1515/bchm2.1981.362.2.1131"
    },
    {
      "key": "e_1_2_3_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00898a036"
    },
    {
      "key": "e_1_2_3_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2795(72)90119-5"
    },
    {
      "key": "e_1_2_3_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/oxfordjournals.jbchem.a130295"
    }
  ],
  "container-title": "European Journal of Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1986.tb09926.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1986.tb09926.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T23:12:46Z",
    "timestamp": 1700781166000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1986.tb09926.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1986,
        9
      ]
    ]
  },
  "references-count": 12,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1986,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1432-1033.1986.tb09926.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1986.tb09926.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1986,
        9
      ]
    ]
  }
}