DOI: 10.1111/j.1432-1033.1985.tb09349.x. Proteinase inhibitors and dendrotoxins

10.1111/j.1432-1033.1985.tb09349.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The amino acid sequences of four presynaptically active toxins from mamba snake venom (termed ‘dendrotoxins’) were compared systematically with homologous sequences of members of the proteinase inhibitor family (Kunitz). A comparison based on the complete sequences revealed that relatively few amino acid changes are necessary to abolish antiprotease activity and convert a proteinase inhibitor into a dendrotoxin. When comparison centred only on the sequence segments known to comprise the antiprotease site of bovine pancreatic trypsin inhibitor, the dendrotoxins were clearly classified apart from all the known inhibitors. Since the mode of action of the bovine pancreatic trypsin/kallikrein inhibitor involves β sheet formation with the enzyme, predictions were obtained for this secondary structure in the region of the ‘antiprotease site’ throughout the homologues. Again, the dendrotoxins were clearly distinguished from the inhibitors.Structure/activity analyses, based on the crystal structures of inhibitor/enzyme complexes, suggest that unlike proteinase inhibitors, dendrotoxins might specifically co‐ordinate the active‐site ‘catalytic’ histidine residues of serine proteases. Although the significance of this remains to be studied, the presynaptic target is expected to involve an as yet uncharacterised member of the serine protease family.

Bibliography

DUFTON, M. J. (1985). Proteinase inhibitors and dendrotoxins. European Journal of Biochemistry, 153(3), 647â654. Portico.

Authors 1

Mark J. DUFTON (first)

References 26 Referenced 79

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{'key': 'e_1_2_3_26_2', 'first-page': '222', 'volume': '79', 'author': 'Harvey A. L.', 'year': '1984', 'journal-title': 'J. Physiol. (Paris)'} / J. Physiol. (Paris) by Harvey A. L. (1984)
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Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 7:14 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 8:19 a.m.)
Indexed	1 month ago (Aug. 2, 2025, 1:23 a.m.)
Issued	39 years, 9 months ago (Dec. 1, 1985)
Published	39 years, 9 months ago (Dec. 1, 1985)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	39 years, 9 months ago (Dec. 1, 1985)

Funders 0

None

BibTeX

@article{DUFTON_1985, title={Proteinase inhibitors and dendrotoxins: Sequence classification, structural prediction and structure/activity}, volume={153}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1985.tb09349.x}, DOI={10.1111/j.1432-1033.1985.tb09349.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={DUFTON, Mark J.}, year={1985}, month=dec, pages={647–654} }

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