DOI: 10.1111/j.1432-1033.1985.tb09281.x. Cross‐linking of bovine mitochondrial H<sup>+</sup>‐ATPase by copper

Cross‐linking of bovine mitochondrial H+‐ATPase by copper–o‐phenanthroline

10.1111/j.1432-1033.1985.tb09281.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The nearest neighbor relationships between the F0 subunits of bovine mitochondrial H+‐ATPase were studied by using copper–o‐phenanthroline, an SH‐oxidizing cross‐linking reagent. The cross‐linked samples of purified H+‐ATPase, F1‐ATPase or F0 were analyzed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS‐PAGE) and the disulfide cross‐linked polypeptides were identified by enzyme‐linked immunosorbent assay and immunoblot transfer using subunit specific antisera. SDS‐PAGE of H+‐ATPase showed several cross‐links, although none involved subunits of F0 sector linked to those of F1. Both H+‐ATPase and F0 showed formation of a 45‐kDa product. Upon reduction, the 45‐kDa component gave rise to a 21‐kDa band, identified as oligomycin‐sensitivity‐conferring protein (OSCP), and a 24‐kDa band. These two proteins thus appear to be near neighbors with their cysteine residues in close proximity with each other. Under the conditions of cross‐linking, there was a concentration‐dependent decrease in the Pi‐ATP exchange activity of the intact H+ ‐ATPase as well as of H+‐ATPase reconstituted with copper–o‐phenanthroline‐treated F0 and untreated F1. The site of inhibition appeared to residue in the F0 sector. Loss of Pi‐ATP exchange occurred at the same time as formation of the 45‐kDa product. Our present data showing copper–o‐phenanthroline‐induced interactions of the 24‐kDa protein with the OSCP and simultaneous inactivation of Pi‐ATP exchange activity of the complex strengthen earlier suggestions [Hadikusumo, R. G., Hertzog, P. J. & Marzuki, S. (1984) Biochim. Biophys. Acta 765, 258–267] that the 24‐kDa protein may be a bona fide subunit of F0.

Bibliography

TOROK, K., & JOSHI, S. (1985). Crossâlinking of bovine mitochondrial H+âATPase by copperâoâphenanthroline. European Journal of Biochemistry, 153(1), 155â159. Portico.

Authors 2

Katalin TOROK (first)
Saroj JOSHI (additional)

References 34 Referenced 19

10.1016/S0021-9258(19)40903-4
10.1016/S0021-9258(19)86880-1
10.1111/j.1432-1033.1979.tb02046.x
10.1016/S0021-9258(17)30143-6
10.1146/annurev.bi.49.070180.000551
10.1042/bj1900273 / Biochem. J. by Apps D. K. (1983)
10.1016/S0021-9258(17)33609-8
10.1016/0005-2728(78)90110-X
10.1016/S0021-9258(19)86685-1
10.1016/S0021-9258(19)86645-0
10.1016/S0021-9258(19)86325-1
{'key': 'e_1_2_3_13_2', 'first-page': '233', 'volume-title': 'Membrane proteins in energy transduction', 'author': 'Senior A. E.', 'year': '1979'} / Membrane proteins in energy transduction by Senior A. E. (1979)
10.1021/bi00775a006
10.1016/S0021-9258(19)68140-8
10.1007/BF00271191
10.1007/BF01648909
10.1016/0076-6879(79)55052-6 / Methods Enzymol. by Racker E. (1979)
{'key': 'e_1_2_3_19_2', 'first-page': '63', 'volume-title': 'Function and molecular aspects ofbiomembrane transport', 'author': 'Sebald W.', 'year': '1979'} / Function and molecular aspects ofbiomembrane transport by Sebald W. (1979)
10.1016/S0021-9258(19)69087-3 / J. Biol. Chem. by Todd R. D. (1981)
10.1111/j.1432-1033.1983.tb07346.x
10.1016/S0021-9258(18)90808-2
10.1016/S0021-9258(19)44736-4
10.1021/bi00844a049
10.1073/pnas.76.9.4350 / Proc. Natl Acad. Sci. USA by Towbin H. (1979)
10.1016/S0021-9258(18)93583-0
10.1038/227680a0
10.1016/S0021-9258(18)94333-4
10.1016/0005-2728(81)90057-8
10.1016/S0021-9258(17)43095-X
10.1016/0005-2728(84)90164-6
10.1016/0005-2728(79)90186-5 / Biochim. Biophys. Acta by Jong L. (1979)
10.1016/0003-2697(84)90121-0
10.1016/S0021-9258(18)43413-8
10.1016/S0021-9258(18)91008-2

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 7:13 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 7:38 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 7:05 p.m.)
Issued	39 years, 9 months ago (Nov. 1, 1985)
Published	39 years, 9 months ago (Nov. 1, 1985)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	39 years, 9 months ago (Nov. 1, 1985)

Funders 0

None

BibTeX

@article{TOROK_1985, title={Cross‐linking of bovine mitochondrial H+‐ATPase by copper–o‐phenanthroline: Interaction of the oligomycin‐sensitivity‐conferring protein with a 24‐kDa protein}, volume={153}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1985.tb09281.x}, DOI={10.1111/j.1432-1033.1985.tb09281.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={TOROK, Katalin and JOSHI, Saroj}, year={1985}, month=nov, pages={155–159} }

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