DOI: 10.1111/j.1432-1033.1985.tb09082.x. Amino acid sequence of histone H1 at the ADP‐ribose‐accepting site and ADP‐ribose · histone‐H1 adduct as an inhibitor of cyclic‐AMP‐dependent phosphorylation

Amino acid sequence of histone H1 at the ADP‐ribose‐accepting site and ADP‐ribose · histone‐H1 adduct as an inhibitor of cyclic‐AMP‐dependent phosphorylation

10.1111/j.1432-1033.1985.tb09082.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The ADP‐ribosylation site of histone H1 from calf thymus by purified hen liver nuclear ADP‐ribosyltransferase was determined and effects of the ADP‐ribose · histone‐H1 adduct on cAMP‐dependent phosphorylation of the histone H1 were investigated.ADP‐ribosylated histone H1 was prepared by incubation of histone H1, 1 mM [adenylate‐32P]NAD and the purified ADP‐ribosyltransferase. N‐Bromosuccinimide‐directed bisection of ADP‐ribosylated histone H1 showed that the NH2‐terminal fragment (Mr= 6000) was modified and contained serine residue 38, the site of phosphorylation by cAMP‐dependent protein kinase. Digestion of the NH2‐terminal fragment with cathepsin D and trypsin, and purification of this fragment, using high‐performance liquid chromatography, yielded a radiolabelled single peptide corresponding to residues 29–34 of histone H1, containing the arginine residue as the ADP‐ribosylation site. These results indicate that ADP‐ribosylation of histone H1 occurs at the arginine residue 34, sequenced at the NH2‐terminal side of the phosphate‐accepting serine residue 38.Phosphorylation of histone H1 from calf thymus by cAMP‐dependent protein kinase was markedly reduced when histone H1 was ADP‐ribosylated. Kinetic studies of phosphorylation revealed that ADP‐ribosylated histone H1 was a linear competitive inhibitor of histone H1 and a linear non‐competitive inhibitor of ATP.

Bibliography

USHIROYAMA, T., TANIGAWA, Y., TSUCHIYA, M., MATSUURA, R., UEKI, M., SUGIMOTO, O., & SHIMOYAMA, M. (1985). Amino acid sequence of histone H1 at the ADPâriboseâaccepting site and ADPâribose Â· histoneâH1 adduct as an inhibitor of cyclicâAMPâdependent phosphorylation. European Journal of Biochemistry, 151(1), 173â177. Portico.

Authors 7

Takahisa USHIROYAMA (first)
Yoshinori TANIGAWA (additional)
Mikako TSUCHIYA (additional)
Ryoji MATSUURA (additional)
Minoru UEKI (additional)
Osamu SUGIMOTO (additional)
Makoto SHIMOYAMA (additional)

References 29 Referenced 23

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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 7 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:35 a.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 3:08 p.m.)
Issued	40 years ago (Aug. 1, 1985)
Published	40 years ago (Aug. 1, 1985)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	40 years ago (Aug. 1, 1985)

Funders 0

None

BibTeX

@article{USHIROYAMA_1985, title={Amino acid sequence of histone H1 at the ADP‐ribose‐accepting site and ADP‐ribose · histone‐H1 adduct as an inhibitor of cyclic‐AMP‐dependent phosphorylation}, volume={151}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1985.tb09082.x}, DOI={10.1111/j.1432-1033.1985.tb09082.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={USHIROYAMA, Takahisa and TANIGAWA, Yoshinori and TSUCHIYA, Mikako and MATSUURA, Ryoji and UEKI, Minoru and SUGIMOTO, Osamu and SHIMOYAMA, Makoto}, year={1985}, month=aug, pages={173–177} }

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