DOI: 10.1111/j.1432-1033.1985.tb09061.x. Phosphorylation of the ribosomal protein S6 during agonist‐induced exocytosis in exocrine glands is catalyzed by calcium‐phospholipid‐dependent protein kinase (protein kinase C)

Phosphorylation of the ribosomal protein S6 during agonist‐induced exocytosis in exocrine glands is catalyzed by calcium‐phospholipid‐dependent protein kinase (protein kinase C)

10.1111/j.1432-1033.1985.tb09061.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The ribosomal protein S6 in exocrine cells is phosphorylated during stimulated during stimulation of exocytosis by cAMP‐dependent or calcium‐dependent agonists. Under both conditions the same tryptic S6 phosphopeptides (termed A, B, and C) were found [Padel, Kruppa, Jahn & Söling (1983) FEBS Lett. 159, 112–118]. Studies have now been made of the phosphorylation pattern of protein S6 from purified guinea pig parotid ribosomes following in vitro phosphorylation with calmodulin‐dependent, phospholipid‐dependent, and cAMP‐dependent protein kinases. Only the phospholipid‐dependent enzyme led to the phosphorylation of peptides A, B, and C, while the cAMP‐dependent enzyme phosphorylated only peptides A and C, and the calmodulin‐dependent enzyme did not phosphorylate any of the phosphopeptides found in S6 from unstimulated or stimulated intact cells. Guinea pig parotid microsomes contain substantial phospholipid‐dependent protein kinase activity. Stimulation of intact parotid glands with tetradecanolyphorbol acetate led to a significant phosphorylation of S6 and a similar tryptic S6 phosphopeptide pattern as seen with carbamoylcholine. It is concluded that activation of phospholipid‐dependent protein kinase is responsible for the phosphorylation of protein S6 during stimulation with calcium‐dependent and cAMP‐dependent secretagogues.

Bibliography

PADEL, U., & SÃLING, H. (1985). Phosphorylation of the ribosomal protein S6 during agonistâinduced exocytosis in exocrine glands is catalyzed by calciumâphospholipidâdependent protein kinase (protein kinase C). European Journal of Biochemistry, 151(1), 1â10. Portico.

Authors 2

Ulrike PADEL (first)
Hans‐Dieter SÖLING (additional)

References 56 Referenced 27

10.1073/pnas.78.11.6903
10.1016/S0006-291X(81)80042-3
10.1016/S0021-9258(19)68824-1
{'key': 'e_1_2_2_5_2', 'first-page': '39', 'volume': '4', 'author': 'Kanamori T.', 'year': '1982', 'journal-title': 'Biochem. Int.'} / Biochem. Int. by Kanamori T. (1982)
10.1016/S0021-9258(18)34049-3
10.1083/jcb.95.3.903
10.1083/jcb.95.3.909
10.1016/0014-5793(83)80121-5
10.1016/0014-5793(83)80427-X
10.1111/j.1432-1033.1982.tb19785.x
10.1016/0014-5793(82)80910-1
10.1083/jcb.97.4.1294
10.1073/pnas.80.22.6858
10.1016/S0021-9258(17)44015-4
10.1016/S0021-9258(17)43679-9
10.1016/S0021-9258(17)44200-1
10.1016/S0021-9258(18)91112-9 / J. Biol. Chem. by Novak‐Hofer J. (1984)
{'key': 'e_1_2_2_19_2', 'first-page': '201', 'volume': '20', 'author': 'Schmidt H. W.', 'year': '1979', 'journal-title': 'Eur. J. Cell Biol.'} / Eur. J. Cell Biol. by Schmidt H. W. (1979)
10.1042/bj2080205
10.1016/0076-6879(55)01021-5
10.1016/S0021-9258(17)32803-X / J. Biol. Chem. by Schubart U. K. (1977)
10.1038/227680a0
10.1016/0014-5793(82)81231-3
10.1016/0006-291X(82)90712-4
10.1021/bi00645a007
10.1016/S0021-9258(17)33471-3
10.1016/0003-2697(76)90527-3
10.1016/0014-5793(83)80846-1
10.1016/S0021-9258(18)34459-4
10.1016/0014-5793(82)81234-9
10.1016/0006-291X(83)90828-8
10.1111/j.1432-1033.1985.tb08879.x
10.1016/B978-0-12-452808-6.50008-6
10.1016/S0021-9258(17)43541-1
10.1016/S0021-9258(18)89469-8
10.1073/pnas.81.20.6408
10.1016/0065-2571(85)90044-5
Reference deleted.
10.1016/0005-2760(81)90192-2
10.1113/jphysiol.1957.sp005690
10.1042/bj1420583
10.1038/306067a0
10.1016/S0021-9258(17)43262-5
10.1038/309063a0
10.1038/309562a0
10.1111/j.1432-1033.1984.tb08431.x
10.1042/bj2200345
10.1042/bj2060555
10.1016/S0021-9258(18)32273-7
10.1016/0014-5793(83)80015-5
10.1016/0014-5793(84)81369-1
10.1016/S0021-9258(18)43230-9
10.1016/0014-5793(84)80484-6
10.1073/pnas.82.2.272
10.1073/pnas.82.3.742
10.1016/S0021-9258(17)44016-6 / J. Biol. Chem. by Burkhard S. J. (1983)

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 7 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:36 a.m.)
Indexed	1 year, 7 months ago (Jan. 13, 2024, 11:21 a.m.)
Issued	40 years ago (Aug. 1, 1985)
Published	40 years ago (Aug. 1, 1985)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	40 years ago (Aug. 1, 1985)

Funders 0

None

BibTeX

@article{PADEL_1985, title={Phosphorylation of the ribosomal protein S6 during agonist‐induced exocytosis in exocrine glands is catalyzed by calcium‐phospholipid‐dependent protein kinase (protein kinase C): Experiments with guinea pig parotid glands}, volume={151}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1985.tb09061.x}, DOI={10.1111/j.1432-1033.1985.tb09061.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={PADEL, Ulrike and SÖLING, Hans‐Dieter}, year={1985}, month=aug, pages={1–10} }

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