DOI: 10.1111/j.1432-1033.1985.tb08829.x. Cloning, nucleotide sequencing and expression of cDNAs encoding mouse urokinase‐type plasminogen activator

Cloning, nucleotide sequencing and expression of cDNAs encoding mouse urokinase‐type plasminogen activator

10.1111/j.1432-1033.1985.tb08829.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Controlled extracellular proteolysis is catalyzed in part by the secretion of plasminogen activators. As a step in the study of the expression of these enzymes in mouse tissues, we have isolated five cDNAs encoding the mouse urokinase‐type plasminogen activator from a cDNA library prepared with size‐selected mRNA from MSV‐transformed 3T3 cells. The longest cDNA insert contains the entire coding region of mouse urokinase, 58 base pairs of the 5′ non‐coding region, and the entire 3′ non‐coding region, which is 942 base pairs long. The deduced protein sequence, which starts with a signal peptide of 20 amino acids, shows extensive homology to that of human and porcine urokinase. However, in contrast to these enzymes, mouse urokinase contains no N‐glycosylation site. Bacteria harbouring one of the recombinant plasmids synthesize and secrete into their periplasm a protease indistinguishable from mouse urokinase.

Bibliography

BELIN, D., VASSALLI, J., COMBÃPINE, C., GODEAU, F., NAGAMINE, Y., REICH, E., KOCHER, H. P., & DUVOISIN, R. M. (1985). Cloning, nucleotide sequencing and expression of cDNAs encoding mouse urokinaseâtype plasminogen activator. European Journal of Biochemistry, 148(2), 225â232. Portico.

Authors 8

Dominique BELIN (first)
Jean‐Dominique VASSALLI (additional)
Chantal COMBÉPINE (additional)
François GODEAU (additional)
Yoshikuni NAGAMINE (additional)
Edward REICH (additional)
Hans P. KOCHER (additional)
Robert M. DUVOISIN (additional)

References 44 Referenced 128

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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 6:48 p.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 4:47 p.m.)
Indexed	11 months, 2 weeks ago (Sept. 13, 2024, 1:45 p.m.)
Issued	40 years, 5 months ago (April 1, 1985)
Published	40 years, 5 months ago (April 1, 1985)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	40 years, 5 months ago (April 1, 1985)

Funders 0

None

BibTeX

@article{BELIN_1985, title={Cloning, nucleotide sequencing and expression of cDNAs encoding mouse urokinase‐type plasminogen activator}, volume={148}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1985.tb08829.x}, DOI={10.1111/j.1432-1033.1985.tb08829.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={BELIN, Dominique and VASSALLI, Jean‐Dominique and COMBÉPINE, Chantal and GODEAU, François and NAGAMINE, Yoshikuni and REICH, Edward and KOCHER, Hans P. and DUVOISIN, Robert M.}, year={1985}, month=apr, pages={225–232} }

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