DOI: 10.1111/j.1432-1033.1985.tb08714.x. ADP‐ribosylation of phosphorylase kinase and block of phosphate incorporation into the enzyme

ADP‐ribosylation of phosphorylase kinase and block of phosphate incorporation into the enzyme

10.1111/j.1432-1033.1985.tb08714.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Phosphorylase kinase purified from rabbit skeletal muscle was ADP‐ribosylated by hen liver nuclear ADP‐ribosyltransferase. This modification, as was seen in cAMP‐dependent phosphorylation, was observed only in α and β subunits of the phosphorylase kinase and the latter was more rapidly modified. Analysis of the ADP‐ribosylated amino acid residue sequenced in α and β subunits showed that both subunits were modified at the area of the arginine residue. The Km for NAD was 0.10 mM and the pH optimum was 9.0. When the ADP‐ribosylated phosphorylase kinase was phosphorylated by cAMP‐dependent protein kinase, a reduction in phosphate incorporation occurred with increase in the ADP‐ribosylation. ADP‐ribosylation also suppressed autophosphorylation, to a lesser degree than observed with cAMP‐dependent phosphorylation. The ADP‐ribosylation‐dependent reduction of phosphorylation resulted in a suppression of the phosphorylation‐dependent activation of the phosphorylase kinase. These results together with findings of ADP‐ribosyltransferase activity in the rabbit skeletal muscle [Soman, G. et al. (1984) Biochem. Biophys. Res. Commun. 120, 973–980] suggest that ADP‐ribosylation participates in the regulation of the phosphorylase kinase activity through changes in the rate of phosphorylation.

Bibliography

TSUCHIYA, M., TANIGAWA, Y., USHIROYAMA, T., MATSUURA, R., & SHIMOYAMA, M. (1985). ADPâribosylation of phosphorylase kinase and block of phosphate incorporation into the enzyme. European Journal of Biochemistry, 147(1), 33â40. Portico.

Authors 5

Mikako TSUCHIYA (first)
Yoshinori TANIGAWA (additional)
Takahisa USHIROYAMA (additional)
Ryoji MATSUURA (additional)
Makoto SHIMOYAMA (additional)

References 38 Referenced 22

10.1016/S0021-9258(18)93347-8
10.1016/0092-8674(84)90336-2
10.1016/S0021-9258(19)70705-4
10.1016/S0021-9258(17)43510-1
10.1016/0006-291X(83)90442-4
10.1016/0014-5793(83)80970-3
10.1111/j.1432-1033.1979.tb04175.x
10.1016/S0021-9258(19)34204-8
10.1016/S0021-9258(19)77176-2
10.1016/0006-291X(78)91321-9 / Biochem. Biophys. Res. Commun. by Oka J. (1978)
10.1016/0076-6879(74)38046-9
10.1111/j.1432-1033.1973.tb02721.x
10.1016/S0076-6879(62)05243-X
10.1016/S0021-9258(17)34632-X
10.1016/S0021-9258(18)32434-7 / J. Biol. Chem. by Moss J. (1983)
10.1073/pnas.75.8.3621
10.1016/S0021-9258(18)94333-4
10.1016/S0021-9258(18)97126-7
10.1021/bi00850a037
10.1016/S0021-9258(19)52451-6
10.1016/0014-5793(78)80772-8
10.1016/S0021-9258(17)40578-3
10.1016/S0021-9258(17)43521-6
10.1016/S0021-9258(17)30177-1
10.1016/S0021-9258(19)43873-8
{'key': 'e_1_2_3_27_2', 'first-page': '413', 'volume-title': 'Novel ADP‐ribosylations of regulatory enzymes and proteins', 'author': 'Bodley J. W.', 'year': '1980'} / Novel ADP‐ribosylations of regulatory enzymes and proteins by Bodley J. W. (1980)
10.1016/S0021-9258(18)69685-1
10.1016/S0021-9258(17)32874-0
10.1016/S0021-9258(17)33233-7
10.1016/S0021-9258(17)44586-8 / J. Biol. Chem. by King M. M. (1983)
10.1016/S0021-9258(20)82147-4
10.1016/S0006-291X(84)80202-8
10.1016/S0021-9258(17)40137-2
10.1042/bj1620411
10.1016/S0021-9258(17)42962-0 / J. Biol. Chem. by Moss J. (1984)
10.1073/pnas.75.7.3050
10.1016/S0021-9258(17)43413-2
10.1073/pnas.61.4.1428

Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 6:41 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 6:20 p.m.)
Indexed	1 day, 4 hours ago (Sept. 4, 2025, 10:28 a.m.)
Issued	40 years, 7 months ago (Feb. 1, 1985)
Published	40 years, 7 months ago (Feb. 1, 1985)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	40 years, 7 months ago (Feb. 1, 1985)

Funders 0

None

BibTeX

@article{TSUCHIYA_1985, title={ADP‐ribosylation of phosphorylase kinase and block of phosphate incorporation into the enzyme}, volume={147}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1985.tb08714.x}, DOI={10.1111/j.1432-1033.1985.tb08714.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={TSUCHIYA, Mikako and TANIGAWA, Yoshinori and USHIROYAMA, Takahisa and MATSUURA, Ryoji and SHIMOYAMA, Makoto}, year={1985}, month=feb, pages={33–40} }

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