DOI: 10.1111/j.1432-1033.1984.tb08474.x. Preparation of red‐cell‐membrane cytoskeletal constituents and characterisation of protein 4.1

Preparation of red‐cell‐membrane cytoskeletal constituents and characterisation of protein 4.1

10.1111/j.1432-1033.1984.tb08474.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

A new and rapid method is described for the preparation of protein 4.1, the protein which modulates the interaction between spectrin and actin in the membrane cytoskeleton of the red cell. The method is based on the dissociation of purified membrane cytoskeletons in concentrated Tris at neutral pH, followed by gel filtration in the same medium. This procedure also yields spectrin and actin, as well as the fourth cytoskeletal constituent, protein 4.9, in relatively pure form, and ankyrin. Protein 4.1 is monomeric under our conditions of solvent and protein concentration, with a relative molecular mass, as determined from sedimentation equilibrium, of about 78000; its sedimentation coefficient and Stokes' radius are those of a globular, though somewhat asymmetric or flexible molecule. It forms a strong complex with F‐actin and spectrin. Protein 4.9 is also recovered in active form, and will bind strongly to F‐actin.

Bibliography

OHANIAN, V., & GRATZER, W. (1984). Preparation of redâcellâmembrane cytoskeletal constituents and characterisation of protein 4.1. European Journal of Biochemistry, 144(2), 375â379. Portico.

Authors 2

Vasken OHANIAN (first)
Walter GRATZER (additional)

References 39 Referenced 40

10.1021/bi00789a030
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{'key': 'e_1_2_2_20_2', 'first-page': '581', 'volume': '79', 'author': 'Portzehl H.', 'year': '1964', 'journal-title': 'Biochim. Biophys. Acta'} / Biochim. Biophys. Acta by Portzehl H. (1964)
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{'key': 'e_1_2_2_40_2', 'first-page': '279a', 'volume': '97', 'author': 'Siegel D. L.', 'year': '1983', 'journal-title': 'J. Cell. Biol.'} / J. Cell. Biol. by Siegel D. L. (1983)

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 6:30 p.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 8:12 p.m.)
Indexed	4 months, 1 week ago (April 22, 2025, 4:28 a.m.)
Issued	40 years, 11 months ago (Oct. 1, 1984)
Published	40 years, 11 months ago (Oct. 1, 1984)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	40 years, 11 months ago (Oct. 1, 1984)

Funders 0

None

BibTeX

@article{OHANIAN_1984, title={Preparation of red‐cell‐membrane cytoskeletal constituents and characterisation of protein 4.1}, volume={144}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1984.tb08474.x}, DOI={10.1111/j.1432-1033.1984.tb08474.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={OHANIAN, Vasken and GRATZER, Walter}, year={1984}, month=oct, pages={375–379} }

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