DOI: 10.1111/j.1432-1033.1984.tb07945.x. Synthesis of pigment‐binding protein in toluene‐treated <i>Rhodopseudomonas capsulata</i> and in cell‐free systems

Synthesis of pigment‐binding protein in toluene‐treated Rhodopseudomonas capsulata and in cell‐free systems

10.1111/j.1432-1033.1984.tb07945.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Pigment‐binding protein of the facultatively phototrophic bacterium Rhodospeudomonas capsulata could be selectively synthesized in toluene‐treated cells as well as in homologous and heterologous cell‐free translation systems by isolated polysomes. It is shown that the pigment‐binding polypeptides of the light‐harvesting complexes are encoded by messenger RNA of extreme longevity. The dependence of their synthesis on the concomitant synthesis of tetrapyrroles was demonstrated in the toluene‐treated cells.The large Mr‐28000 polypeptide of the reaction center and the Mr‐10000 pigment‐binding polypeptide of the light‐harvesting complex II were found to be synthesized by free (water‐soluble) polysomes without a cleavable ‘leader’ or ‘signal’ peptide [reviewed by W. Wickner (1979) Annu. Rev. Biochem. 48, 23–45]. The Mr‐10000 polypeptide, as synthesized in vitro, was studied in more detail. Unlike the membrane‐assembled polypeptide in vivo it was insoluble in an organic solvent mixture (chloroform/methanol 1:1, v/v). After detergent denaturation in the presence of membrane isolated from the organism it became organic‐solvent‐soluble. Obviously the polypeptide could be induced to assume alternative conformations in which its apolar residues were either exposed to the solvent or buried within. These findings, in agreement with Wickner's hypothesis, indicate that the Mr‐10000 polypeptide may enter the lipid bilayer by a ‘membrane‐triggered’ conformational change.

Bibliography

DIERSTEIN, R. (1984). Synthesis of pigmentâbinding protein in tolueneâtreated Rhodopseudomonas capsulata and in cellâfree systems. European Journal of Biochemistry, 138(3), 509â518. Portico.

Authors 1

Roland DIERSTEIN (first)

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Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 5:59 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 3:36 a.m.)
Indexed	1 year, 7 months ago (Jan. 25, 2024, 10:47 a.m.)
Issued	41 years, 7 months ago (Feb. 1, 1984)
Published	41 years, 7 months ago (Feb. 1, 1984)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	41 years, 7 months ago (Feb. 1, 1984)

Funders 0

None

BibTeX

@article{DIERSTEIN_1984, title={Synthesis of pigment‐binding protein in toluene‐treated Rhodopseudomonas capsulata and in cell‐free systems}, volume={138}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1984.tb07945.x}, DOI={10.1111/j.1432-1033.1984.tb07945.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={DIERSTEIN, Roland}, year={1984}, month=feb, pages={509–518} }

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