DOI: 10.1111/j.1432-1033.1983.tb07727.x. Electron paramagnetic resonance and other properties of hydrogenases isolated from <i>Desulfovibrio vulgaris</i> (strain Hildenborough) and <i>Megasphaera elsdenii</i>

Electron paramagnetic resonance and other properties of hydrogenases isolated from Desulfovibrio vulgaris (strain Hildenborough) and Megasphaera elsdenii

10.1111/j.1432-1033.1983.tb07727.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The hydrogenases of Desulfovibrio vulgaris and Megasphaera elsdenii are compared with respect to some of their physical properties. In addition to Fe the only metal ions that are present in significant amounts are Ni and Cu. From cluster extrusion experiments it follows that the D. vulgaris enzyme contains three 4 Fe‐4 S clusters, while M. elsdenii hydrogenase only releases part of its Fe‐S clusters. The resting D. vulgaris enzyme shows only a small 3 Fe‐xS type of EPR signal (maximum 5% electron equivalent). This amount can be increased to approximately 25% by treatment with ferricyanide, with a concomitant large decrease in activity. The M. elsdenii enzyme shows in its oxidized state a normal Hipip (high‐potential iron‐sulphur protein) type of EPR spectrum. After a reduction/oxidation cycle the D. vulgaris enzyme also shows a weak Hipip type of EPR spectrum. In the reduced state both enzymes show complex spectra. By integration of those spectra it is shown that 1.5 electron equivalents are present. The complex spectra do not arise from nuclear hyperfine interactions but are partially due to electron spin interactions. It is proposed that the spectrum of reduced D. vulgaris hydrogenase consists of a sum of three different ferredoxin‐like spectra.

Bibliography

GRANDE, H. J., DUNHAM, W. R., AVERILL, B., VAN DIJK, C., & SANDS, R. H. (1983). Electron paramagnetic resonance and other properties of hydrogenases isolated from Desulfovibrio vulgaris (strain Hildenborough) and Megasphaera elsdenii. European Journal of Biochemistry, 136(1), 201â207. Portico.

Authors 5

Hans J. GRANDE (first)
William R. DUNHAM (additional)
Bruce AVERILL (additional)
Cees VAN DIJK (additional)
Richard H. SANDS (additional)

References 42 Referenced 57

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{'key': 'e_1_2_3_5_2', 'first-page': '193', 'volume-title': 'The Biological Chemistry of Iron, Proc. NATO ASI at Edmonton, Alberta, Canada', 'author': 'Grande H. J.', 'year': '1982'} / The Biological Chemistry of Iron, Proc. NATO ASI at Edmonton, Alberta, Canada by Grande H. J. (1982)
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{'key': 'e_1_2_3_8_2', 'first-page': '97', 'volume-title': 'Microbiological Production of and Utilisation of Gases (H2, CH4, CO)', 'author': 'Mortenson L. E.', 'year': '1974'} / Microbiological Production of and Utilisation of Gases (H2, CH4, CO) by Mortenson L. E. (1974)
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{'key': 'e_1_2_3_25_2', 'volume-title': 'Materials, Characterisation of Ion Beams', 'author': 'Falkmann S.', 'year': '1977'} / Materials, Characterisation of Ion Beams by Falkmann S. (1977)
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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 5:46 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 4:42 p.m.)
Indexed	1 year, 2 months ago (June 26, 2024, 8:54 p.m.)
Issued	41 years, 10 months ago (Oct. 1, 1983)
Published	41 years, 10 months ago (Oct. 1, 1983)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	41 years, 10 months ago (Oct. 1, 1983)

Funders 0

None

BibTeX

@article{GRANDE_1983, title={Electron paramagnetic resonance and other properties of hydrogenases isolated from Desulfovibrio vulgaris (strain Hildenborough) and Megasphaera elsdenii}, volume={136}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1983.tb07727.x}, DOI={10.1111/j.1432-1033.1983.tb07727.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={GRANDE, Hans J. and DUNHAM, William R. and AVERILL, Bruce and VAN DIJK, Cees and SANDS, Richard H.}, year={1983}, month=oct, pages={201–207} }

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