DOI: 10.1111/j.1432-1033.1983.tb07697.x. Antigenic determinants of human placental and testicular placental‐like alkaline phosphatases as mapped by monoclonal antibodies

Antigenic determinants of human placental and testicular placental‐like alkaline phosphatases as mapped by monoclonal antibodies

10.1111/j.1432-1033.1983.tb07697.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Placental alkaline phosphatase (PLAP) in humans shows a high degree of genetic polymorphism as disclosed by electrophoretic analysis. Human testes contain trace amounts of a PLAP‐like enzyme, that although immunologically cross‐reactive with PLAP, shows unique catalytic properties.As an alternative approach to study enzyme polymorphism we have developed monoclonal antibodies to purified allelic variants of PLAP. Five different monoclonal antibodies are described in this report. The antibodies react with different epitopes on the PLAP molecule. Both conformational dependent and independent determinants are detected. Two epitopes are modified when comparing the S and F allelic variants of PLAP. One epitope is common to PLAP and the intestinal isoenzyme of alkaline phosphatase. The five epitopes appear to be mapped on two rather distant antigenic domains. Combinations of any two antibodies binding to different domains give immunoprecipitates with PLAP on Ouchterlony tests and give good response in sandwich enzyme‐linked immunosorbent assays.A study of PLAP‐like enzyme in 32 individual testis samples indicates differences in four of the epitopes when compared with PLAP. Four types of testicular enzymes can be distinguished based on their reactivities. These results indicate structural differences between the testicular PLAP‐like enzyme and PLAP. These differences are compatible with an underlying genetic mechanism.

Bibliography

MILLÃN, J. L., & STIGBRAND, T. (1983). Antigenic determinants of human placental and testicular placentalâlike alkaline phosphatases as mapped by monoclonal antibodies. European Journal of Biochemistry, 136(1), 1â7. Portico.

Authors 2

José Luis MILLÁN (first)
Torgny STIGBRAND (additional)

References 29 Referenced 63

10.1038/281152a0
10.1042/bj1810067
10.1016/S0021-9258(18)99338-5
10.1016/0009-8981(75)90251-X
{'key': 'e_1_2_3_6_2', 'first-page': '141', 'volume': '41', 'author': 'Fishman W. H.', 'year': '1971', 'journal-title': 'Enzymologia'} / Enzymologia by Fishman W. H. (1971)
10.1111/j.1469-1809.1978.tb00927.x
10.1016/0002-9343(74)90631-7
{'key': 'e_1_2_3_9_2', 'first-page': '93', 'volume': '6', 'author': 'Stigbrand T.', 'year': '1982', 'journal-title': 'Isoenzymes Current Topics in Biological and Medical Research'} / Isoenzymes Current Topics in Biological and Medical Research by Stigbrand T. (1982)
10.1016/0014-5793(81)80943-X
10.1073/pnas.77.5.2857 / Proc. Natl Acad. Sci. USA by Goldstein D. J. (1980)
10.1111/j.1469-1809.1974.tb01837.x
{'key': 'e_1_2_3_13_2', 'first-page': '298', 'volume': '48', 'author': 'Singer R. M.', 'year': '1980', 'journal-title': 'Exp. Cell Biol.'} / Exp. Cell Biol. by Singer R. M. (1980)
10.1016/0012-1606(81)90171-8
{'key': 'e_1_2_3_15_2', 'first-page': '1506', 'volume': '40', 'author': 'Chang C. H.', 'year': '1980', 'journal-title': 'Cancer Res.'} / Cancer Res. by Chang C. H. (1980)
10.1007/BF00304541
10.1038/256495a0
10.1007/BF00569701 / Hum. Genet. by Millán J. L. (1982)
{'key': 'e_1_2_3_19_2', 'first-page': '2444', 'volume': '42', 'author': 'Millán J. L.', 'year': '1982', 'journal-title': 'Cancer Res.'} / Cancer Res. by Millán J. L. (1982)
{'key': 'e_1_2_3_20_2', 'first-page': '59', 'volume': '16', 'author': 'Beckman L.', 'year': '1966', 'journal-title': 'Acta Genet. (Basel)'} / Acta Genet. (Basel) by Beckman L. (1966)
10.1007/BF00485341
10.1016/S0021-9258(17)33367-7
10.4049/jimmunol.123.4.1548 / J. Immunol. by Kearny J. F. (1979)
10.1016/0161-5890(80)90150-9
10.1016/0161-5890(78)90070-6
10.1016/S0076-6879(80)70067-8
10.1016/0161-5890(82)90275-9
10.1093/clinchem/27.12.2014 / Clin. Chem. by Millán J. L. (1981)
10.1073/pnas.78.2.1124
10.1111/j.1432-1033.1981.tb05483.x

Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 5:46 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 4:44 p.m.)
Indexed	1 year, 6 months ago (Feb. 26, 2024, 2:57 a.m.)
Issued	41 years, 11 months ago (Oct. 1, 1983)
Published	41 years, 11 months ago (Oct. 1, 1983)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	41 years, 11 months ago (Oct. 1, 1983)

Funders 0

None

BibTeX

@article{MILL_N_1983, title={Antigenic determinants of human placental and testicular placental‐like alkaline phosphatases as mapped by monoclonal antibodies}, volume={136}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1983.tb07697.x}, DOI={10.1111/j.1432-1033.1983.tb07697.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={MILLÁN, José Luis and STIGBRAND, Torgny}, year={1983}, month=oct, pages={1–7} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        2,
        26
      ]
    ],
    "date-time": "2024-02-26T07:57:46Z",
    "timestamp": 1708934266513
  },
  "reference-count": 29,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            3,
            3
          ]
        ],
        "date-time": "2005-03-03T00:00:00Z",
        "timestamp": 1109808000000
      },
      "content-version": "vor",
      "delay-in-days": 7824,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1983,
        10
      ]
    ]
  },
  "abstract": "<jats:p>Placental alkaline phosphatase (PLAP) in humans shows a high degree of genetic polymorphism as disclosed by electrophoretic analysis. Human testes contain trace amounts of a PLAP\u2010like enzyme, that although immunologically cross\u2010reactive with PLAP, shows unique catalytic properties.</jats:p><jats:p>As an alternative approach to study enzyme polymorphism we have developed monoclonal antibodies to purified allelic variants of PLAP. Five different monoclonal antibodies are described in this report. The antibodies react with different epitopes on the PLAP molecule. Both conformational dependent and independent determinants are detected. Two epitopes are modified when comparing the S and F allelic variants of PLAP. One epitope is common to PLAP and the intestinal isoenzyme of alkaline phosphatase. The five epitopes appear to be mapped on two rather distant antigenic domains. Combinations of any two antibodies binding to different domains give immunoprecipitates with PLAP on Ouchterlony tests and give good response in sandwich enzyme\u2010linked immunosorbent assays.</jats:p><jats:p>A study of PLAP\u2010like enzyme in 32 individual testis samples indicates differences in four of the epitopes when compared with PLAP. Four types of testicular enzymes can be distinguished based on their reactivities. These results indicate structural differences between the testicular PLAP\u2010like enzyme and PLAP. These differences are compatible with an underlying genetic mechanism.</jats:p>",
  "DOI": "10.1111/j.1432-1033.1983.tb07697.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ],
    "date-time": "2005-03-03T22:46:27Z",
    "timestamp": 1109889987000
  },
  "page": "1-7",
  "source": "Crossref",
  "is-referenced-by-count": 63,
  "title": "Antigenic determinants of human placental and testicular placental\u2010like alkaline phosphatases as mapped by monoclonal antibodies",
  "prefix": "10.1111",
  "volume": "136",
  "author": [
    {
      "given": "Jos\u00e9 Luis",
      "family": "MILL\u00c1N",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Torgny",
      "family": "STIGBRAND",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_3_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/281152a0"
    },
    {
      "key": "e_1_2_3_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj1810067"
    },
    {
      "key": "e_1_2_3_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)99338-5"
    },
    {
      "key": "e_1_2_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0009-8981(75)90251-X"
    },
    {
      "key": "e_1_2_3_6_2",
      "first-page": "141",
      "volume": "41",
      "author": "Fishman W. H.",
      "year": "1971",
      "journal-title": "Enzymologia"
    },
    {
      "key": "e_1_2_3_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1469-1809.1978.tb00927.x"
    },
    {
      "key": "e_1_2_3_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0002-9343(74)90631-7"
    },
    {
      "key": "e_1_2_3_9_2",
      "first-page": "93",
      "volume": "6",
      "author": "Stigbrand T.",
      "year": "1982",
      "journal-title": "Isoenzymes Current Topics in Biological and Medical Research"
    },
    {
      "key": "e_1_2_3_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(81)80943-X"
    },
    {
      "issue": "5",
      "key": "e_1_2_3_11_2",
      "doi-asserted-by": "crossref",
      "first-page": "2857",
      "DOI": "10.1073/pnas.77.5.2857",
      "volume": "77",
      "author": "Goldstein D. J.",
      "year": "1980",
      "journal-title": "Proc. Natl Acad. Sci. USA"
    },
    {
      "key": "e_1_2_3_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1469-1809.1974.tb01837.x"
    },
    {
      "key": "e_1_2_3_13_2",
      "first-page": "298",
      "volume": "48",
      "author": "Singer R. M.",
      "year": "1980",
      "journal-title": "Exp. Cell Biol."
    },
    {
      "key": "e_1_2_3_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0012-1606(81)90171-8"
    },
    {
      "key": "e_1_2_3_15_2",
      "first-page": "1506",
      "volume": "40",
      "author": "Chang C. H.",
      "year": "1980",
      "journal-title": "Cancer Res."
    },
    {
      "key": "e_1_2_3_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00304541"
    },
    {
      "key": "e_1_2_3_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/256495a0"
    },
    {
      "key": "e_1_2_3_18_2",
      "doi-asserted-by": "crossref",
      "first-page": "145",
      "DOI": "10.1007/BF00569701",
      "volume": "60",
      "author": "Mill\u00e1n J. L.",
      "year": "1982",
      "journal-title": "Hum. Genet."
    },
    {
      "key": "e_1_2_3_19_2",
      "first-page": "2444",
      "volume": "42",
      "author": "Mill\u00e1n J. L.",
      "year": "1982",
      "journal-title": "Cancer Res."
    },
    {
      "key": "e_1_2_3_20_2",
      "first-page": "59",
      "volume": "16",
      "author": "Beckman L.",
      "year": "1966",
      "journal-title": "Acta Genet. (Basel)"
    },
    {
      "key": "e_1_2_3_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00485341"
    },
    {
      "key": "e_1_2_3_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(17)33367-7"
    },
    {
      "key": "e_1_2_3_23_2",
      "doi-asserted-by": "crossref",
      "first-page": "1548",
      "DOI": "10.4049/jimmunol.123.4.1548",
      "volume": "123",
      "author": "Kearny J. F.",
      "year": "1979",
      "journal-title": "J. Immunol."
    },
    {
      "key": "e_1_2_3_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0161-5890(80)90150-9"
    },
    {
      "key": "e_1_2_3_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0161-5890(78)90070-6"
    },
    {
      "key": "e_1_2_3_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0076-6879(80)70067-8"
    },
    {
      "key": "e_1_2_3_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0161-5890(82)90275-9"
    },
    {
      "issue": "12",
      "key": "e_1_2_3_28_2",
      "doi-asserted-by": "crossref",
      "first-page": "2014",
      "DOI": "10.1093/clinchem/27.12.2014",
      "volume": "27",
      "author": "Mill\u00e1n J. L.",
      "year": "1981",
      "journal-title": "Clin. Chem."
    },
    {
      "key": "e_1_2_3_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.78.2.1124"
    },
    {
      "key": "e_1_2_3_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1981.tb05483.x"
    }
  ],
  "container-title": "European Journal of Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1983.tb07697.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1983.tb07697.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T21:44:03Z",
    "timestamp": 1700775843000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1983.tb07697.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1983,
        10
      ]
    ]
  },
  "references-count": 29,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1983,
          10
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1432-1033.1983.tb07697.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1983.tb07697.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1983,
        10
      ]
    ]
  }
}