DOI: 10.1111/j.1432-1033.1983.tb07692.x. The effect of quercetin on the phosphorylatio activity of the Rous sarcoma virus transforming gene product <i>in vitro</i> and <i>in vivo</i>

The effect of quercetin on the phosphorylatio activity of the Rous sarcoma virus transforming gene product in vitro and in vivo

10.1111/j.1432-1033.1983.tb07692.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The phosphotransferase activity of the Rous sarcoma virus src gene product, pp60src, was inhibited both in vitro and in vivo by the bioflavonoid quercetin. The Ki for the inhibitory effect was in the range of 6–11μM under conditions in vitro. The inhibitory effect of quercetin was competitive towards the nucleotides ATP and GTP as substrates for pp60src and was non‐competitive towards α‐casein as the protein substrate of this kinase activity. In contrast, studies in vitro of the phosphotransferase activity of the catalytic subunit of the cAMP‐dependent protein kinase showed that this flavonoid did not inhibit the phosphorylation of physiological substrates of this enzyme. In cultured cells the half‐maximal inhibition of tyrosine phosphorylation of pp60src as well as the phosphorylation of the Mr= 34 000 protein, a physiological substrate of pp60src, was in the range 0.06–0.08 mM.

Bibliography

GRAZIANI, Y., ERIKSON, E., & ERIKSON, R. L. (1983). The effect of quercetin on the phosphorylatio activity of the Rous sarcoma virus transforming gene product in vitro and in vivo. European Journal of Biochemistry, 135(3), 583â589. Portico.

Authors 3

Yosef GRAZIANI (first)
Eleanor ERIKSON (additional)
Raymond L. ERIKSON (additional)

References 49 Referenced 138

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Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 10:10 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 6:11 a.m.)
Indexed	11 months, 2 weeks ago (Sept. 10, 2024, 6:31 p.m.)
Issued	41 years, 10 months ago (Oct. 1, 1983)
Published	41 years, 10 months ago (Oct. 1, 1983)
Published Online	20 years, 5 months ago (March 4, 2005)
Published Print	41 years, 10 months ago (Oct. 1, 1983)

Funders 0

None

BibTeX

@article{GRAZIANI_1983, title={The effect of quercetin on the phosphorylatio activity of the Rous sarcoma virus transforming gene product in vitro and in vivo}, volume={135}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1983.tb07692.x}, DOI={10.1111/j.1432-1033.1983.tb07692.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={GRAZIANI, Yosef and ERIKSON, Eleanor and ERIKSON, Raymond L.}, year={1983}, month=oct, pages={583–589} }

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