DOI: 10.1111/j.1432-1033.1983.tb07346.x. Membrane Topology of ATP Synthase from Bovine Heart Mitochondira and <i>Escherichia coli</i>

Membrane Topology of ATP Synthase from Bovine Heart Mitochondira and Escherichia coli

10.1111/j.1432-1033.1983.tb07346.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The polypeptides exposed to lipids in the mebranous F0 sector of the mitochondrial and Escherichia coli ATP synthases were labelled with radioactive photoreactive lipids. Highly resolving gel electrophoretic conditions were used in order to separate all the eighteen components forming the bovine heart mitochondrial enzyme. The hydrophobic labelling was performed on fully active and inhibitor‐sensitive ATP synthases. In the mitochondrial enzyme prepared according to Serrano et al. (1976) [J. Biol. Chem. 251, 2453–2461] seven polypeptides of Mr 30500; 11500; 10500; 10000; 9500; 8500 and 4500 were labelled. The major amount of radioactivity was associated with the 30500‐Mr component, which is thought to be the adenine nucleotide carrier. In the preparation of Galante et al., (1979) which almost completely lacks this component [J. Biol. Chem. 254, 12372–12378] nine polypeptides of Mr 25000; 21000; 11500; 10500; 10000; 9500; 9200; 8500 and 4500 were labelled. In the ATPase synthase from E. coli the major amount of labelling was associated with subunit b and only a minor portion with subunit c.

Bibliography

MONTECUCCO, C., DABBENIâSALA, F., FRIEDL, P., & GALANTE, Y. M. (1983). Membrane Topology of ATP Synthase from Bovine Heart Mitochondira and Escherichia coli. European Journal of Biochemistry, 132(1), 189â194. Portico.

Authors 4

Cesare MONTECUCCO (first)
Federica DABBENI‐SALA (additional)
Peter FRIEDL (additional)
Yves M. GALANTE (additional)

References 42 Referenced 41

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Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 5:28 p.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 5:16 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 7:02 p.m.)
Issued	42 years, 5 months ago (April 1, 1983)
Published	42 years, 5 months ago (April 1, 1983)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	42 years, 5 months ago (April 1, 1983)

Funders 0

None

BibTeX

@article{MONTECUCCO_1983, title={Membrane Topology of ATP Synthase from Bovine Heart Mitochondira and Escherichia coli}, volume={132}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1983.tb07346.x}, DOI={10.1111/j.1432-1033.1983.tb07346.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={MONTECUCCO, Cesare and DABBENI‐SALA, Federica and FRIEDL, Peter and GALANTE, Yves M.}, year={1983}, month=apr, pages={189–194} }

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