DOI: 10.1111/j.1432-1033.1983.tb07322.x. An Electron Microscopic Approach to the Quaternary Structure of Mitochondrial F<sub>1</sub>‐ATPase

An Electron Microscopic Approach to the Quaternary Structure of Mitochondrial F1‐ATPase

10.1111/j.1432-1033.1983.tb07322.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The three‐dimensional structure of F1‐ATPase from beef heart mitochondria was investigated by electron microscopic techniques. The presence of high concentrations of nucleotides is essential for preservation of the quaternary structure. When in vistigated under such coinditions, monodisperse F1‐ATPase could not be distinguished from the membrane‐bound enzyme. At low resolution, the particle shape resembles an oblate ellipsoid of revolution with an axial ratio of about 2:1.From several lines of evidence (including field micrographs at higher magnifications, Markham rotational analysis, and tilting experiments), two conclusions may be drawn concerning the three‐dimenwsional fine structure of F1‐ATPase.1. At the periphery of the molecule, six globular protein masses are orientated in a way similar to the chair conformation of cyclohexane. This array is interpretated to be made up of an alternating sequence of α and β subunits.2. Part of the central space is occupied by a seventh protein mass, protrusions of which are likely to be in contact with some of the outer subunits. A γ subunit is supposed to be constituent part of this central protein mass.As a consequence, this model favours a stoichiometry oif

Bibliography

TIEDGE, H., SCHÃFER, G., & MAYER, F. (1983). An Electron Microscopic Approach to the Quaternary Structure of Mitochondrial F1âATPase. European Journal of Biochemistry, 132(1), 37â45. Portico.

Authors 3

Henri TIEDGE (first)
Günter SCHÄFER (additional)
Frank MAYER (additional)

References 33 Referenced 68

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10.1016/S0021-9258(18)33672-X

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 5:28 p.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 5:15 p.m.)
Indexed	1 year, 8 months ago (Nov. 23, 2023, 7:05 p.m.)
Issued	42 years, 4 months ago (April 1, 1983)
Published	42 years, 4 months ago (April 1, 1983)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	42 years, 4 months ago (April 1, 1983)

Funders 0

None

BibTeX

@article{TIEDGE_1983, title={An Electron Microscopic Approach to the Quaternary Structure of Mitochondrial F1‐ATPase}, volume={132}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1983.tb07322.x}, DOI={10.1111/j.1432-1033.1983.tb07322.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={TIEDGE, Henri and SCHÄFER, Günter and MAYER, Frank}, year={1983}, month=apr, pages={37–45} }

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