DOI: 10.1111/j.1432-1033.1983.tb07143.x. On the Phosphorylation of Yeast RNA Polymerases A and B

On the Phosphorylation of Yeast RNA Polymerases A and B

10.1111/j.1432-1033.1983.tb07143.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

In exponentially growing cells, RNA polymerase B is exclusively form BI enzyme with several phosphorylated subunits: B220, B23 and possibly B44.5. In RNA polymerase A an average of fifteen phosphate groups are distributed on the five phosphorylated subunits: A190 (6), A43 (4), A34.5 (2), A23 (1—2) and A19 (1—2). Phosphorylation of enzyme A by a yeast protein kinase in vitro adds less than 1 mol phosphate/mol enzyme but occurs essentially at the physiological sites, as shown by a comparison of the peptide patterns obtained by limited proteolysis of subunits 32P‐labelled in vivo and in vitro. No evidence was found in favour of a modulation of RNA polymerase activity in vitro or in vivo via phosphorylation.

Bibliography

BRÃNT, B., BUHLER, J., SENTENAC, A., & FROMAGEOT, P. (1983). On the Phosphorylation of Yeast RNA Polymerases A and B. European Journal of Biochemistry, 130(2), 247â251. Portico.

Authors 4

Bernadette BRÉNT (first)
Jean‐Marie BUHLER (additional)
André SENTENAC (additional)
Pierre FROMAGEOT (additional)

References 24 Referenced 36

10.1038/261429a0
10.1016/0014-5793(76)80893-9
10.1016/S0021-9258(17)40474-1 / J. Biol. Chem. by Bell G. I. (1977)
10.1021/bi00567a025
10.1016/S0021-9258(19)69611-0
10.1016/S0021-9258(20)79748-6
10.1016/S0021-9258(17)33176-9 / J. Biol. Chem. by Hirsh J. (1976)
10.1021/bi00654a006
10.1016/S0021-9258(17)39913-1 / J. Biol. Chem. by Kranisas E. G. (1977)
10.1016/S0021-9258(19)68504-2
10.1016/0006-291X(81)91553-9 / Biochem. Biophys. Res. Commun. by Lee S. K. (1981)
10.1111/j.1432-1033.1976.tb10372.x
10.1016/S0021-9258(18)34762-8 / J. Biol. Chem. by Riva M. (1982)
10.1016/S0021-9258(18)43484-9
10.1016/S0021-9258(20)79912-6
10.1016/0003-2697(76)90527-3
10.1038/227680a0
10.1016/S0021-9258(19)67936-6
10.1016/S0091-679X(08)60951-6
10.1016/S0021-9258(18)34968-8
10.1016/0006-291X(76)90244-8 / Biochem. Biophys. Res. Commun. by Valenzuela P. (1976)
10.1016/S0021-9258(19)75212-0
10.1016/S0021-9258(18)43760-X
10.1016/0014-5793(73)80782-3

Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 5:21 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:26 p.m.)
Indexed	1 month, 3 weeks ago (July 11, 2025, 6:52 a.m.)
Issued	42 years, 7 months ago (Feb. 1, 1983)
Published	42 years, 7 months ago (Feb. 1, 1983)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	42 years, 7 months ago (Feb. 1, 1983)

Funders 0

None

BibTeX

@article{BR_NT_1983, title={On the Phosphorylation of Yeast RNA Polymerases A and B}, volume={130}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1983.tb07143.x}, DOI={10.1111/j.1432-1033.1983.tb07143.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={BRÉNT, Bernadette and BUHLER, Jean‐Marie and SENTENAC, André and FROMAGEOT, Pierre}, year={1983}, month=feb, pages={247–251} }

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