DOI: 10.1111/j.1432-1033.1983.tb07141.x. Modified Plasma‐Membrane ATPase in Mutants of <i>Saccharomyces cerevisiae</i>

Modified Plasma‐Membrane ATPase in Mutants of Saccharomyces cerevisiae

10.1111/j.1432-1033.1983.tb07141.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Mutations affecting the plasma membrane ATPase of Saccharomyces cerevisiae were obtained by selecting mutants resistant to Dio‐9.In a plasma‐membrane‐enriched fraction of the mutant MG2130, the ATPase activity was resistant to vandate (50% inhibition by 26 μM in the mutant compared to 1.3 μM in the parental strain).Several catalytic properties of the membrane‐bound ATPase were modified by 60–120% in the mutant which had a higher Km for MgATP and was more heatstable, less sensitive to mercurials, and more stimulated by monovalent cations than the parental type.A single mutation is responsible for the phenotypes of four independent allelic mutants. Resistance to Dio‐9 in vivo and resistance to vanadate in vitro segregated together in three tetrads issued from a cross between the wild type and mutant. The mutation is semi‐dominant as shown by expression of the mutant phenotype in a heterozygous diploid resulting from the cross between the wild type and mutant.It is concluded that the pma locus, affected by these mutations, is the structural gene either for the 100000‐Mr subunit of plasma membrane ATPase or for a protein which tightly controls the conformation of the plasma‐membrane ATPase within the membrane.

Bibliography

ULASZEWSKI, S., GRENSON, M., & GOFFEAU, A. (1983). Modified PlasmaâMembrane ATPase in Mutants of Saccharomyces cerevisiae. European Journal of Biochemistry, 130(2), 235â239. Portico.

Authors 3

Stanislaw ULASZEWSKI (first)
Marcelle GRENSON (additional)
André GOFFEAU (additional)

References 27 Referenced 52

10.1007/BF00408804
10.1016/0304-4173(81)90010-0
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{'key': 'e_1_2_1_17_2', 'first-page': '275', 'volume-title': 'Short Reports of the First European Bioenergetics Conference', 'author': 'Goffeau A.', 'year': '1980'} / Short Reports of the First European Bioenergetics Conference by Goffeau A. (1980)
{'key': 'e_1_2_1_18_2', 'first-page': 'B155', 'volume': '90', 'author': 'Ulaszewski S.', 'year': '1982', 'journal-title': 'Arch. Int. Phys. Biochem.'} / Arch. Int. Phys. Biochem. by Ulaszewski S. (1982)
{'key': 'e_1_2_1_19_2', 'first-page': 'B156', 'volume': '90', 'author': 'Ulaszewski S.', 'year': '1982', 'journal-title': 'Arch. Int. Phys. Biochem.'} / Arch. Int. Phys. Biochem. by Ulaszewski S. (1982)
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10.1016/0022-2836(80)90055-8
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10.1016/0003-9861(78)90153-4

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 5:21 p.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:23 p.m.)
Indexed	1 year, 2 months ago (June 10, 2024, 8:37 a.m.)
Issued	42 years, 7 months ago (Feb. 1, 1983)
Published	42 years, 7 months ago (Feb. 1, 1983)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	42 years, 7 months ago (Feb. 1, 1983)

Funders 0

None

BibTeX

@article{ULASZEWSKI_1983, title={Modified Plasma‐Membrane ATPase in Mutants of Saccharomyces cerevisiae}, volume={130}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1983.tb07141.x}, DOI={10.1111/j.1432-1033.1983.tb07141.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={ULASZEWSKI, Stanislaw and GRENSON, Marcelle and GOFFEAU, André}, year={1983}, month=feb, pages={235–239} }

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