DOI: 10.1111/j.1432-1033.1982.tb07006.x. Increase of Fidelity of Polypeptide Synthesis by Spermidine in Eukaryotic Cell‐Free Systems

Increase of Fidelity of Polypeptide Synthesis by Spermidine in Eukaryotic Cell‐Free Systems

10.1111/j.1432-1033.1982.tb07006.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The mechanism of spermidine‐induced increase of fidelity of polypeptide synthesis in a wheat germ cell‐free system has been studied. It was found that the increase of fidelity in the presence of spermidine occurred mainly at the level of binding of aminoacyl‐tRNA to ribosomes, that reduction of misreading was more marked at the 5′‐base than at the 3′‐base of the codon and that misreading caused by paromomycin and kanamycin C was not significantly decreased by spermidine. It was deduced from these results that spermidine inhibited low‐frequency misreading more strongly than high‐frequency misreading. In addition, spermidine was found to stimulate the rejection of non‐cognate aminoacyl‐tRNA mainly at an initial discrimination step during the binding of aminoacyl‐tRNA to ribosomes, and slightly at a subsequent GTP‐dependent discrimination step, the so‐called proofreading step.In yeast, rabbit reticulocyte, andArtemia salinacell‐free systems, spermidine was found to increase the fidelity of protein synthesis.

Bibliography

IGARASHI, K., HASHIMOTO, S., MIYAKE, A., KASHIWAGI, K., & HIROSE, S. (1982). Increase of Fidelity of Polypeptide Synthesis by Spermidine in Eukaryotic CellâFree Systems. European Journal of Biochemistry, 128(2â3), 597â604. Portico.

Authors 5

Kazuei IGARASHI (first)
Sanae HASHIMOTO (additional)
Atsuko MIYAKE (additional)
Keiko KASHIWAGI (additional)
Seiyu HIROSE (additional)

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Dates

Type	When
Created	15 years, 1 month ago (July 21, 2010, 12:55 p.m.)
Deposited	6 months, 1 week ago (Feb. 23, 2025, 1:01 a.m.)
Indexed	1 day, 8 hours ago (Sept. 4, 2025, 10:05 a.m.)
Issued	42 years, 10 months ago (Nov. 1, 1982)
Published	42 years, 10 months ago (Nov. 1, 1982)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	42 years, 10 months ago (Nov. 1, 1982)

Funders 0

None

BibTeX

@article{IGARASHI_1982, title={Increase of Fidelity of Polypeptide Synthesis by Spermidine in Eukaryotic Cell‐Free Systems}, volume={128}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1982.tb07006.x}, DOI={10.1111/j.1432-1033.1982.tb07006.x}, number={2–3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={IGARASHI, Kazuei and HASHIMOTO, Sanae and MIYAKE, Atsuko and KASHIWAGI, Keiko and HIROSE, Seiyu}, year={1982}, month=nov, pages={597–604} }

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