DOI: 10.1111/j.1432-1033.1982.tb06859.x. Chemical Cross‐Linking of Elongation Factor G to both Subunits of the 70‐S Ribosomes from <i>Escherichia coli</i>

Chemical Cross‐Linking of Elongation Factor G to both Subunits of the 70‐S Ribosomes from Escherichia coli

10.1111/j.1432-1033.1982.tb06859.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Ribosomal proteins situated at or near the binding site of elongation factor G (EF‐G) on the Escherichia coli ribosome have been identified by use of the bifunctional, cleavable cross‐linker, dimethyl‐4,9‐diaza‐5,8‐dioxo‐6,7‐dihydroxy‐dodecanebisimidate. Five different bimolecular EF‐G · ribosomal‐protein complexes were isolated electrophoretically. The ribosomal proteins found in each of these complexes were identified as the 50‐S‐subunit proteins L6, L7/L12 and L14 and the 30‐S‐subunit proteins S12 and S19. In the presence of thiostrepton, which prevents binding of EF‐G to the ribosome, there was a considerable decrease in the yield of each of these cross‐linked complexes. The data suggest that EF‐G is bound close to the ribosomal subunit interface.

Bibliography

SKÃLD, S. (1982). Chemical CrossâLinking of Elongation Factor G to both Subunits of the 70âS Ribosomes from Escherichia coli. European Journal of Biochemistry, 127(2), 225â229. Portico.

Authors 1

Sven‐Erik SKÖLD (first)

References 30 Referenced 34

10.1021/bi00740a026
10.1073/pnas.71.4.1277
10.1016/S0021-9258(17)40890-8 / J. Biol. Chem. by Maasen J. A. (1978)
10.1016/0022-2836(81)90513-1
10.1016/0014-5793(81)80664-3
10.1111/j.1432-1033.1981.tb05235.x
10.1021/bi00657a006
10.1016/S0300-9084(81)80146-0
10.1016/0003-2697(80)90472-8
10.1021/bi00547a030
10.1016/S0021-9258(19)41496-8
10.1016/0076-6879(74)30026-2
10.1016/0014-5793(79)80061-7
10.1021/bi00835a031
10.1038/227680a0
10.1016/0003-2697(79)90641-9
10.1021/bi00800a009
10.1016/0014-5793(76)80915-5
10.1093/nar/6.12.3891
10.1016/0022-2836(81)90481-2
10.1016/S0021-9258(18)91792-8
10.1007/BF00264801
10.1016/0014-5793(74)80005-0
10.1016/0014-5793(74)80872-0
10.1016/S0021-9258(17)33719-5
10.1016/S0021-9258(19)45679-2
{'key': 'e_1_2_2_28_2', 'first-page': '89', 'volume-title': 'Ribosomes', 'author': 'Traut R. R.', 'year': '1980'} / Ribosomes by Traut R. R. (1980)
{'issue': '4', 'key': 'e_1_2_2_29_2', 'first-page': '56', 'volume': '244', 'author': 'Lake J. A.', 'year': '1981', 'journal-title': 'Sci. Am.'} / Sci. Am. by Lake J. A. (1981)
10.1016/S0021-9258(19)44401-3
10.1073/pnas.69.3.686

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 5:15 p.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 3:53 p.m.)
Indexed	1 year, 9 months ago (Nov. 22, 2023, 4:17 p.m.)
Issued	42 years, 10 months ago (Oct. 1, 1982)
Published	42 years, 10 months ago (Oct. 1, 1982)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	42 years, 10 months ago (Oct. 1, 1982)

Funders 0

None

BibTeX

@article{SK_LD_1982, title={Chemical Cross‐Linking of Elongation Factor G to both Subunits of the 70‐S Ribosomes from Escherichia coli}, volume={127}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1982.tb06859.x}, DOI={10.1111/j.1432-1033.1982.tb06859.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={SKÖLD, Sven‐Erik}, year={1982}, month=oct, pages={225–229} }

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