DOI: 10.1111/j.1432-1033.1982.tb06735.x. Biosynthesis of Dog Fibrinogen

10.1111/j.1432-1033.1982.tb06735.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Dogs were injected with 3H‐labeled L‐amino acids and the incorporation of radioactivity into fibrinogen, its component polypeptide chains and various well characterized fibrinogen fragments, was determined in newly secreted plasma fibrinogen and in nascent fibrinogen isolated from the rough endoplasmic reticulum of hepatocytes. At 15‐20 min after the administration of 3H‐labeled l‐amino acids, radioactive fibrinogen was secreted onto the blood and the specific radioactivities of the Aα, Bβ and y chains were approximately the same at all times of secretion up to 2 h. Newly secreted fibrinogen was almost entirely in its phosphorylated form. At 15‐18 min, a time at which maximal incorporation of radioactivity had occurred in proteins, fibrinogen was isolated from a deoxycholate‐soluble fraction of the rough endoplasmic reticulum by affinity chromatography using columns of fibrin monomer as well as those prepared from monospecific antibodies to dog fibrinogen and to the fibrinogen chains. The nascent fibrinogen thus isolated was further characterized by analyses of thrombin‐ released fibrinopeptides A and B and their tryptic peptides. Furthermore, the nascent fibrinogen was cleaved with cyanogen bromide, the NH2‐terminal disulfide knot was prepared and its component chain fragments as well as the tryptic peptides derived from these fragments were isolated. The specific radioactivities of the various fibrinogen fragments were determined and it is concluded that all three fibrinogen chains are synthesized to the same extent and that, within the rough endoplasmic reticulum, nascent fibrinogen has already been fully disulfide‐bonded and exists as a dimer. In contrast to plasma fibrinogen, the Aa chain of nascent fibrinogen within the rough endoplasmic reticulum is not phosphorylated and the Bβ chain is not sulfated.

Bibliography

KUDRYK, B., OKADA, M., REDMAN, C. M., & BLOMBÃCK, B. (1982). Biosynthesis of Dog Fibrinogen. European Journal of Biochemistry, 125(3), 673â682. Portico.

Authors 4

Bohdan KUDRYK (first)
Masahisa OKADA (additional)
Colvin M. REDMAN (additional)
Birger BLOMBÄCK (additional)

References 45 Referenced 40

10.1111/j.1749-6632.1972.tb16323.x
10.1111/j.1432-1033.1977.tb11704.x
10.1021/ja01639a073
10.1038/193883a0
10.1016/0006-291X(64)90268-2
10.1016/S0021-9258(19)45586-5
10.1016/S0021-9258(19)43575-8
10.1111/j.1432-1033.1979.tb13213.x
{'key': 'e_1_2_3_10_2', 'first-page': '1643', 'volume': '358', 'author': 'Henschen A.', 'year': '1977', 'journal-title': "Hoppe-Seyler' Z. Physiol. Chem."} / Hoppe-Seyler' Z. Physiol. Chem. by Henschen A. (1977)
10.1021/bi00568a011
10.1021/bi00568a011
10.1021/bi00591a024
{'key': 'e_1_2_3_14_2', 'first-page': '935', 'volume': '358', 'author': 'Lottspeich F.', 'year': '1977', 'journal-title': "Hoppe-Seyler' Z. Physiol. Chem."} / Hoppe-Seyler' Z. Physiol. Chem. by Lottspeich F. (1977)
{'key': 'e_1_2_3_15_2', 'first-page': '1951', 'volume': '360', 'author': 'Henschen A.', 'year': '1979', 'journal-title': "Hoppe-Seyler' Z. Physiol. Chem."} / Hoppe-Seyler' Z. Physiol. Chem. by Henschen A. (1979)
10.1016/0006-291X(80)90056-X
10.1111/j.1749-6632.1980.tb47253.x
10.1073/pnas.78.1.303
10.1016/S0021-9258(19)68822-8 / J. Biol. Chem. by Crabtree G. R. (1981)
10.1083/jcb.67.3.835
10.1016/S0021-9258(18)50469-5
{'key': 'e_1_2_3_22_2', 'first-page': '430a', 'volume': '83', 'author': 'Kudryk B.', 'year': '1979', 'journal-title': 'J. Cell. Biol.'} / J. Cell. Biol. by Kudryk B. (1979)
{'key': 'e_1_2_3_23_2', 'first-page': '299', 'volume': '12', 'author': 'Blombäck B.', 'year': '1958', 'journal-title': 'Ark. Kemi'} / Ark. Kemi by Blombäck B. (1958)
{'key': 'e_1_2_3_24_2', 'first-page': '415', 'volume': '10', 'author': 'Blombäck B.', 'year': '1956', 'journal-title': 'Ark. Kemi'} / Ark. Kemi by Blombäck B. (1956)
10.1016/0304-4165(66)90437-5
10.1016/S0021-9258(19)41125-3
10.1016/0049-3848(73)90025-X
10.1016/0049-3848(73)90040-6
10.1083/jcb.66.1.42
10.4049/jimmunol.117.5_Part_1.1482
10.1016/B978-0-12-470205-9.50011-3
10.1111/j.1432-1033.1974.tb03599.x
10.1038/218134a0
10.1016/0049-3848(75)90106-1
10.1016/0167-4838(82)90013-9
10.3181/00379727-110-27635
10.1111/j.1423-0410.1963.tb04169.x
10.1016/0006-291X(74)90335-0
10.1016/0006-2944(75)90092-7
10.1016/0006-291X(68)90528-7
10.1016/0006-291X(68)90254-4
10.1126/science.164.3879.584
10.1073/pnas.56.2.608
10.1083/jcb.67.3.852
10.1042/bj1780501
{'key': 'e_1_2_3_46_2', 'first-page': '26', 'volume': '38', 'author': 'Alving B.', 'year': '1977', 'journal-title': 'Proc. VIth Int. Cong Thromb. Haem.'} / Proc. VIth Int. Cong Thromb. Haem. by Alving B. (1977)

Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 5:11 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 11:38 a.m.)
Indexed	11 months, 3 weeks ago (Sept. 11, 2024, 12:35 a.m.)
Issued	43 years, 2 months ago (July 1, 1982)
Published	43 years, 2 months ago (July 1, 1982)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	43 years, 2 months ago (July 1, 1982)

Funders 0

None

BibTeX

@article{KUDRYK_1982, title={Biosynthesis of Dog Fibrinogen: Characterization of Nascent Fibrinogen in the Rough Endoplasmic Reticulum}, volume={125}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1982.tb06735.x}, DOI={10.1111/j.1432-1033.1982.tb06735.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={KUDRYK, Bohdan and OKADA, Masahisa and REDMAN, Colvin M. and BLOMBÄCK, Birger}, year={1982}, month=jul, pages={673–682} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        9,
        11
      ]
    ],
    "date-time": "2024-09-11T04:35:32Z",
    "timestamp": 1726029332314
  },
  "reference-count": 45,
  "publisher": "Wiley",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            3,
            3
          ]
        ],
        "date-time": "2005-03-03T00:00:00Z",
        "timestamp": 1109808000000
      },
      "content-version": "vor",
      "delay-in-days": 8281,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1982,
        7
      ]
    ]
  },
  "abstract": "<jats:p>Dogs were injected with 3H\u2010labeled L\u2010amino acids and the incorporation of radioactivity into fibrinogen, its component polypeptide chains and various well characterized fibrinogen fragments, was determined in newly secreted plasma fibrinogen and in nascent fibrinogen isolated from the rough endoplasmic reticulum of hepatocytes. At 15\u201020 min after the administration of <jats:sup>3</jats:sup>H\u2010labeled <jats:sc>l</jats:sc>\u2010amino acids, radioactive fibrinogen was secreted onto the blood and the specific radioactivities of the A\u03b1, B\u03b2 and y chains were approximately the same at all times of secretion up to 2 h. Newly secreted fibrinogen was almost entirely in its phosphorylated form. At 15\u201018 min, a time at which maximal incorporation of radioactivity had occurred in proteins, fibrinogen was isolated from a deoxycholate\u2010soluble fraction of the rough endoplasmic reticulum by affinity chromatography using columns of fibrin monomer as well as those prepared from monospecific antibodies to dog fibrinogen and to the fibrinogen chains. The nascent fibrinogen thus isolated was further characterized by analyses of thrombin\u2010 released fibrinopeptides A and B and their tryptic peptides. Furthermore, the nascent fibrinogen was cleaved with cyanogen bromide, the NH<jats:sub>2</jats:sub>\u2010terminal disulfide knot was prepared and its component chain fragments as well as the tryptic peptides derived from these fragments were isolated. The specific radioactivities of the various fibrinogen fragments were determined and it is concluded that all three fibrinogen chains are synthesized to the same extent and that, within the rough endoplasmic reticulum, nascent fibrinogen has already been fully disulfide\u2010bonded and exists as a dimer. In contrast to plasma fibrinogen, the Aa chain of nascent fibrinogen within the rough endoplasmic reticulum is not phosphorylated and the B\u03b2 chain is not sulfated.</jats:p>",
  "DOI": "10.1111/j.1432-1033.1982.tb06735.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ],
    "date-time": "2005-03-03T22:11:05Z",
    "timestamp": 1109887865000
  },
  "page": "673-682",
  "source": "Crossref",
  "is-referenced-by-count": 40,
  "title": "Biosynthesis of Dog Fibrinogen",
  "prefix": "10.1111",
  "volume": "125",
  "author": [
    {
      "given": "Bohdan",
      "family": "KUDRYK",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Masahisa",
      "family": "OKADA",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Colvin M.",
      "family": "REDMAN",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Birger",
      "family": "BLOMB\u00c4CK",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_3_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1749-6632.1972.tb16323.x"
    },
    {
      "key": "e_1_2_3_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1977.tb11704.x"
    },
    {
      "key": "e_1_2_3_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ja01639a073"
    },
    {
      "key": "e_1_2_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/193883a0"
    },
    {
      "key": "e_1_2_3_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(64)90268-2"
    },
    {
      "key": "e_1_2_3_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)45586-5"
    },
    {
      "key": "e_1_2_3_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)43575-8"
    },
    {
      "key": "e_1_2_3_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1979.tb13213.x"
    },
    {
      "key": "e_1_2_3_10_2",
      "first-page": "1643",
      "volume": "358",
      "author": "Henschen A.",
      "year": "1977",
      "journal-title": "Hoppe-Seyler' Z. Physiol. Chem."
    },
    {
      "key": "e_1_2_3_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00568a011"
    },
    {
      "key": "e_1_2_3_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00568a011"
    },
    {
      "key": "e_1_2_3_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00591a024"
    },
    {
      "key": "e_1_2_3_14_2",
      "first-page": "935",
      "volume": "358",
      "author": "Lottspeich F.",
      "year": "1977",
      "journal-title": "Hoppe-Seyler' Z. Physiol. Chem."
    },
    {
      "key": "e_1_2_3_15_2",
      "first-page": "1951",
      "volume": "360",
      "author": "Henschen A.",
      "year": "1979",
      "journal-title": "Hoppe-Seyler' Z. Physiol. Chem."
    },
    {
      "key": "e_1_2_3_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(80)90056-X"
    },
    {
      "key": "e_1_2_3_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1749-6632.1980.tb47253.x"
    },
    {
      "key": "e_1_2_3_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.78.1.303"
    },
    {
      "key": "e_1_2_3_19_2",
      "doi-asserted-by": "crossref",
      "first-page": "9718",
      "DOI": "10.1016/S0021-9258(19)68822-8",
      "volume": "256",
      "author": "Crabtree G. R.",
      "year": "1981",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_3_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.67.3.835"
    },
    {
      "key": "e_1_2_3_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)50469-5"
    },
    {
      "key": "e_1_2_3_22_2",
      "first-page": "430a",
      "volume": "83",
      "author": "Kudryk B.",
      "year": "1979",
      "journal-title": "J. Cell. Biol."
    },
    {
      "key": "e_1_2_3_23_2",
      "first-page": "299",
      "volume": "12",
      "author": "Blomb\u00e4ck B.",
      "year": "1958",
      "journal-title": "Ark. Kemi"
    },
    {
      "key": "e_1_2_3_24_2",
      "first-page": "415",
      "volume": "10",
      "author": "Blomb\u00e4ck B.",
      "year": "1956",
      "journal-title": "Ark. Kemi"
    },
    {
      "key": "e_1_2_3_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-4165(66)90437-5"
    },
    {
      "key": "e_1_2_3_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)41125-3"
    },
    {
      "key": "e_1_2_3_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0049-3848(73)90025-X"
    },
    {
      "key": "e_1_2_3_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0049-3848(73)90040-6"
    },
    {
      "key": "e_1_2_3_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.66.1.42"
    },
    {
      "key": "e_1_2_3_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.4049/jimmunol.117.5_Part_1.1482"
    },
    {
      "key": "e_1_2_3_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/B978-0-12-470205-9.50011-3"
    },
    {
      "key": "e_1_2_3_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1974.tb03599.x"
    },
    {
      "key": "e_1_2_3_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/218134a0"
    },
    {
      "key": "e_1_2_3_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0049-3848(75)90106-1"
    },
    {
      "key": "e_1_2_3_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0167-4838(82)90013-9"
    },
    {
      "key": "e_1_2_3_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.3181/00379727-110-27635"
    },
    {
      "key": "e_1_2_3_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1423-0410.1963.tb04169.x"
    },
    {
      "key": "e_1_2_3_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(74)90335-0"
    },
    {
      "key": "e_1_2_3_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-2944(75)90092-7"
    },
    {
      "key": "e_1_2_3_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(68)90528-7"
    },
    {
      "key": "e_1_2_3_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(68)90254-4"
    },
    {
      "key": "e_1_2_3_42_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.164.3879.584"
    },
    {
      "key": "e_1_2_3_43_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.56.2.608"
    },
    {
      "key": "e_1_2_3_44_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.67.3.852"
    },
    {
      "key": "e_1_2_3_45_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj1780501"
    },
    {
      "key": "e_1_2_3_46_2",
      "first-page": "26",
      "volume": "38",
      "author": "Alving B.",
      "year": "1977",
      "journal-title": "Proc. VIth Int. Cong Thromb. Haem."
    }
  ],
  "container-title": "European Journal of Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1982.tb06735.x",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1982.tb06735.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1982.tb06735.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        9,
        16
      ]
    ],
    "date-time": "2023-09-16T15:38:41Z",
    "timestamp": 1694878721000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1982.tb06735.x"
    }
  },
  "subtitle": [
    "Characterization of Nascent Fibrinogen in the Rough Endoplasmic Reticulum"
  ],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1982,
        7
      ]
    ]
  },
  "references-count": 45,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1982,
          7
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1432-1033.1982.tb06735.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1982.tb06735.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1982,
        7
      ]
    ]
  }
}