DOI: 10.1111/j.1432-1033.1982.tb06720.x. ATP‐Independent Proteolysis of Globin Cyanogen Bromide Peptides in Rabbit Reticulocyte Cell‐Free Extracts

ATP‐Independent Proteolysis of Globin Cyanogen Bromide Peptides in Rabbit Reticulocyte Cell‐Free Extracts

10.1111/j.1432-1033.1982.tb06720.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The catabolism of two rabbit globin cyanogen bromide peptides in cell‐free extracts of ATP‐depleted rabbit reticulocytes has been studied. Proteolysis of the peptides (3533 and 5957 molecular weight) proceeded rapidly in the absence of ATP, had a pH optimum of approximately 7.8, and was inhibited by o‐phenanthroline, N‐ethylmaleimide, cystamine, zinc and cobalt ions, and puromycin peptide high‐molecular‐weight aggregates. Proteolysis of puromycin peptides was inhibited by the rabbit globin cyanogen bromide peptides. The ability of cell‐free extracts of degrade the globin cyanogen bromide peptides decreased with the reticulocyte maturity. Blocking the globin cyanogen bromide peptide amino groups by succinic and maleic anhydride treatment decreased susceptibility to degradation. It is suggested that the globin cyanogen bromide peptides might provide model substrates, replacing puromycin peptides, for the investigation of ATP‐independent proteolytic events.

Bibliography

McKAY, M. J., & HIPKISS, A. R. (1982). ATPâIndependent Proteolysis of Globin Cyanogen Bromide Peptides in Rabbit Reticulocyte CellâFree Extracts. European Journal of Biochemistry, 125(3), 567â573. Portico.

Authors 2

Malcolm J. McKAY (first)
Alan R. HIPKISS (additional)

References 29 Referenced 13

10.1073/pnas.74.1.54
10.1042/bst0060623
10.1042/bst0070729
10.1016/0014-5793(80)80602-8
10.1016/0006-291X(78)91249-4
10.1016/S0021-9258(19)41897-8
10.1146/annurev.bi.45.070176.003531
10.1111/j.1432-1033.1974.tb03578.x
10.1111/j.1432-1033.1976.tb11105.x
{'key': 'e_1_2_1_11_2', 'first-page': '227', 'volume': '75', 'author': 'Goldberg A. L.', 'year': '1980', 'journal-title': 'Ciba Found. Symp.'} / Ciba Found. Symp. by Goldberg A. L. (1980)
Daniels R. S. McKay M. J. Worthington V. C.&Hipkiss A. R.(1982)Biochim. Biophys. Acta in the press.
10.1042/bj1880279
10.1016/0076-6879(67)12189-7
10.1016/S0076-6879(67)11029-X
10.1016/S0076-6879(67)11070-7
10.1016/S0076-6879(72)25016-9
10.1021/bi00902a022
10.1016/S0021-9258(18)94333-4
10.1016/0003-2697(71)90436-2
10.1515/bchm2.1971.352.2.885
10.1515/bchm2.1966.347.1.207
10.1016/0014-5793(74)80248-6
10.1016/S0022-2836(65)80177-2
10.1042/bst0090147
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10.1016/B978-0-12-636150-6.50016-7
10.1042/bst0100096
10.1073/pnas.77.4.1783

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 5:11 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 11:38 a.m.)
Indexed	1 year, 11 months ago (Sept. 17, 2023, 1:11 a.m.)
Issued	43 years, 2 months ago (July 1, 1982)
Published	43 years, 2 months ago (July 1, 1982)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	43 years, 2 months ago (July 1, 1982)

Funders 0

None

BibTeX

@article{McKAY_1982, title={ATP‐Independent Proteolysis of Globin Cyanogen Bromide Peptides in Rabbit Reticulocyte Cell‐Free Extracts}, volume={125}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1982.tb06720.x}, DOI={10.1111/j.1432-1033.1982.tb06720.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={McKAY, Malcolm J. and HIPKISS, Alan R.}, year={1982}, month=jul, pages={567–573} }

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