Abstract
The complete amino acid sequence of the structural protein that constitutes the subunit of the CFAI fimbria has been elucidated. The protein was fragmented by cyanogen bromide cleavage, and by enzymatic cleavage with trypsin. Secondary cleavage of the resulting peptides was performed with chymotrypsin, Staphylococcus aureus protease, and thermolysin. Sequential Edman degradation was performed manually. The CFAI protein comprises 147 amino acid residues, with a molecular weight of 15058.
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Dates
Type | When |
---|---|
Created | 15 years, 1 month ago (July 21, 2010, 11:08 a.m.) |
Deposited | 1 year, 9 months ago (Nov. 23, 2023, 1:47 p.m.) |
Indexed | 1 year ago (Aug. 21, 2024, 6:55 p.m.) |
Issued | 43 years, 3 months ago (May 1, 1982) |
Published | 43 years, 3 months ago (May 1, 1982) |
Published Online | 20 years, 5 months ago (March 3, 2005) |
Published Print | 43 years, 3 months ago (May 1, 1982) |
@article{KLEMM_1982, title={Primary Structure of the CFAl Fimbrial Protein from Human Enterotoxigenic Escherichia coli Strains}, volume={124}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1982.tb06597.x}, DOI={10.1111/j.1432-1033.1982.tb06597.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={KLEMM, Per}, year={1982}, month=may, pages={339–348} }