DOI: 10.1111/j.1432-1033.1982.tb06583.x. Dichlorobenzimidazole‐riboside Inhibition of Nuclear RNA Accumulation Initiated with γ‐Thio Analogues of ATP and GTP

Dichlorobenzimidazole‐riboside Inhibition of Nuclear RNA Accumulation Initiated with γ‐Thio Analogues of ATP and GTP

10.1111/j.1432-1033.1982.tb06583.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The γ‐thio analogues of ATP and GTP, ATP[S] and GTP[S], have been used as affinity probes to measure RNA synthesis initiated in vitro in L cell nuclei. 5,6‐Dichlororibofuranosylbenzimidazole was found to inhibit total RNA synthesis in vitro and the amount bound by mercury‐affinity chromatography. Initiation in vitro was also shown by [35S]ATP[S] and [35S]GTP[S] incorporation in RNA molecules with a large size distribution. Although 66% of RNA molecules labeled with [35S]GTP[S] and 74% of those labeled with [35S]ATP[S] were 3–12 S in size, the remainder of the label was recovered in RNA molecules larger than 12 S and a significant portion in RNA larger than 18 S. The specificity of the initiation process seems to be indicated by the finding of molecules of the size of pre‐tRNA on gel electrophoresis which were labeled with [35S]GTP[S] but not [35S]ATP [S] under our experimental conditions. 5,6‐Dichlororibofuranosylbenzimidazole severely inhibited the incorporation of [35S]GTP [S] and [35S]ATP [S] by all size classes, indicating that it can decrease accumulation of RNA initiated in vitro in the L cell nuclear system.

Bibliography

WINICOV, I., & BUTTON, J. D. (1982). Dichlorobenzimidazoleâriboside Inhibition of Nuclear RNA Accumulation Initiated with Î³âThio Analogues of ATP and GTP. European Journal of Biochemistry, 124(2), 239â244. Portico.

Authors 2

Ilga WINICOV (first)
Jane D. BUTTON (additional)

References 18 Referenced 11

10.1073/pnas.72.3.947
10.1126/science.982026
10.1016/0092-8674(76)90092-1
10.1038/272590a0
10.1073/pnas.74.11.5011
10.1021/bi00575a030
10.1016/0092-8674(78)90030-2
10.1016/S0021-9258(19)43919-7 / J. Biol. Chem. by Hipskind R. A. (1980)
10.1016/0005-2787(80)90014-3
10.1021/ja00752a042
10.1021/bi00668a014
10.1021/bi00639a021
10.1093/nar/5.9.3325
10.1021/bi00638a016
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10.1021/bi00553a032
10.1073/pnas.76.11.5750
10.1016/S0021-9258(19)43904-5 / J. Biol. Chem. by Egyhzi E. (1980)

Dates

Type	When
Created	15 years, 1 month ago (July 21, 2010, 9:48 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 1:45 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 7:10 p.m.)
Issued	43 years, 4 months ago (May 1, 1982)
Published	43 years, 4 months ago (May 1, 1982)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	43 years, 4 months ago (May 1, 1982)

Funders 0

None

BibTeX

@article{WINICOV_1982, title={Dichlorobenzimidazole‐riboside Inhibition of Nuclear RNA Accumulation Initiated with γ‐Thio Analogues of ATP and GTP}, volume={124}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1982.tb06583.x}, DOI={10.1111/j.1432-1033.1982.tb06583.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={WINICOV, Ilga and BUTTON, Jane D.}, year={1982}, month=may, pages={239–244} }

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