DOI: 10.1111/j.1432-1033.1982.tb06560.x. Covalent Structure of Mouse Type‐IV Collagen

Covalent Structure of Mouse Type‐IV Collagen

10.1111/j.1432-1033.1982.tb06560.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Fragment P1 (Mr= 55000) which is located in the carboxy‐terminal portion of the triple‐helical segment of the α1(IV) chain was purified from a pepsin digest of a mouse tumor basement membrane. Peptides produced from PI by cleavage with cyanogen bromide and trypsin were purified and characterised with respect to their size, composition and partial amino acid sequence. Fragment patterns and overlapping sequences allowed the ordering of these peptides within the P1 segment. About 70% of the sequence was determined by Edman degradation. Segments of seven or eight amino acid residues, which lacked the triple‐helical sequence Gly‐Xaa‐Yaa, were found at both ends of fragment PI, explaining the susceptibility of native type IV collagen to pepsin. Two further interruptions of the triple helix were indicated by single deletions of Gly or Yaa positions in the triplet structure (Gly‐Xaa‐Yaa)n. The two 3‐hydroxyproline residues were found in position Xaa and are surrounded by homologous sequences.

Bibliography

SCHUPPAN, D., GLANVILLE, R. W., & TIMPL, R. (1982). Covalent Structure of Mouse TypeâIV Collagen. European Journal of Biochemistry, 123(3), 505â512. Portico.

Authors 3

Detlef SCHUPPAN (first)
Robert W. GLANVILLE (additional)
Rupert TIMPL (additional)

References 30 Referenced 50

10.1016/B978-0-12-363706-2.50008-8
10.1111/j.1432-1033.1981.tb05690.x
10.1111/j.1432-1033.1979.tb12961.x
10.1021/bi00592a026
10.1021/bi00581a028
10.1016/S0021-9258(19)83601-3 / Biol. Chem. by Sage H. (1979)
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10.1111/j.1432-1033.1978.tb12139.x
10.1146/annurev.bi.49.070180.004521
{'key': 'e_1_2_1_11_2', 'volume-title': 'Immunochemistry of the Extracellular Matrix', 'author': 'Timpl R.', 'year': '1982'} / Immunochemistry of the Extracellular Matrix by Timpl R. (1982)
10.1016/0014-5793(80)81191-4
10.1515/bchm2.1981.362.2.943
{'key': 'e_1_2_1_14_2', 'volume-title': 'New Treds in Basement Membrane Research', 'author': 'Glanville R. W.', 'year': '1962'} / New Treds in Basement Membrane Research by Glanville R. W. (1962)
10.1084/jem.145.1.204
{'key': 'e_1_2_1_16_2', 'first-page': '130', 'volume': '7', 'author': 'Timpl R.', 'year': '1979', 'journal-title': 'Front. Matrix Biol.'} / Front. Matrix Biol. by Timpl R. (1979)
10.1021/bi00808a009
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10.1016/0006-291X(77)90722-7
10.1111/j.1432-1033.1972.tb02083.x
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10.1073/pnas.78.2.712
10.1016/S0021-9258(19)68858-7 / J. Biol. Chem. by Dickson L. A. (1981)

Dates

Type	When
Created	15 years, 1 month ago (July 21, 2010, 10:31 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:32 p.m.)
Indexed	1 month, 1 week ago (July 16, 2025, 8:35 a.m.)
Issued	43 years, 4 months ago (April 1, 1982)
Published	43 years, 4 months ago (April 1, 1982)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	43 years, 4 months ago (April 1, 1982)

Funders 0

None

BibTeX

@article{SCHUPPAN_1982, title={Covalent Structure of Mouse Type‐IV Collagen: Isolation, Order and Partial Amino‐Acid Sequence of Cyanogen‐Bromide and Tryptic Peptides of Pepsin Fragment P1 from the αl(IV) Chain}, volume={123}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1982.tb06560.x}, DOI={10.1111/j.1432-1033.1982.tb06560.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={SCHUPPAN, Detlef and GLANVILLE, Robert W. and TIMPL, Rupert}, year={1982}, month=apr, pages={505–512} }

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