DOI: 10.1111/j.1432-1033.1981.tb06409.x. Sub‐etioplast Localization of the Enzyme NADPH: Protochlorophyllide Oxidoreductase

Sub‐etioplast Localization of the Enzyme NADPH: Protochlorophyllide Oxidoreductase

10.1111/j.1432-1033.1981.tb06409.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The membranes from oat etioplasts have been separated by sucrose density gradient centrifugation into a heavy fraction of density 1.20 mg/ml (prolamellar body fraction) and a light fraction of density 1.12 mg/ml (prothylakoid fraction). The light fraction was shown to be enriched in protein, protochlorophyllide and the chloroplast enzyme CF1‐ATPase, but to be deficient in saponins. In the electron microscope this fraction appeared as swollen vesicles. In contrast the heavy fraction appeared considerably enriched in crystalline tubular material and was on analysis found to contain most of the etioplasts' saponin but with reduced protein and pigment. In the same experiments the enzyme NADPH:protochlorephyllide oxidoreductase showed the same distribution pattern as the CF1‐ATPase suggesting its location on the prothylakoids.

Bibliography

LÃTZ, C., RÃPER, U., BEER, N. S., & GRIFFITHS, T. (1981). Subâetioplast Localization of the Enzyme NADPH: Protochlorophyllide Oxidoreductase. European Journal of Biochemistry, 118(2), 347â353. Portico.

Authors 4

Cornelius LÜTZ (first)
Ursula RÖPER (additional)
Neil S. BEER (additional)
Trevor GRIFFITHS (additional)

References 30 Referenced 41

10.1086/407217 / Q. Rev. Biol. by Rozinski J. (1972)
10.1146/annurev.bi.40.070171.001113
10.1242/jcs.9.2.271 / J. Cell. Sci. by Weliburn F. A. M. (1971)
10.1111/j.1469-8137.1974.tb04760.x
10.1242/jcs.15.1.31 / J. Cell. Sci. by Henningsen K. W. (1974)
{'key': 'e_1_2_3_7_2', 'volume-title': 'Ultrastructure and the Biology of Plant Cells.', 'author': 'Gunning B. E. S.', 'year': '1975'} / Ultrastructure and the Biology of Plant Cells. by Gunning B. E. S. (1975)
{'key': 'e_1_2_3_8_2', 'first-page': '481', 'volume-title': 'Chloroplast Development', 'author': 'Lütz C.', 'year': '1978'} / Chloroplast Development by Lütz C. (1978)
Lutz C.(1981)Protoplasma in the press.
Lutz C.(1981)Protoplasma in the press.
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10.1016/S0044-328X(79)80056-2 / Z. Pflanzenphysio by Kesselmeier J. (1979)
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{'key': 'e_1_2_3_17_2', 'first-page': '229', 'volume-title': 'Photoreceptors and Plant Development', 'author': 'Lutz C.', 'year': '1980'} / Photoreceptors and Plant Development by Lutz C. (1980)
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Dates

Type	When
Created	15 years, 1 month ago (July 21, 2010, 10:16 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:11 a.m.)
Indexed	2 weeks, 2 days ago (Aug. 6, 2025, 9:54 a.m.)
Issued	44 years ago (Aug. 1, 1981)
Published	44 years ago (Aug. 1, 1981)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	44 years ago (Aug. 1, 1981)

Funders 0

None

BibTeX

@article{L_TZ_1981, title={Sub‐etioplast Localization of the Enzyme NADPH: Protochlorophyllide Oxidoreductase}, volume={118}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1981.tb06409.x}, DOI={10.1111/j.1432-1033.1981.tb06409.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={LÜTZ, Cornelius and RÖPER, Ursula and BEER, Neil S. and GRIFFITHS, Trevor}, year={1981}, month=aug, pages={347–353} }

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